LOCUS       sequence-194484-        9306 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pBDKRB2_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1589..1609
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1610..1630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1649..1675
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1703..2683,2684..2686,2687..4201)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4205..4225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4244..4393
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4421..4474
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4490..4876
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4931..4951
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4967..5509
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             5534..5905
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(5933..5950)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5939..6146
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6155..6295
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6155..6284
                     /label=AAV2 ITR
     rep_origin      6370..6825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6457..6476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6667..6688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6842..6861)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6961..6983
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7021..7039)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7107..7211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7212..7280,7281..8072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7430..7449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8243..8831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8732..8751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8903..9188
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
       61 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
      121 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat
      181 tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcg ccaccatgaa
      241 gacgatcatc gccctgagct acatcttctg cctggtattc gccgactaca aggacgatga
      301 tgacgccagc atcgatatgt tctcaccctg gaagatttct atgttccttt ctgtgcgcga
      361 ggacagcgtg cccacaactg cgagttttag cgccgacatg cttaacgtga ccctgcaggg
      421 ccccactctc aacgggacat ttgctcagtc taaatgtcct caagtggaat ggctcggctg
      481 gttgaacact atccagcccc ctttcctttg ggttcttttt gttctggcca cgctggaaaa
      541 catctttgtt ctctctgtgt tttgcctcca taagagctcc tgtacggtgg ccgaaattta
      601 cctgggtaat ttggccgcag cggatctcat tttggcctgt ggcctgcctt tctgggctat
      661 tactatcagc aataattttg attggttgtt tggcgagacc ttgtgtcgcg tggtgaatgc
      721 aattatcagt atgaatctgt acagctcaat ctgcttcctt atgctcgtca gcattgacag
      781 gtacctcgcc cttgtaaaga ctatgagcat gggccgcatg aggggcgtac gctgggctaa
      841 gctctatagc ctggtgattt ggggttgcac cctcctcctc agttcaccta tgctggtctt
      901 ccgaacgatg aaggagtact ctgacgaggg ccacaatgtt accgcatgcg ttatcagtta
      961 cccatctttg atttgggagg tcttcacaaa catgctgctg aacgtggtgg gttttttgct
     1021 ccccctttct gtgattacct tctgtacaat gcagatcatg caagttttgc gaaacaacga
     1081 aatgcaaaaa tttaaggaga tacagaccga gcgaagggcc acagtgctgg tcttggtcgt
     1141 tttgctgttg ttcattatct gttggctgcc tttccagatc agcactttcc tggacacgtt
     1201 gcacagactc ggaatcctga gtagttgtca ggacgaaagg atcatcgacg ttatcactca
     1261 aatcgcgtcc ttcatggcat actccaatag ctgcttgaac ccattggtgt atgttattgt
     1321 gggaaaacgg tttaggaaga agagctggga agtgtaccaa ggggtgtgcc aaaagggagg
     1381 ctgccgatca gagccaatcc aaatggaaaa tagcatgggt accctgagaa cctccattag
     1441 cgtggagcga cagatccaca agctccagga ctgggctggc tctagacaga tcgataccgg
     1501 tggacgcacc ccacccagcc tgggtcccca agatgagtcc tgcaccaccg ccagctcctc
     1561 cctggccaag gacacttcat cgaccggtga gaacctgtac ttccagggcc ccaagaagaa
     1621 gaggaaggtg gggggaggca gcactagtta cccatacgac gtaccagatt acgctggagg
     1681 ttctggatcc ggcggtggct cgaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     1741 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     1801 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     1861 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     1921 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     1981 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     2041 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     2101 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     2161 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     2221 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     2281 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     2341 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     2401 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     2461 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     2521 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     2581 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     2641 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aaggcccggg gcaagagcga
     2701 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     2761 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     2821 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     2881 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     2941 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     3001 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     3061 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     3121 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     3181 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     3241 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     3301 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     3361 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     3421 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     3481 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     3541 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     3601 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     3661 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     3721 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     3781 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     3841 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     3901 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     3961 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     4021 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     4081 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     4141 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     4201 cagccccaag aagaagagaa aggtggaggc cagcggacgg gctgacgcat tggacgattt
