LOCUS       sequence-19448-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Lawson ND, Scheer N, Pham VN, Kim CH, Chitnis AB, Campos-Ortega JA, 
            Weinstein BM
  TITLE     Notch signaling is required for arterial-venous differentiation 
            during embryonic vascular development.
  JOURNAL   Development. 2001 Oct . 128(19):3675-83.
  PUBMED    11585794
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2001 Oct . 128(19):3675-83."
COMMENT     Sequence Label: 39 p ephrin B2
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(651..669)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(652..669)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(687..703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(687..703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(700..722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    711..727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(735..741,742..759,760..765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    780..801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tccggccgcc atggcggccg cgggaattcg attcatctcc cttatcttcc ccatgaggag
       61 atgcatctgg cttaagtact tcattcgatt tgctgtcctt gccccctaag tcagggtgtt
      121 tgggagggta actggtaggg tagtctttgg gggaaatggg atcagagggg ttttgtccaa
      181 ccttcatgat gattttcatc gacttggttt tacacacccc tccttcctga ttatccagcc
      241 cttccattgt tccattagat gtagagataa tgtagtagtc tttccctctg aagaactcca
      301 ggccccaaag gtttgggctg aactcctgga atttgagggt gaatttgacg tcctggtctg
      361 gcttgacgca gttgagtaga ggcgtgtctg cttttgtgac ctgacaggac tttagctgtt
      421 ccagaggaac catatagagt ttataatact ccactccttc cattgaacca ccttccacac
      481 gtgggcaaac tatgtccatc ttatccccaa tctgcggata cagcaccaga ccctgtcctg
      541 gcacaaactt ggtgttcgtg gtgttccagt aatcactagt gaattcgcgg ccgcctgcag
      601 gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
      661 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      901 gggcgctctt c
//
