LOCUS       sequence-194535-       13519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GA biosensor expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13519)
  AUTHORS   Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM
  TITLE     In vivo gibberellin gradients visualized in rapidly elongating 
            tissues.
  JOURNAL   Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9.
  PUBMED    28970478
REFERENCE   2  (bases 1 to 13519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2017 Oct 2. doi: 10.1038/s41477-017-0021-9."
COMMENT     Sequence Label: p16 GPS1
FEATURES             Location/Qualifiers
     source          1..13519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(600..1229)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    2540..2564
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    2729..2750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2765..2770,2771..2788,2789..2795)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2803..2819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2808..2830
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2827..2843
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2827..2843
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4079..4103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5397..5421)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5435..5437,5438..6148)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     complement(5477..5498)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5738..5757)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             6149..6178
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      6246..6887
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(6895..6912)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6895..6911)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6904..6926)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(7493..7745)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(7941..8735)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8052..8071)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8662..8681
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8765..9145)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    9517..9541
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10062..10853
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11099..11687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11588..11607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11841..11858
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11873..12013
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11939..11958)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12099..12121
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12357..12551
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(12617..13519,1..171))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
       61 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
      121 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
      181 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
      241 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
      301 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
      361 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
      421 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
      481 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
      541 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
      601 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
      661 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
      721 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
      781 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
      841 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
      901 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
      961 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
     1021 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
     1081 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
     1141 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
     1201 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
     1261 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
     1321 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
     1381 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
     1441 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
     1501 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
     1561 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
     1621 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
     1681 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
     1741 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
     1801 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
     1861 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
     1921 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
     1981 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
     2041 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
     2101 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
     2161 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
     2221 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
     2281 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
     2341 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
     2401 ttggatcaaa gtactttaaa gtactttgat cccgagggga accctgtggt tggcatgcac
     2461 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
     2521 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
     2581 actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcca atacgcaaac
     2641 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     2701 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     2761 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     2821 ttcacacagg aaacagctat gacatgatta cgaattcgag ctcggtacct ggaaccatct
     2881 tttgggttcc tttcttcttc tctttttttg tttctctctt aaaagtcttt tctcttttca
     2941 agtgcttcaa acaaaactaa tttgttataa agggagtttc tctattttat tatatagcaa
     3001 aaaacttcca aaatttctca tttctgtgtt taaccttttc gtacatcagt tttaaagcac
     3061 agagagctca atgttcttcc aatatcgtta ttaataaatt ttgattaaat tcaatcaaat
     3121 cggagttata ttaccacatg aggttaaagg gccatattaa aaagtctgca cttcatatga
     3181 gcaacaaggc ttttatgtct ttatggttga tttgatggcc catatatgat agttcaaagg
     3241 cccatattaa aaaatgccct aacatgattc gattcgtata taaagacgct taatgtgaca
     3301 agcgaacttg ctcattaggg tttctcatct acgacggcgt ggggtacccg gggatcctct
     3361 agagctcatg atgctcgagg tttctaaagg tgaagaattg tttacgggcg tcgtcccgat
     3421 cctcgtggaa ctcgacgggg atgttaacgg gcataagttt tcggtcagcg gggaagggga
     3481 gggggacgcg acgtatggga agctcactct caagctgatc tgtacgacgg ggaaactccc
     3541 ggtcccgtgg ccgacgctgg tcacgacgct gggatacggg ctccaatgct ttgcgaggta
     3601 tccggaccac atgaaacagc atgacttttt caaatcggcg atgccggagg gatacgtgca
     3661 ggaacggacg atctttttca aagacgatgg gaactataag acgcgggcgg aagtcaagtt
     3721 tgaaggggac acgctcgtca accggatcga actcaagggg attgacttca aagaggatgg
     3781 gaacatactc ggccataagc tcgaatacaa ttacaactcg cataacgtat acatcaccgc
     3841 ggataagcaa aagaacggga tcaaagccaa tttcaaaatc cggcataaca tagaggatgg
     3901 gggggtccaa ctggcggatc actatcagca aaacacgccg ataggggatg ggccggtcct
     3961 cctcccggat aaccattacc tctcgtacca aagcgcgctc ttcaaggacc cgaatgagaa
     4021 acgggaccac atggttctcc tggagttcct cacggcggcg ggcataggta ccgatatcac
     4081 aagtttgtac aaaaaagcag gcttcgatga gcttctagct gttcttggtt acaaggttag
     4141 gtcatccgaa atggctgatg ttgctcagaa actcgagcag cttgaagtta tgatgtctaa
     4201 tgttcaagaa gacgatcttt ctcaactcgc tactgagact gttcactata atccggcgga
     4261 gctttacacg tggcttgatt ctatgctcac cgaccttaat cctccgtcgt ctaacgccga
     4321 gtacgatgca agtggcgcgc ctggggccgg ccgaagttct ggaatggctg gaagtgaaga
     4381 agttaatctt attgagagca agacagtggt tcctctcaat acatgggttc taatatccaa
     4441 ctttaagcta gcttacaatc tcctgcgtcg ccctgacgga acttttaacc gtcatctcgc
     4501 agagtttcta gaccggaaag tccctgcaaa tgccaaccct gttaatgggg tcttctcttt
     4561 tgatgtgatc atcgatcgcc aaactaattt gcttagcaga gtttacagac cagctgatgc
     4621 tggcacttca ccaagtatta ctgatctaca gaatcctgtt gatggtgaaa tagtgcctgt
     4681 tattgtcttc tttcatggtg gaagctttgc acattcttct gcaaacagtg ctatttatga
     4741 tactctttgt cgtaggcttg ttggtttgtg tggtgctgtt gttgtctctg tgaattatcg
     4801 tcgtgcacca gagaatcgat acccttgtgc ttatgatgat ggatgggctg ttttgaaatg
     4861 ggtcaactcg agttcttggc ttagaagcaa gaaagactca aaggttcgta ttttcttggc
     4921 gggtgatagc tctgggggta acattgtgca taatgtcgcg gtaagagcgg ttgagtcaag
     4981 gatcgatgtt ttggggaaca ttttgcttaa ccctatgttt ggagggaccg aaagaacgga
     5041 atctgagaaa cgtttggatg ggaagtactt tgttacggtt agagaccgag attggtattg
