LOCUS       sequence-194491-        9014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pBDKRB2_TCS(Q'G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-...
FEATURES             Location/Qualifiers
     source          1..9014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..804
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             2177..2197
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2198..2218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2237..2263
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2291..3271,3272..3274,3275..4789)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4793..4813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4835..4888
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4889..5365
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             5828..5848
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5873..6022
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(6056..6073)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6062..6269
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6278..6418
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6278..6407
                     /label=AAV2 ITR
     rep_origin      6493..6948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6580..6599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6790..6811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6965..6984)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7084..7106
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7144..7162)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7230..7334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7335..7403,7404..8195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7553..7572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8366..8954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8855..8874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      601 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      661 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
      721 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
      781 ctgcctgaaa tcactttttt tcaggttgga ccggtgcgcc accatgaaga cgatcatcgc
      841 cctgagctac atcttctgcc tggtattcgc cgactacaag gacgatgatg acgccagcat
      901 cgatatgttc tcaccctgga agatttctat gttcctttct gtgcgcgagg acagcgtgcc
      961 cacaactgcg agttttagcg ccgacatgct taacgtgacc ctgcagggcc ccactctcaa
     1021 cgggacattt gctcagtcta aatgtcctca agtggaatgg ctcggctggt tgaacactat
     1081 ccagccccct ttcctttggg ttctttttgt tctggccacg ctggaaaaca tctttgttct
     1141 ctctgtgttt tgcctccata agagctcctg tacggtggcc gaaatttacc tgggtaattt
     1201 ggccgcagcg gatctcattt tggcctgtgg cctgcctttc tgggctatta ctatcagcaa
     1261 taattttgat tggttgtttg gcgagacctt gtgtcgcgtg gtgaatgcaa ttatcagtat
     1321 gaatctgtac agctcaatct gcttccttat gctcgtcagc attgacaggt acctcgccct
     1381 tgtaaagact atgagcatgg gccgcatgag gggcgtacgc tgggctaagc tctatagcct
     1441 ggtgatttgg ggttgcaccc tcctcctcag ttcacctatg ctggtcttcc gaacgatgaa
     1501 ggagtactct gacgagggcc acaatgttac cgcatgcgtt atcagttacc catctttgat
     1561 ttgggaggtc ttcacaaaca tgctgctgaa cgtggtgggt tttttgctcc ccctttctgt
     1621 gattaccttc tgtacaatgc agatcatgca agttttgcga aacaacgaaa tgcaaaaatt
     1681 taaggagata cagaccgagc gaagggccac agtgctggtc ttggtcgttt tgctgttgtt
     1741 cattatctgt tggctgcctt tccagatcag cactttcctg gacacgttgc acagactcgg
     1801 aatcctgagt agttgtcagg acgaaaggat catcgacgtt atcactcaaa tcgcgtcctt
     1861 catggcatac tccaatagct gcttgaaccc attggtgtat gttattgtgg gaaaacggtt
     1921 taggaagaag agctgggaag tgtaccaagg ggtgtgccaa aagggaggct gccgatcaga
     1981 gccaatccaa atggaaaata gcatgggtac cctgagaacc tccattagcg tggagcgaca
     2041 gatccacaag ctccaggact gggctggctc tagacagatc gataccggtg gacgcacccc
     2101 acccagcctg ggtccccaag atgagtcctg caccaccgcc agctcctccc tggccaagga
     2161 cacttcatcg accggtgaga acctgtactt ccagggcccc aagaagaaga ggaaggtggg
     2221 gggaggcagc actagttacc catacgacgt accagattac gctggaggtt ctggatccgg
     2281 cggtggctcg aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     2341 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     2401 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     2461 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     2521 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     2581 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     2641 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     2701 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     2761 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     2821 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     2881 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     2941 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     3001 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     3061 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     3121 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     3181 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     3241 cgacaacaag gtgctgacca gaagcgacaa ggcccggggc aagagcgaca acgtgccctc
     3301 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     3361 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     3421 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     3481 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     3541 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     3601 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     3661 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     3721 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     3781 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     3841 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     3901 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     3961 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     4021 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     4081 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     4141 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     4201 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     4261 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     4321 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     4381 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     4441 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     4501 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     4561 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     4621 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     4681 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     4741 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca gccccaagaa
     4801 gaagagaaag gtggaggcca gcagcggcag tggagagggc agaggaagtc tgctaacatg
     4861 cggtgacgtc gaggagaatc ctggcccaat gatcagtctg attgcggcgt tagcggtaga
     4921 ttacgttatc ggcatggaaa acgccatgcc gtggaacctg cctgccgatc tcgcctggtt
     4981 taaacgcaac accttaaata aacccgtgat tatgggccgc catacctggg aatcaatcgg
     5041 tcgtccgttg ccaggacgca aaaatattat cctcagcagt caaccgagta cggacgatcg
     5101 cgtaacgtgg gtgaagtcgg tggatgaagc catcgcggcg tgtggtgacg taccagaaat
     5161 catggtgatt ggcggcggtc gcgttattga acagttcttg ccaaaagcgc aaaaactgta
     5221 tctgacgcat atcgacgcag aagtggaagg cgacacccat ttcccggatt acgagccgga
     5281 tgactgggaa tcggtattca gcgaattcca cgatgctgat gcgcagaact ctcacagcta
     5341 ttgctttgag attctggagc ggcgaggtgg aggaggttca ggaggtggag gctctggagg
     5401 tggaggcagc tccaaaacca tcgttctttc ggtcggcgag gctactcgca ctctgactga
     5461 gatccagtcc accgcagacc gtcagatctt cgaagagaag gtcgggcctc tggtgggtcg
     5521 gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag accgcgtatc gcgtcaacct
     5581 aaaactggat caggcggacg tcgttgattc cggacttccg aaagtgcgct acactcaggt
     5641 atggtcgcac gacgtgacaa tcgttgcgaa tagcaccgag gcctcgcgca aatcgttgta
     5701 cgatttgacc aagtccctcg tcgcgacctc gcaggtcgaa gatcttgtcg tcaaccttgt
     5761 gccgctgggc cgtagcgctg gaggaggtgg aagcggagga ggaggaagcg gaggaggagg
     5821 tagcggacct aagaaaaaga ggaaggtggc ggccgctgga tccggacggg ctgacgcatt
     5881 ggacgatttt gatctggata tgctgggaag tgacgccctc gatgattttg accttgacat
     5941 gcttggttcg gatgcccttg atgactttga cctcgacatg ctcggcagtg acgcccttga
     6001 tgatttcgac ctggacatgc tgattaacta agaattccta gagctcgctg atcagcctcg
     6061 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6121 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6181 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6241 tgggaagaga atagcaggca tgctggggag cggccgcagg aacccctagt gatggagttg
     6301 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     6361 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     6421 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     6481 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6541 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     6601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     6721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6781 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     6841 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     6901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     6961 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7021 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7081 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7141 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7201 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7321 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7381 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7441 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7501 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7561 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7621 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7681 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7741 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7861 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7921 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7981 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8041 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8161 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8221 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8281 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8941 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9001 cttttgctca catg
//
