LOCUS       sequence-194497-        9362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to CXCR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pCXCR4_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..9362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..804
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             2060..2080
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2081..2101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2120..2146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2174..3154,3155..3157,3158..4672)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4676..4696
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4715..4864
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4892..4945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4961..5347
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5402..5422
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5438..5980
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             6005..6376
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(6404..6421)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6410..6617
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6626..6766
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6626..6755
                     /label=AAV2 ITR
     rep_origin      6841..7296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6928..6947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7138..7159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7313..7332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7432..7454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7492..7510)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7578..7682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7683..7751,7752..8543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7901..7920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8714..9302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9203..9222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      601 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      661 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
      721 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
      781 ctgcctgaaa tcactttttt tcaggttgga ccggtgcgcc accatgaaga cgatcatcgc
      841 cctgagctac atcttctgcc tggtattcgc cgactacaag gacgatgatg acgccagcat
      901 cgatatggag gggatatcaa tctacacatc agataattac acggaagaga tgggttccgg
      961 cgattacgac tctatgaaag agccgtgttt tagagaggaa aacgcgaact ttaacaagat
     1021 ctttctcccc accatctaca gcatcatctt tctcacaggc atcgtaggga acggcctggt
     1081 catcctggtg atgggatacc aaaagaagct gaggtcaatg accgacaagt ataggctcca
     1141 tctgtccgtg gccgacctcc tgtttgtgat taccctgcct ttttgggcag ttgacgctgt
     1201 cgctaattgg tacttcggca acttcctctg taaggcagtg cacgttatct acactgtgaa
     1261 tctttatagt tccgtcttga tcttggcctt tatcagtctc gacaggtatt tggcgattgt
     1321 gcacgctacc aactcacaac gacctagaaa actcctggct gagaaggtgg tttacgttgg
     1381 tgtgtggatt ccagctctcc tgttgacaat accagacttc attttcgcta atgtgagcga
     1441 ggccgatgac agatacattt gtgaccgatt ttacccaaac gatctgtggg tagtggtatt
     1501 tcagttccaa cacattatgg tcgggctgat cttgcccggc attgtcatac tgtcttgcta
     1561 ctgcatcatt atttctaagc tgtcacactc aaaaggccac caaaagagga aggctctgaa
     1621 aacaacggtg atcctgatac tggccttctt cgcatgttgg ctgccctact atatcggcat
     1681 cagcattgac tcatttatac tcctggaaat tatcaagcag ggctgcgagt tcgagaacac
     1741 cgttcataag tggatttcta taaccgaggc cctcgccttc tttcactgtt gtttgaatcc
     1801 gattctctac gcgtttcttg gcgccaaatt taaaacaagc gcccaacatg cactgacatc
     1861 agtgtctagg gggagctctc tgaaaatcct ctccaaggga aaacgaggcg gacatagcag
     1921 tgtcagcact gagtccgaat ccagctcatt tcatagctct atcgataccg gtggacgcac
     1981 cccacccagc ctgggtcccc aagatgagtc ctgcaccacc gccagctcct ccctggccaa
     2041 ggacacttca tcgaccggtg agaacctgta cttccagggc cccaagaaga agaggaaggt
     2101 ggggggaggc agcactagtt acccatacga cgtaccagat tacgctggag gttctggatc
     2161 cggcggtggc tcgaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     2221 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     2281 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     2341 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     2401 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     2461 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     2521 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     2581 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     2641 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     2701 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     2761 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     2821 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     2881 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     2941 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     3001 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     3061 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     3121 catcgacaac aaggtgctga ccagaagcga caaggcccgg ggcaagagcg acaacgtgcc
     3181 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     3241 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     3301 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     3361 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     3421 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     3481 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     3541 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     3601 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     3661 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     3721 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     3781 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     3841 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     3901 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     3961 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     4021 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     4081 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     4141 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     4201 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     4261 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     4321 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     4381 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     4441 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     4501 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     4561 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     4621 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acagccccaa
     4681 gaagaagaga aaggtggagg ccagcggacg ggctgacgca ttggacgatt ttgatctgga
     4741 tatgctggga agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct
     4801 tgatgacttt gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat
     4861 gctgattaac ggtaccgcta gcggcagtgg agagggcaga ggaagtctgc taacatgcgg
     4921 tgacgtcgag gagaatcctg gcccagtgag caagcttatg gcttcaaact ttactcagtt
     4981 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa
     5041 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag
     5101 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt
     5161 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa
     5221 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc
     5281 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg
     5341 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg
     5401 acctaagaaa aagaggaagg tggcggccgc tggatcccct tcagggcaga tcagcaacca
     5461 ggccctggct ctggccccta gctccgctcc agtgctggcc cagactatgg tgccctctag
     5521 tgctatggtg cctctggccc agccacctgc tccagcccct gtgctgaccc caggaccacc
     5581 ccagtcactg agcgctccag tgcccaagtc tacacaggcc ggcgagggga ctctgagtga
     5641 agctctgctg cacctgcagt tcgacgctga tgaggacctg ggagctctgc tggggaacag
     5701 caccgatccc ggagtgttca cagatctggc ctccgtggac aactctgagt ttcagcagct
     5761 gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa ccaatgctga tggagtaccc
     5821 cgaagccatt acccggctgg tgaccggcag ccagcggccc cccgaccccg ctccaactcc
     5881 cctgggaacc agcggcctgc ctaatgggct gtccggagat gaagacttct caagcatcgc
     5941 tgatatggac tttagtgccc tgctgtcaca gatttcctct agtgggcagg gaggaggtgg
     6001 aagcggcttc agcgtggaca ccagtgccct gctggacctg ttcagcccct cggtgaccgt
     6061 gcccgacatg agcctgcctg accttgacag cagcctggcc agtatccaag agctcctgtc
     6121 tccccaggag ccccccaggc ctcccgaggc agagaacagc agcccggatt cagggaagca
     6181 gctggtgcac tacacagcgc agccgctgtt cctgctggac cccggctccg tggacaccgg
     6241 gagcaacgac ctgccggtgc tgtttgagct gggagagggc tcctacttct ccgaagggga
     6301 cggcttcgcc gaggacccca ccatctccct gctgacaggc tcggagcctc ccaaagccaa
     6361 ggaccccact gtctcctaag aattcctaga gctcgctgat cagcctcgac tgtgccttct
     6421 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6481 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6541 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat
     6601 agcaggcatg ctggggagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     6661 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     6721 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     6781 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     6841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6901 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7021 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     7081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7141 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     7201 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     7261 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     7321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7501 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7561 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggaa
     8401 gccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8521 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9361 tg
//
