LOCUS       sequence-194536-       13552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GA biosensor expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM
  TITLE     In vivo gibberellin gradients visualized in rapidly elongating 
            tissues.
  JOURNAL   Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9.
  PUBMED    28970478
REFERENCE   2  (bases 1 to 13552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2017 Oct 2. doi: 10.1038/s41477-017-0021-9."
COMMENT     Sequence Label: p16 nlsGPS1
FEATURES             Location/Qualifiers
     source          1..13552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1322..1951
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2380..3453
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3519..3713
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3949..3971)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4057..4197
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4112..4131
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4212..4229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4383..4971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4463..4482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5217..6008)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6529..6553
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6925..7305
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             7335..8129
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7389..7408)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7999..8018
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      8325..8577
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     9144..9166
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9158..9175
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9159..9175
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(9183..9824)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(9892..9921)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(9922..10632,10633..10635))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     10313..10332
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10572..10593
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    10649..10673
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11967..11991)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(12703..12723)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(13260..13276)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13260..13276)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13273..13295)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13284..13300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13308..13314,13315..13332,13333..13338))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13353..13374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(13539..13552,1..11)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
       61 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
      121 atcaaagtac tttaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
      181 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
      241 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
      301 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
      361 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
      421 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
      481 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
      541 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
      601 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
      661 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
      721 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
      781 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
      841 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
      901 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
      961 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     1021 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     1081 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     1141 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     1201 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     1261 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     1321 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     1381 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     1441 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     1501 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     1561 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     1621 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     1681 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     1741 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     1801 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     1861 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     1921 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     1981 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     2041 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     2101 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     2161 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     2221 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     2281 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     2341 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     2401 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     2461 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     2521 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     2581 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     2641 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     2701 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     2761 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     2821 