     4261 tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca tgcttggttc
     4321 ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg atgatttcga
     4381 cctggacatg ctgattaacg gtaccgctag cggcagtgga gagggcagag gaagtctgct
     4441 aacatgcggt gacgtcgagg agaatcctgg cccagtgagc aagcttatgg cttcaaactt
     4501 tactcagttc gtgctcgtgg acaatggtgg gacaggggat gtgacagtgg ctccttctaa
     4561 tttcgctaat ggggtggcag agtggatcag ctccaactca cggagccagg cctacaaggt
     4621 gacatgcagc gtcaggcagt ctagtgccca gaagagaaag tataccatca aggtggaggt
     4681 ccccaaagtg gctacccaga cagtgggcgg agtcgaactg cctgtcgccg cttggaggtc
     4741 ctacctgaac atggagctca ctatcccaat tttcgctacc aattctgact gtgaactcat
     4801 cgtgaaggca atgcaggggc tcctcaaaga cggtaatcct atcccttccg ccatcgccgc
     4861 taactcaggt atctacagcg ctggaggagg tggaagcgga ggaggaggaa gcggaggagg
     4921 aggtagcgga cctaagaaaa agaggaaggt ggcggccgct ggatcccctt cagggcagat
     4981 cagcaaccag gccctggctc tggcccctag ctccgctcca gtgctggccc agactatggt
     5041 gccctctagt gctatggtgc ctctggccca gccacctgct ccagcccctg tgctgacccc
     5101 aggaccaccc cagtcactga gcgctccagt gcccaagtct acacaggccg gcgaggggac
     5161 tctgagtgaa gctctgctgc acctgcagtt cgacgctgat gaggacctgg gagctctgct
     5221 ggggaacagc accgatcccg gagtgttcac agatctggcc tccgtggaca actctgagtt
     5281 tcagcagctg ctgaatcagg gcgtgtccat gtctcatagt acagccgaac caatgctgat
     5341 ggagtacccc gaagccatta cccggctggt gaccggcagc cagcggcccc ccgaccccgc
     5401 tccaactccc ctgggaacca gcggcctgcc taatgggctg tccggagatg aagacttctc
     5461 aagcatcgct gatatggact ttagtgccct gctgtcacag atttcctcta gtgggcaggg
     5521 aggaggtgga agcggcttca gcgtggacac cagtgccctg ctggacctgt tcagcccctc
     5581 ggtgaccgtg cccgacatga gcctgcctga ccttgacagc agcctggcca gtatccaaga
     5641 gctcctgtct ccccaggagc cccccaggcc tcccgaggca gagaacagca gcccggattc
     5701 agggaagcag ctggtgcact acacagcgca gccgctgttc ctgctggacc ccggctccgt
     5761 ggacaccggg agcaacgacc tgccggtgct gtttgagctg ggagagggct cctacttctc
     5821 cgaaggggac ggcttcgccg aggaccccac catctccctg ctgacaggct cggagcctcc
     5881 caaagccaag gaccccactg tctcctaaga attcctagag ctcgctgatc agcctcgact
     5941 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6001 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6061 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6121 gaagagaata gcaggcatgc tggggagcgg ccgcaggaac ccctagtgat ggagttggcc
     6181 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     6241 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     6301 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     6361 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6421 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     6481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     6601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6661 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     6721 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     6781 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     6841 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6901 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6961 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7021 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7081 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7201 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7261 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7321 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7381 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7441 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7501 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7561 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7621 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7681 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7741 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7801 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7861 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7921 agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7981 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8041 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8101 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8161 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8221 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8281 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8341 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8401 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8461 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8521 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8581 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8641 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8701 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8761 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8821 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8881 ttgctcacat gctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
     8941 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
     9001 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     9061 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
     9121 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     9181 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     9241 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     9301 gggggg
//
