LOCUS       sequence-194490-        8836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pBDKRB2_TCS(Q'G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-...
FEATURES             Location/Qualifiers
     source          1..8836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1589..1609
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1610..1630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1649..1675
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1703..2683,2684..2686,2687..4201)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4205..4225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4247..4300
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4301..4777
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             5240..5260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5285..5434
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(5468..5485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5474..5681
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5690..5830
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5690..5819
                     /label=AAV2 ITR
     rep_origin      5905..6360
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5992..6011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6202..6223
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6377..6396)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6496..6518
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6556..6574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6642..6746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6747..6815,6816..7607)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6965..6984)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7778..8366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8267..8286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8438..8723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
       61 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
      121 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat
      181 tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcg ccaccatgaa
      241 gacgatcatc gccctgagct acatcttctg cctggtattc gccgactaca aggacgatga
      301 tgacgccagc atcgatatgt tctcaccctg gaagatttct atgttccttt ctgtgcgcga
      361 ggacagcgtg cccacaactg cgagttttag cgccgacatg cttaacgtga ccctgcaggg
      421 ccccactctc aacgggacat ttgctcagtc taaatgtcct caagtggaat ggctcggctg
      481 gttgaacact atccagcccc ctttcctttg ggttcttttt gttctggcca cgctggaaaa
      541 catctttgtt ctctctgtgt tttgcctcca taagagctcc tgtacggtgg ccgaaattta
      601 cctgggtaat ttggccgcag cggatctcat tttggcctgt ggcctgcctt tctgggctat
      661 tactatcagc aataattttg attggttgtt tggcgagacc ttgtgtcgcg tggtgaatgc
      721 aattatcagt atgaatctgt acagctcaat ctgcttcctt atgctcgtca gcattgacag
      781 gtacctcgcc cttgtaaaga ctatgagcat gggccgcatg aggggcgtac gctgggctaa
      841 gctctatagc ctggtgattt ggggttgcac cctcctcctc agttcaccta tgctggtctt
      901 ccgaacgatg aaggagtact ctgacgaggg ccacaatgtt accgcatgcg ttatcagtta
      961 cccatctttg atttgggagg tcttcacaaa catgctgctg aacgtggtgg gttttttgct
     1021 ccccctttct gtgattacct tctgtacaat gcagatcatg caagttttgc gaaacaacga
     1081 aatgcaaaaa tttaaggaga tacagaccga gcgaagggcc acagtgctgg tcttggtcgt
     1141 tttgctgttg ttcattatct gttggctgcc tttccagatc agcactttcc tggacacgtt
     1201 gcacagactc ggaatcctga gtagttgtca ggacgaaagg atcatcgacg ttatcactca
     1261 aatcgcgtcc ttcatggcat actccaatag ctgcttgaac ccattggtgt atgttattgt
     1321 gggaaaacgg tttaggaaga agagctggga agtgtaccaa ggggtgtgcc aaaagggagg
     1381 ctgccgatca gagccaatcc aaatggaaaa tagcatgggt accctgagaa cctccattag
     1441 cgtggagcga cagatccaca agctccagga ctgggctggc tctagacaga tcgataccgg
     1501 tggacgcacc ccacccagcc tgggtcccca agatgagtcc tgcaccaccg ccagctcctc
     1561 cctggccaag gacacttcat cgaccggtga gaacctgtac ttccagggcc ccaagaagaa
     1621 gaggaaggtg gggggaggca gcactagtta cccatacgac gtaccagatt acgctggagg
     1681 ttctggatcc ggcggtggct cgaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     1741 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     1801 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     1861 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     1921 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     1981 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     2041 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     2101 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     2161 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     2221 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     2281 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     2341 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     2401 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     2461 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     2521 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     2581 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     2641 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aaggcccggg gcaagagcga
     2701 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     2761 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     2821 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     2881 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     2941 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     3001 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     3061 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     3121 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     3181 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     3241 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     3301 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     3361 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     3421 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     3481 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     3541 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     3601 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     3661 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     3721 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     3781 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     3841 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     3901 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     3961 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     4021 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     4081 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     4141 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     4201 cagccccaag aagaagagaa aggtggaggc cagcagcggc agtggagagg gcagaggaag
     4261 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca atgatcagtc tgattgcggc
     4321 gttagcggta gattacgtta tcggcatgga aaacgccatg ccgtggaacc tgcctgccga
     4381 tctcgcctgg tttaaacgca acaccttaaa taaacccgtg attatgggcc gccatacctg
     4441 ggaatcaatc ggtcgtccgt tgccaggacg caaaaatatt atcctcagca gtcaaccgag
     4501 tacggacgat cgcgtaacgt gggtgaagtc ggtggatgaa gccatcgcgg cgtgtggtga
     4561 cgtaccagaa atcatggtga ttggcggcgg tcgcgttatt gaacagttct tgccaaaagc
     4621 gcaaaaactg tatctgacgc atatcgacgc agaagtggaa ggcgacaccc atttcccgga
     4681 ttacgagccg gatgactggg aatcggtatt cagcgaattc cacgatgctg atgcgcagaa
     4741 ctctcacagc tattgctttg agattctgga gcggcgaggt ggaggaggtt caggaggtgg
     4801 aggctctgga ggtggaggca gctccaaaac catcgttctt tcggtcggcg aggctactcg
     4861 cactctgact gagatccagt ccaccgcaga ccgtcagatc ttcgaagaga aggtcgggcc
     4921 tctggtgggt cggctgcgcc tcacggcttc gctccgtcaa aacggagcca agaccgcgta
     4981 tcgcgtcaac ctaaaactgg atcaggcgga cgtcgttgat tccggacttc cgaaagtgcg
     5041 ctacactcag gtatggtcgc acgacgtgac aatcgttgcg aatagcaccg aggcctcgcg
     5101 caaatcgttg tacgatttga ccaagtccct cgtcgcgacc tcgcaggtcg aagatcttgt
     5161 cgtcaacctt gtgccgctgg gccgtagcgc tggaggaggt ggaagcggag gaggaggaag
     5221 cggaggagga ggtagcggac ctaagaaaaa gaggaaggtg gcggccgctg gatccggacg
     5281 ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc tcgatgattt
     5341 tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca tgctcggcag
     5401 tgacgccctt gatgatttcg acctggacat gctgattaac taagaattcc tagagctcgc
     5461 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5521 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5581 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5641 aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta
     5701 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     5761 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     5821 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5881 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5941 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     6001 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6061 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6121 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6181 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     6241 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     6301 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     6361 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     6421 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     6481 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6541 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6601 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6661 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6721 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6781 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6841 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6901 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6961 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7021 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7081 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7141 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7201 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7261 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7321 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7381 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7441 aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta
     7501 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7561 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7621 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7681 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7741 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7801 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7861 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7921 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7981 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8041 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8101 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8161 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8221 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8281 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8341 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8401 ccttttgctg gccttttgct cacatgctag aggtacccgt tacataactt acggtaaatg
     8461 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
     8521 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     8581 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     8641 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     8701 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     8761 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     8821 cgatgggggc gggggg
//