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     2881 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     2941 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     3001 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     3061 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     3121 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     3181 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     3241 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     3301 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     3361 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     3421 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     3481 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     3541 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     3601 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     3661 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     3721 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     3781 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     3841 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     3901 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     3961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     4021 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     4081 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     4141 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     4201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4321 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4801 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5041 gtcatgcatg atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata
     5101 aaagcagact tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaacgc
     5161 ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg aatttctagc tagacattat
     5221 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     5281 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     5341 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     5401 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     5461 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     5521 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     5581 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     5641 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     5701 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     5761 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     5821 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     5881 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     5941 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     6001 tcccccatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     6061 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     6121 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     6181 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     6241 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgcccgaat tgatcacagg
     6301 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
     6361 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
     6421 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
     6481 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
     6541 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
     6601 gtactgaatt aacgccgaat ttcgaggggc caacagagcc tggcgttccc cttttgcatt
     6661 gagaccgatg ttcgttccgg aaccttgcac gccccagagc ttctcaccgt tcacgacaat
     6721 ttccttctcg ttgaggtcgg tcgcgccatg tcggatgaaa taaaaacttt tgataccagc
     6781 gggggccttc gcagagccga ggtaggtctg agaaattggc attttcacgt gtggaagata
     6841 tgaatttttt tgagaaacta gataagatta atgaatatcg gtgttttggt tttttcttgt
     6901 ggccgtcttt gtttatattg agatttttca aatcagtgcg caagacgtga cgtaagtatc
     6961 cgagtcagtt tttatttttc tactaatttg gtcgtttatt tcggcgtgta ggacatggca
     7021 accgggcctg aatttcgcgg gtattctgtt tctattccaa ctttttcttg atccgcagcc
     7081 attaacgact tttgaataga tacgctgaca cgccaagcct cgctagtcaa aagtgtacca
     7141 aacaacgctt tacagcaaga acggaatgcg cgtgacgctc gcggtgacgc catttcgcct
     7201 tttcagaaat ggataaatag ccttgcttcc tattatatct tcccaaatta ccaatacatt
     7261 acactagcat ctgaatttca taaccaatct cgatacacca aatcgactct agcgaattcc
     7321 ccggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     7381 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7441 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7501 ccctgaatga actccaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     7561 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     7621 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     7681 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     7741 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     7801 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7861 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7921 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7981 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     8041 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     8101 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     8161 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     8221 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8281 gctggagttc ttcgcccacc ccggaattcc agcttccctg aaaccttgga ctcccatgtt
     8341 ggcaaaggca accaaacaaa caatgaatga tccgctcctg catatggggc ggtttgagta
     8401 tttcaactgc catttgggct gaattgaaga catgctcctg tcagaaattc cgtgatctta
     8461 ctcaatattc agtaatctcg gccaatatcc taaatgtgcg tggctttatc tgtctttgta
     8521 ttgtttcatc aattcatgta acgtttgctt ttcttatgaa ttttcaaata aattatcgat
     8581 agtactacga atatttcgta tcgctgatct tctcaatcac aatgatgcgt agtgacccga
     8641 caaataattt aagcgtcctt aataccaatc ctaaaataat tgaggcaaat aaaatttttt
     8701 tgtaattttt atgatagcag atcgattctc cagcaagcct gcaacaaaat attgtgtatt
     8761 tctaaataga ttttgatatt aaaatcccga gaaagcaaaa ttgcatttaa caaaacagta
