LOCUS       sequence-194507-       10155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To over-express IGF2BP1 in Adult Erythroblasts using tissue specific
            promoter with IRES for puromycin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   de Vasconcellos JF, Tumburu L, Byrnes C, Lee YT, Xu PC, Li M, Rabel 
            A, Clarke BA, Guydosh NR, Proia RL, Miller JL
  TITLE     IGF2BP1 overexpression causes fetal-like hemoglobin expression 
            patterns in cultured human adult erythroblasts.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):E5664-E5672. doi: 
            10.1073/pnas.1609552114. Epub 2017 Jun 26.
  PUBMED    28652347
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1609552114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-07-11- 11"; volume: "114"; issue: "28"; pages: 
            "E5664-E5672"
COMMENT     Sequence Label: PLVX_SPTA1_IGF2BP1_IRES_PURO
FEATURES             Location/Qualifiers
     source          1..10155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    join(4905..5479,5480..5482,5483..5491)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5072..5089)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5299..5318
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5511..6110
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5511..5530)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6007..6027
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6124..6712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6177..6197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6595..6606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6919..7552
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7680..7696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7680..7696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7693..7715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7704..7720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7728..7734,7735..7752,7753..7758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7773..7794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7911..7928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8082..8670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8162..8181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8841..9632,9633..9701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9464..9483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9702..9806)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9854..9988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9891..9910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9945..9964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatggtacc agactttcaa gaagagaatg taaaggactc
     2221 tcagtttttc tatgtgagat ccaagaacag gggaaaccac aaatgtagaa tcaaaatctt
     2281 ctcttgtaga tcatggaagt tagattattc aagctgcatt tacactggaa tgaagtttgg
     2341 catggatagc cccactggct tgtagaaagg tctaagagca tcccactatt ttctttttgc
     2401 ttgtttgttt catttttgta agttagtcta aaataactat gcctatggaa aaagtctacc
     2461 tccttttgtt ctccattaaa attaatactt acaataggat gatttatacc atatatatca
     2521 gaagtcagcc atattttcaa ctttcttgtg acatcattgg tttgtcaaat aaggtatcct
     2581 aatttttgtt ttacaaaata ttaatgaaca catcatataa ctttttttgt tccttccatt
     2641 taaatgatta agagtacaac taaatcagat ttttaaagac caaattgttg aggttaggaa
     2701 taaaatctta ttttgtgaaa gactctctag aaaaagccag gataattcgt tgacttaaag
     2761 actgtgaaaa atctgcagtt gagttggatg aagttgttga gctgtatttc ctaaaaatat
     2821 atgggttttt tttttctaat ttttcatcca aaaaccttac tttcattttt taagtcttat
     2881 tgagtaactt tgaaagccag agcctcccaa aactgctgag tcacccagta tctgtaaaac
     2941 ttagcagttg cctcagctga gtatgtcttc taaagataat gtcgattgtg tatggctgat
     3001 gggattctag gaccaagcaa gaggtttttt tttttccccc acatacttaa cgtttctata
     3061 tttctatttg aattcgactg gacagttcca tttgaattat ttctctctct ctctctctct
     3121 ctctgacaca ttttatcttg ccaggttcta aacccctcga gtatgaacaa gctttacatc
     3181 ggcaacctca acgagagcgt gacccccgcg gacttggaga aagtgtttgc ggagcacaag
     3241 atctcctaca gcggccagtt cttggtcaaa tccggctacg ccttcgtgga ctgcccggac
     3301 gagcactggg cgatgaaggc catcgaaact ttctccggga aagtagaatt acaaggaaaa
     3361 cgcttagaga ttgaacattc ggtgcccaaa aaacaaagga gccggaaaat tcaaatccga
     3421 aatattccac cccagctccg atgggaagta ctggacagcc tgctggctca gtatggtaca
     3481 gtagagaact gtgagcaagt gaacaccgag agtgagacgg cagtggtgaa tgtcacctat
     3541 tccaaccggg agcagaccag gcaagccatc atgaagctga atggccacca gttggagaac
     3601 catgccctga aggtctccta catccccgat gagcagatag cacagggacc tgagaatggg
     3661 cgccgagggg gctttggctc tcggggtcag ccccgccagg gctcacctgt ggcagcgggg
     3721 gccccagcca agcagcagca agtggacatc ccccttcggc tcctggtgcc cacccagtat
     3781 gtgggtgcca ttattggcaa ggagggggcc accatccgca acatcacaaa acagacccag
     3841 tccaagatag acgtgcatag gaaggagaac gcaggtgcag ctgaaaaagc catcagtgtg
     3901 cactccaccc ctgagggctg ctcctccgct tgtaagatga tcttggagat tatgcataaa
     3961 gaggctaagg acaccaaaac ggctgacgag gttcccctga agatcctggc ccataataac
     4021 tttgtagggc gtctcattgg caaggaagga cggaacctga agaaggtaga gcaagatacc
     4081 gagacaaaaa tcaccatctc ctcgttgcaa gaccttaccc tttacaaccc tgagaggacc
     4141 atcactgtga agggggccat cgagaattgt tgcagggccg agcaggaaat aatgaagaaa
     4201 gttcgggagg cctatgagaa tgatgtggct gccatgagcc tgcagtctca cctgatccct
     4261 ggcctgaacc tggctgctgt aggtcttttc ccagcttcat ccagcgcagt cccgccgcct
     4321 cccagcagcg ttactggggc tgctccctat agctccttta tgcaggctcc cgagcaggag
     4381 atggtgcagg tgtttatccc cgcccaggca gtgggcgcca tcatcggcaa gaaggggcag
     4441 cacatcaaac agctctcccg gtttgccagc gcctccatca agattgcacc acccgaaaca
     4501 cctgactcca aagttcgtat ggttatcatc actggaccgc cagaggccca attcaaggct
     4561 cagggaagaa tctatggcaa actcaaggag gagaacttct ttggtcccaa ggaggaagtg
     4621 aagctggaga cccacatacg tgtgccagca tcagcagctg gccgggtcat tggcaaaggt
     4681 ggaaaaacgg tgaacgagtt gcagaatttg acggcagctg aggtggtagt accaagagac
     4741 cagacccctg atgagaacga ccaggtcatc gtgaaaatca tcggacattt ctatgccagt
     4801 cagatggctc aacggaagat ccgagacatc ctggcccagg ttaagcagca gcatcagaag
     4861 ggacagagta accaggccca ggcacggagg aagtgaggat cccgcccctc tccctccccc
     4921 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     4981 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     5041 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     5101 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     5161 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     5221 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     5281 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     5341 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     5401 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     5461 tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt
     5521 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     5581 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     5641 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     5701 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     5761 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     5821 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     5881 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5941 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     6001 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     6061 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg
     6121 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6181 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6241 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6301 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6361 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6421 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6481 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     6541 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6601 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6661 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     6721 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6781 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6841 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6901 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6961 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     7021 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     7081 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     7141 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     7201 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     7261 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     7321 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     7381 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7441 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7501 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7561 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7621 aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg
     7681 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7741 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7801 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7861 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7921 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7981 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatcgc
     8281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9841 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9961 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    10021 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    10081 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    10141 tacaaactta gtagt
//
