LOCUS       sequence-19450-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 221 pCS 3MT DEST
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        61..79
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     61..78
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             129..158
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             168..197
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             207..236
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             246..275
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             285..314
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             348..377
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    405..529
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(554..559,560..577,578..584)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(704..723)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cagggcttag catgctcgtt ngggaccgcc attctgcctg gggacgtcgg agcaagcttg
       61 atttaggtga cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattta
      121 aagctatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca
      181 tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg
      241 aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt
      301 ctgaagagga cttgaatgaa atggagagct tgggcgacct caccatggag caaaagctca
      361 tttctgaaga ggacttgaat ttagagatct cgaattcaag gatcacaagt ttgtacaaaa
      421 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
      481 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
      541 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
      601 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      661 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      721 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      781 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      841 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgat
//