     5101 gagagcgttt cttcctgagg gtgaagacag agagcatcca gcgtgtagcc cgtttggccc
     5161 gagaagcaag agtctagaag ggttgagttt ccccaagagt cttgtcgttg tagcgggttt
     5221 agatttgatt caagattggc aattgaagta cgcggaaggg ctcaagaaag cgggtcaaga
     5281 ggtgaagctt ctttacttgg agcaagccac tattggcttc tacttattgc ctaacaacaa
     5341 tcacttccat accgttatgg atgagatagc tgcatttgta aacgcagaat gccaacaccc
     5401 agctttcttg tacaaagtgg tgatatcgac tagtgtgagc aagggcgagg agctgttcac
     5461 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5521 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5581 caccggtaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     5641 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5701 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5761 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5821 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
     5881 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     5941 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6001 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgttcaa
     6061 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcctgaccg ccgccgggat
     6121 cactctcggc atggacgagc tgtacaagga acaaaaattg ataagtgagg aagatttata
     6181 agggcccggg acgtccgcgg agatctacgc gtgtcgactc gagatatcca actagttggc
     6241 tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
     6301 ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg ggaaaactgt
     6361 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
     6421 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
     6481 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat
     6541 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
     6601 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca
     6661 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct
     6721 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc
     6781 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt
     6841 ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct tggcactggc
     6901 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6961 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7021 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgccaatcg aggccgacca
     7081 accgcaagcg ttgtcagtgt tgcaaagcgc tctgtgtggg cctactttaa ttgcttccag
     7141 tgttaaattg gcgaaaggca ataatatcgc aaaatattgt gttgtaaaat gtaattatgt
     7201 tttaatttca tggaaatgtt tgagcataat ttttattaat gtactaaatt actgttttgt
     7261 taaatgcaat tttgctttct cgggatttta atatcaaaat ctatttagaa atacacaata
     7321 ttttgttgca ggcttgctgg agaatcgatc tgctatcata aaaattacaa aaaaatttta
     7381 tttgcctcaa ttattttagg attggtatta aggacgctta aattatttgt cgggtcacta
     7441 cgcatcattg tgattgagaa gatcagcgat acgaaatatt cgtagtacta tcgataattt
     7501 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
     7561 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
     7621 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
     7681 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
     7741 ccaaggtttc agggaagctg gaattccggg gtgggcgaag aactccagca tgagatcccc
     7801 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     7861 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     7921 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     7981 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     8041 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     8101 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     8161 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
     8221 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     8281 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     8341 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     8401 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     8461 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     8521 gtggccagcc acgatagccg cgctgcctcg tcctggagtt cattcagggc accggacagg
     8581 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     8641 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     8701 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccggg gaattcgcta
     8761 gagtcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta atgtattggt
     8821 aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa ggcgaaatgg
     8881 cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt ggtacacttt
     8941 tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg gctgcggatc
     9001 aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt gccatgtcct
     9061 acacgccgaa ataaacgacc aaattagtag aaaaataaaa actgactcgg atacttacgt
     9121 cacgtcttgc gcactgattt gaaaaatctc aatataaaca aagacggcca caagaaaaaa
     9181 ccaaaacacc gatattcatt aatcttatct agtttctcaa aaaaattcat atcttccaca
     9241 cgtgaaaatg ccaatttctc agacctacct cggctctgcg aaggcccccg ctggtatcaa
     9301 aagtttttat ttcatccgac atggcgcgac cgacctcaac gagaaggaaa ttgtcgtgaa
     9361 cggtgagaag ctctggggcg tgcaaggttc cggaacgaac atcggtctca atgcaaaagg
     9421 ggaacgccag gctctgttgg cccctcgaaa ttcggcgtta attcagtaca ttaaaaacgt
     9481 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     9541 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     9601 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     9661 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     9721 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     9781 ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     9841 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     9901 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     9961 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
    10021 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
    10081 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
    10141 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
    10201 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
    10261 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
    10321 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
    10381 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
    10441 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
    10501 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
    10561 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
    10621 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
    10681 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
    10741 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
    10801 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
    10861 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
    10921 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
    10981 agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt
    11041 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11221 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    11281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11761 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11821 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
    11881 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    11941 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
    12001 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    12061 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    12121 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
    12181 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
    12241 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
    12301 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
    12361 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
    12421 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
    12481 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
    12541 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
    12601 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
    12661 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
    12721 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
    12781 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
    12841 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
    12901 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
    12961 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
    13021 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
    13081 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
    13141 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
    13201 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
    13261 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
    13321 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
    13381 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    13441 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
    13501 caccggctgc cggcggttg
//
