LOCUS       sequence-194483-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes dCas9(C) (without VP64) fused to VEGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..805
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3568..3588
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3589..3609
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3628..3654
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3682..4662,4663..4665,4666..6180)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             6184..6204
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6250..6267)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6256..6463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6472..6612
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6472..6601
                     /label=AAV2 ITR
     rep_origin      6687..7142
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6774..6793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6984..7005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7159..7178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7278..7300
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7338..7356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7424..7528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7529..7597,7598..8389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7747..7766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8560..9148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9049..9068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg
      601 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      661 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agctgagcaa
      721 gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt acctggagca
      781 cctgcctgaa atcacttttt ttcaggttgg accggtgcca ccatggtcag ctactgggac
      841 accggggtcc tgctgtgcgc gctgctcagc tgtctgcttc tcacaggatc tagttcaggt
      901 tcaaaattaa aagatcctga actgagttta aaaggcaccc agcacatcat gcaagcaggc
      961 cagacactgc atctccaatg caggggggaa gcagcccata aatggtcttt gcctgaaatg
     1021 gtgagtaagg aaagcgaaag gctgagcata actaaatctg cctgtggaag aaatggcaaa
     1081 caattctgca gtactttaac cttgaacaca gctcaagcaa accacactgg cttctacagc
     1141 tgcaaatatc tagctgtacc tacttcaaag aagaaggaaa cagaatctgc aatctatata
     1201 tttattagtg atacaggtag acctttcgta gagatgtaca gtgaaatccc cgaaattata
     1261 cacatgactg aaggaaggga gctcgtcatt ccctgccggg ttacgtcacc taacatcact
     1321 gttactttaa aaaagtttcc acttgacact ttgatccctg atggaaaacg cataatctgg
     1381 gacagtagaa agggcttcat catatcaaat gcaacgtaca aagaaatagg gcttctgacc
     1441 tgtgaagcaa cagtcaatgg gcatttgtat aagacaaact atctcacaca tcgacaaacc
     1501 aatacaatca tagatgtcca aataagcaca ccacgcccag tcaaattact tagaggccat
     1561 actcttgttc tcaattgtac tgctaccact cccttgaaca cgagagttca aatgacctgg
     1621 agttaccctg atgaaaaaaa taagagagct tccgtaaggc gacgaattga ccaaagcaat
     1681 tcccatgcca acatattcta cagtgttctt actattgaca aaatgcagaa caaagacaaa
     1741 ggactttata cttgtcgtgt aaggagtgga ccatcattca aatctgttaa cacctcagtg
     1801 catatatatg ataaagcatt catcactgtg aaacatcgaa aacagcaggt gcttgaaacc
     1861 gtagctggca agcggtctta ccggctctct atgaaagtga aggcatttcc ctcgccggaa
     1921 gttgtatggt taaaagatgg gttacctgcg actgagaaat ctgctcgcta tttgactcgt
     1981 ggctactcgt taattatcaa ggacgtaact gaagaggatg cagggaatta tacaatcttg
     2041 ctgagcataa aacagtcaaa tgtgtttaaa aacctcactg ccactctaat tgtcaatgtg
     2101 aaaccccaga tttacgaaaa ggctgtgtca tcgtttccag acccggctct ctacccactg
     2161 ggcagcagac aaatcctgac ttgtaccgca tatggtatcc ctcaacctac aatcaagtgg
     2221 ttctggcacc cctgtaacca taatcattcc gaagcaaggt gtgacttttg ttccaataat
     2281 gaagagtcct ttatcctgga tgctgacagc aacatgggaa acagaattga gagcatcact
     2341 cagcgcatgg caataataga aggaaagaat aagatggcta gcaccttggt tgtggctgac
     2401 tctagaattt ctggaatcta catttgcata gcttccaata aagttgggac tgtgggaaga
     2461 aacataagct tttatatcac agatgtgcca aatgggtttc atgttaactt ggaaaaaatg
     2521 ccgacggaag gagaggacct gaaactgtct tgcacagtta acaagttctt atacagagac
     2581 gttacttgga ttttactgcg gacagttaat aacagaacaa tgcactacag tattagcaag
     2641 caaaaaatgg ccatcactaa ggagcactcc atcactctta atcttaccat catgaatgtt
     2701 tccctgcaag attcaggcac ctatgcctgc agagccagga atgtatacac aggggaagaa
     2761 atcctccaga agaaagaaat tacaatcaga gatcaggaag caccatacct cctgcgaaac
     2821 ctcagtgatc acacagtggc catcagcagt tccaccactt tagactgtca tgctaatggt
     2881 gtccccgagc ctcagatcac ttggtttaaa aacaaccaca aaatacaaca agagcctgga
     2941 attattttag gaccaggaag cagcacgctg tttattgaaa gagtcacaga agaggatgaa
     3001 ggtgtctatc actgcaaagc caccaaccag aagggctctg tggaaagttc agcatacctc
     3061 actgttcaag gaacctcgga caagcctaat ctggagctga tcactctaac atgcacctgt
     3121 gtggctgcga ctctcttctg gctcctatta accctcttta tcggcggtgg ctcgggtggt
     3181 ggctcgaaga gcatgtctag catggtgtca gacactagtt gcacattccc ttcatctgat
     3241 ggcatattct ggaagcattg gattcaaacc aaggatgggc agtgtggcag tccattagta
     3301 tcaactagag atgggttcat tgttggtata cactcagcat cgaatttcac caacacaaac
     3361 aattatttca caagcgtgcc gaaaaacttc atggaattgt tgacaaatca ggaggcgcag
     3421 cagtgggtta gtggttggcg attaaatgct gactcagtat tgtggggggg ccataaagtt
     3481 ttcatggtga aacctgaaga gccttttcag ccagttaagg aagcgactca actcatgaat
     3541 cgtcgtcgcc gtcccggcgg tggctcggaa aacctgtatt ttcaggggcc caagaagaag
     3601 aggaaggtgg ggggaggcag cactagttac ccatacgacg taccagatta cgctggaggt
     3661 tctggatccg gcggtggctc gaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     3721 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     3781 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     3841 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     3901 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     3961 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     4021 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     4081 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     4141 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     4201 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     4261 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     4321 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     4381 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     4441 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     4501 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     4561 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     4621 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca aggcccgggg caagagcgac
     4681 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     4741 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     4801 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     4861 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     4921 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     4981 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     5041 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     5101 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     5161 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     5221 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     5281 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     5341 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     5401 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     5461 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     5521 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     5581 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     5641 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     5701 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
     5761 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     5821 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     5881 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     5941 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     6001 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     6061 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
     6121 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
     6181 agccccaaga agaagagaaa ggtggaggcc agcggaggca gctaagaatt cctagagctc
     6241 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6301 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6361 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6421 gcaaggggga ggattgggaa gagaatagca ggcatgctgg ggagcggccg caggaacccc
     6481 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     6541 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     6601 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6661 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     6721 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     6781 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6841 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6901 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6961 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     7021 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     7081 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     7141 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     7201 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7261 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7321 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7381 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8221 taaatctgga gccggtgagc gtggaagccg cggtatcatt gcagcactgg ggccagatgg
     8281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9181 ggccttttgc tggccttttg ctcacatg
//