     8821 atttagtaca ttaataaaaa ttatgctcaa acatttccat gaaattaaaa cataattaca
     8881 ttttacaaca caatattttg cgatattatt gcctttcgcc aatttaacac tggaagcaat
     8941 taaagtaggc ccacacagag cgctttgcaa cactgacaac gcttgcggtt ggtcggcctc
     9001 gattggcaag ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc
     9061 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     9121 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     9181 gcttgatgca tgttgtcaat caattggcaa gtcataaaat gcattaaaaa atattttcat
     9241 actcaactac aaatccatga gtataactat aattataaag caatgattag aatctgacaa
     9301 ggattctgga aaattacata aaggaaagtt cataaatgtc taaaacacaa gaggacatac
     9361 ttgtattcag taacatttgc agcttttcta ggtctgaaaa tatatttgtt gcctagtgaa
     9421 taagcataat ggtacaacta caagtgtttt actcctcata ttaacttcgg tcattagagg
     9481 ccacgatttg acacattttt actcaaaaca aaatgtttgc atatctctta taatttcaaa
     9541 ttcaacacac aacaaataag agaaaaaaca aataatatta atttgagaat gaacaaaagg
     9601 accatatcat tcattaactc ttctccatcc atttccattt cacagttcga tagcgaaaac
     9661 cgaataaaaa acacagtaaa ttacaagcac aacaaatggt acaagaaaaa cagttttccc
     9721 aatgccataa tactcaaact cagtaggatt ctggtgtgtg cgcaatgaaa ctgatgcatt
     9781 gaacttgacg aacgttgtcg aaaccgatga tacgaacgaa agctctgcag ccaactagtt
     9841 ggatatctcg agtcgacacg cgtagatctc cgcggacgtc ccgggccctt ataaatcttc
     9901 ctcacttatc aatttttgtt ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
     9961 ggtcaggaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgaacagggc
    10021 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
    10081 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
    10141 gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtcgctgat
    10201 ggcgttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
    10261 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
    10321 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
    10381 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcacgcccca
    10441 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttaccggtgg tgcagatgaa
    10501 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
    10561 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
    10621 ctcgcccttg ctcacactag tcgatatcac cactttgtac aagaaagctg ggtgttggca
    10681 ttctgcgttt acaaatgcag ctatctcatc cataacggta tggaagtgat tgttgttagg
    10741 caataagtag aagccaatag tggcttgctc caagtaaaga agcttcacct cttgacccgc
    10801 tttcttgagc ccttccgcgt acttcaattg ccaatcttga atcaaatcta aacccgctac
    10861 aacgacaaga ctcttgggga aactcaaccc ttctagactc ttgcttctcg ggccaaacgg
    10921 gctacacgct ggatgctctc tgtcttcacc ctcaggaaga aacgctctcc aataccaatc
    10981 tcggtctcta accgtaacaa agtacttccc atccaaacgt ttctcagatt ccgttctttc
    11041 ggtccctcca aacatagggt taagcaaaat gttccccaaa acatcgatcc ttgactcaac
    11101 cgctcttacc gcgacattat gcacaatgtt acccccagag ctatcacccg ccaagaaaat
    11161 acgaaccttt gagtctttct tgcttctaag ccaagaactc gagttgaccc atttcaaaac
    11221 agcccatcca tcatcataag cacaagggta tcgattctct ggtgcacgac gataattcac
    11281 agagacaaca acagcaccac acaaaccaac aagcctacga caaagagtat cataaatagc
    11341 actgtttgca gaagaatgtg caaagcttcc accatgaaag aagacaataa caggcactat
    11401 ttcaccatca acaggattct gtagatcagt aatacttggt gaagtgccag catcagctgg
    11461 tctgtaaact ctgctaagca aattagtttg gcgatcgatg atcacatcaa aagagaagac
    11521 cccattaaca gggttggcat ttgcagggac tttccggtct agaaactctg cgagatgacg
    11581 gttaaaagtt ccgtcagggc gacgcaggag attgtaagct agcttaaagt tggatattag
    11641 aacccatgta ttgagaggaa ccactgtctt gctctcaata agattaactt cttcacttcc
    11701 agccattcca gaacttcggc cggccccagg cgcgccactt gcatcgtact cggcgttaga
    11761 cgacggagga ttaaggtcgg tgagcataga atcaagccac gtgtaaagct ccgccggatt
    11821 atagtgaaca gtctcagtag cgagttgaga aagatcgtct tcttgaacat tagacatcat
    11881 aacttcaagc tgctcgagtt tctgagcaac atcagccatt tcggatgacc taaccttgta
    11941 accaagaaca gctagaagct catcgaagcc tgcttttttg tacaaacttg tgatatcggt
    12001 acctatgccc gccgccgtga ggaactccag gagaaccatg tggtcccgtt tctcattcgg
    12061 gtccttgaag agcgcgcttt ggtacgagag gtaatggtta tccgggagga ggaccggccc
    12121 atcccctatc ggcgtgtttt gctgatagtg atccgccagt tggacccccc catcctctat
    12181 gttatgccgg attttgaaat tggctttgat cccgttcttt tgcttatccg cggtgatgta
    12241 tacgttatgc gagttgtaat tgtattcgag cttatggccg agtatgttcc catcctcttt
    12301 gaagtcaatc cccttgagtt cgatccggtt gacgagcgtg tccccttcaa acttgacttc
    12361 cgcccgcgtc ttatagttcc catcgtcttt gaaaaagatc gtccgttcct gcacgtatcc
    12421 ctccggcatc gccgatttga aaaagtcatg ctgtttcatg tggtccggat acctcgcaaa
    12481 gcattggagc ccgtatccca gcgtcgtgac cagcgtcggc cacgggaccg ggagtttccc
    12541 cgtcgtacag atcagcttga gagtgagctt cccatacgtc gcgtccccct ccccttcccc
    12601 gctgaccgaa aacttatgcc cgttaacatc cccgtcgagt tccacgagga tcgggacgac
    12661 gcccgtaaac aattcttcac ctttagaaac ctcgagccct ccaacctttc tcttcttctt
    12721 aggctgcagc atcatgagct ctagaggatc cccgggtacc ccacgccgtc gtagatgaga
    12781 aaccctaatg agcaagttcg cttgtcacat taagcgtctt tatatacgaa tcgaatcatg
    12841 ttagggcatt ttttaatatg ggcctttgaa ctatcatata tgggccatca aatcaaccat
    12901 aaagacataa aagccttgtt gctcatatga agtgcagact ttttaatatg gccctttaac
    12961 ctcatgtggt aatataactc cgatttgatt gaatttaatc aaaatttatt aataacgata
    13021 ttggaagaac attgagctct ctgtgcttta aaactgatgt acgaaaaggt taaacacaga
    13081 aatgagaaat tttggaagtt ttttgctata taataaaata gagaaactcc ctttataaca
    13141 aattagtttt gtttgaagca cttgaaaaga gaaaagactt ttaagagaga aacaaaaaaa
    13201 gagaagaaga aaggaaccca aaagatggtt ccaggtaccg agctcgaatt cgtaatcatg
    13261 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    13321 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    13381 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    13441 ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgag cttggatcag
    13501 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata tt
//
