LOCUS       sequence-194496-        9190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to CXCR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pCXCR4_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..9190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1472..1492
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1493..1513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1532..1558
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1586..2566,2567..2569,2570..4084)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4088..4108
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4127..4276
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4304..4357
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4373..4759
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4814..4834
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4850..5392
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             5417..5788
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(5816..5833)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5822..6029
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6038..6178
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6038..6167
                     /label=AAV2 ITR
     rep_origin      6253..6708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6340..6359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6550..6571
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6725..6744)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6844..6866
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6904..6922)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6990..7094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7095..7163,7164..7955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7313..7332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8126..8714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8615..8634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8786..9071
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
       61 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
      121 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat
      181 tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcg ccaccatgaa
      241 gacgatcatc gccctgagct acatcttctg cctggtattc gccgactaca aggacgatga
      301 tgacgccagc atcgatatgg aggggatatc aatctacaca tcagataatt acacggaaga
      361 gatgggttcc ggcgattacg actctatgaa agagccgtgt tttagagagg aaaacgcgaa
      421 ctttaacaag atctttctcc ccaccatcta cagcatcatc tttctcacag gcatcgtagg
      481 gaacggcctg gtcatcctgg tgatgggata ccaaaagaag ctgaggtcaa tgaccgacaa
      541 gtataggctc catctgtccg tggccgacct cctgtttgtg attaccctgc ctttttgggc
      601 agttgacgct gtcgctaatt ggtacttcgg caacttcctc tgtaaggcag tgcacgttat
      661 ctacactgtg aatctttata gttccgtctt gatcttggcc tttatcagtc tcgacaggta
      721 tttggcgatt gtgcacgcta ccaactcaca acgacctaga aaactcctgg ctgagaaggt
      781 ggtttacgtt ggtgtgtgga ttccagctct cctgttgaca ataccagact tcattttcgc
      841 taatgtgagc gaggccgatg acagatacat ttgtgaccga ttttacccaa acgatctgtg
      901 ggtagtggta tttcagttcc aacacattat ggtcgggctg atcttgcccg gcattgtcat
      961 actgtcttgc tactgcatca ttatttctaa gctgtcacac tcaaaaggcc accaaaagag
     1021 gaaggctctg aaaacaacgg tgatcctgat actggccttc ttcgcatgtt ggctgcccta
     1081 ctatatcggc atcagcattg actcatttat actcctggaa attatcaagc agggctgcga
     1141 gttcgagaac accgttcata agtggatttc tataaccgag gccctcgcct tctttcactg
     1201 ttgtttgaat ccgattctct acgcgtttct tggcgccaaa tttaaaacaa gcgcccaaca
     1261 tgcactgaca tcagtgtcta gggggagctc tctgaaaatc ctctccaagg gaaaacgagg
     1321 cggacatagc agtgtcagca ctgagtccga atccagctca tttcatagct ctatcgatac
     1381 cggtggacgc accccaccca gcctgggtcc ccaagatgag tcctgcacca ccgccagctc
     1441 ctccctggcc aaggacactt catcgaccgg tgagaacctg tacttccagg gccccaagaa
     1501 gaagaggaag gtggggggag gcagcactag ttacccatac gacgtaccag attacgctgg
     1561 aggttctgga tccggcggtg gctcgaagcc cgccttcctg agcggcgagc agaaaaaggc
     1621 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     1681 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     1741 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     1801 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     1861 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     1921 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     1981 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     2041 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     2101 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     2161 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     2221 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     2281 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     2341 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     2401 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     2461 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
     2521 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaaggccc ggggcaagag
     2581 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
     2641 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
     2701 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     2761 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
     2821 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     2881 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     2941 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     3001 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     3061 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     3121 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
     3181 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
     3241 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     3301 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
     3361 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
     3421 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     3481 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     3541 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     3601 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
     3661 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     3721 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
     3781 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
     3841 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
     3901 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
     3961 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
     4021 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
     4081 cgacagcccc aagaagaaga gaaaggtgga ggccagcgga cgggctgacg cattggacga
     4141 ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg acatgcttgg
     4201 ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc ttgatgattt
     4261 cgacctggac atgctgatta acggtaccgc tagcggcagt ggagagggca gaggaagtct
     4321 gctaacatgc ggtgacgtcg aggagaatcc tggcccagtg agcaagctta tggcttcaaa
     4381 ctttactcag ttcgtgctcg tggacaatgg tgggacaggg gatgtgacag tggctccttc
     4441 taatttcgct aatggggtgg cagagtggat cagctccaac tcacggagcc aggcctacaa
     4501 ggtgacatgc agcgtcaggc agtctagtgc ccagaagaga aagtatacca tcaaggtgga
     4561 ggtccccaaa gtggctaccc agacagtggg cggagtcgaa ctgcctgtcg ccgcttggag
     4621 gtcctacctg aacatggagc tcactatccc aattttcgct accaattctg actgtgaact
     4681 catcgtgaag gcaatgcagg ggctcctcaa agacggtaat cctatccctt ccgccatcgc
     4741 cgctaactca ggtatctaca gcgctggagg aggtggaagc ggaggaggag gaagcggagg
     4801 aggaggtagc ggacctaaga aaaagaggaa ggtggcggcc gctggatccc cttcagggca
     4861 gatcagcaac caggccctgg ctctggcccc tagctccgct ccagtgctgg cccagactat
     4921 ggtgccctct agtgctatgg tgcctctggc ccagccacct gctccagccc ctgtgctgac
     4981 cccaggacca ccccagtcac tgagcgctcc agtgcccaag tctacacagg ccggcgaggg
     5041 gactctgagt gaagctctgc tgcacctgca gttcgacgct gatgaggacc tgggagctct
     5101 gctggggaac agcaccgatc ccggagtgtt cacagatctg gcctccgtgg acaactctga
     5161 gtttcagcag ctgctgaatc agggcgtgtc catgtctcat agtacagccg aaccaatgct
     5221 gatggagtac cccgaagcca ttacccggct ggtgaccggc agccagcggc cccccgaccc
     5281 cgctccaact cccctgggaa ccagcggcct gcctaatggg ctgtccggag atgaagactt
     5341 ctcaagcatc gctgatatgg actttagtgc cctgctgtca cagatttcct ctagtgggca
     5401 gggaggaggt ggaagcggct tcagcgtgga caccagtgcc ctgctggacc tgttcagccc
     5461 ctcggtgacc gtgcccgaca tgagcctgcc tgaccttgac agcagcctgg ccagtatcca
     5521 agagctcctg tctccccagg agccccccag gcctcccgag gcagagaaca gcagcccgga
     5581 ttcagggaag cagctggtgc actacacagc gcagccgctg ttcctgctgg accccggctc
     5641 cgtggacacc gggagcaacg acctgccggt gctgtttgag ctgggagagg gctcctactt
     5701 ctccgaaggg gacggcttcg ccgaggaccc caccatctcc ctgctgacag gctcggagcc
     5761 tcccaaagcc aaggacccca ctgtctccta agaattccta gagctcgctg atcagcctcg
     5821 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5881 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5941 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6001 tgggaagaga atagcaggca tgctggggag cggccgcagg aacccctagt gatggagttg
     6061 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     6121 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     6181 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     6241 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6301 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     6361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     6481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6541 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     6601 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     6661 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     6721 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6781 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6841 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6901 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6961 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7801 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8761 cttttgctca catgctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct
     8821 gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt
     8881 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8941 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg
     9001 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     9061 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     9121 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     9181 gggggggggg
//
