LOCUS       sequence-194537-       13552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GA biosensor expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM
  TITLE     In vivo gibberellin gradients visualized in rapidly elongating 
            tissues.
  JOURNAL   Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9.
  PUBMED    28970478
REFERENCE   2  (bases 1 to 13552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2017 Oct 2. doi: 10.1038/s41477-017-0021-9."
COMMENT     Sequence Label: p16 nlsGPS1-NR
FEATURES             Location/Qualifiers
     source          1..13552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             56..1129
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1195..1389
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1625..1647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1733..1873
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1788..1807
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1888..1905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2059..2647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2139..2158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2893..3684)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    4205..4229
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4601..4981
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             5011..5805
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5065..5084)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5675..5694
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6001..6253
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     6820..6842
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6834..6851
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6835..6851
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6859..7500)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(7568..7597)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(7598..8308,8309..8311))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     7989..8008
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8248..8269
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    8325..8349
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9643..9667)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(10379..10399)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10936..10952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10936..10952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10949..10971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10960..10976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10984..10990,10991..11008,11009..11014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11029..11050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    11215..11239
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12550..13179
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
       61 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
      121 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
      181 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
      241 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
      301 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
      361 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
      421 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
      481 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
      541 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
      601 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
      661 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
      721 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
      781 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
      841 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
      901 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
      961 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     1021 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     1081 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     1141 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     1201 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     1261 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     1321 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     1381 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     1441 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     1501 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     1561 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     1621 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1681 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1741 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1801 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     1861 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2701 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
     2761 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
     2821 ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt
     2881 tctagctaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     2941 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     3001 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     3061 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     3121 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     3181 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     3241 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     3301 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     3361 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     3421 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     3481 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     3541 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     3601 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     3661 ttcggcgatc accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg
     3721 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     3781 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
     3841 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     3901 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
     3961 ccgaattgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     4021 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     4081 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     4141 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     4201 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     4261 tgcggacgtt tttaatgtac tgaattaacg ccgaatttcg aggggccaac agagcctggc
     4321 gttccccttt tgcattgaga ccgatgttcg ttccggaacc ttgcacgccc cagagcttct
     4381 caccgttcac gacaatttcc ttctcgttga ggtcggtcgc gccatgtcgg atgaaataaa
     4441 aacttttgat accagcgggg gccttcgcag agccgaggta ggtctgagaa attggcattt
     4501 tcacgtgtgg aagatatgaa tttttttgag aaactagata agattaatga atatcggtgt
     4561 tttggttttt tcttgtggcc gtctttgttt atattgagat ttttcaaatc agtgcgcaag
     4621 acgtgacgta agtatccgag tcagttttta tttttctact aatttggtcg tttatttcgg
     4681 cgtgtaggac atggcaaccg ggcctgaatt tcgcgggtat tctgtttcta ttccaacttt
     4741 ttcttgatcc gcagccatta acgacttttg aatagatacg ctgacacgcc aagcctcgct
     4801 agtcaaaagt gtaccaaaca acgctttaca gcaagaacgg aatgcgcgtg acgctcgcgg
     4861 tgacgccatt tcgccttttc agaaatggat aaatagcctt gcttcctatt atatcttccc
     4921 aaattaccaa tacattacac tagcatctga atttcataac caatctcgat acaccaaatc
     4981 gactctagcg aattccccgg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     5041 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5101 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5161 accgacctgt ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg
     5221 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5281 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5341 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5401 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5461 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5521 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5581 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5641 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5701 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5761 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5821 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5881 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5941 agcgcgggga tctcatgctg gagttcttcg cccaccccgg aattccagct tccctgaaac
     6001 cttggactcc catgttggca aaggcaacca aacaaacaat gaatgatccg ctcctgcata
     6061 tggggcggtt tgagtatttc aactgccatt tgggctgaat tgaagacatg ctcctgtcag
     6121 aaattccgtg atcttactca atattcagta atctcggcca atatcctaaa tgtgcgtggc
     6181 tttatctgtc tttgtattgt ttcatcaatt catgtaacgt ttgcttttct tatgaatttt
     6241 caaataaatt atcgatagta ctacgaatat ttcgtatcgc tgatcttctc aatcacaatg
     6301 atgcgtagtg acccgacaaa taatttaagc gtccttaata ccaatcctaa aataattgag
     6361 gcaaataaaa tttttttgta atttttatga tagcagatcg attctccagc aagcctgcaa
     6421 caaaatattg tgtatttcta aatagatttt gatattaaaa tcccgagaaa gcaaaattgc
     6481 atttaacaaa acagtaattt agtacattaa taaaaattat gctcaaacat ttccatgaaa
     6541 ttaaaacata attacatttt acaacacaat attttgcgat attattgcct ttcgccaatt
     6601 taacactgga agcaattaaa gtaggcccac acagagcgct ttgcaacact gacaacgctt
     6661 gcggttggtc ggcctcgatt ggcaagctgc tctagcattc gccattcagg ctgcgcaact
     6721 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     6781 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     6841 cgacggccag tgccaagctt gatgcatgtt gtcaatcaat tggcaagtca taaaatgcat
     6901 taaaaaatat tttcatactc aactacaaat ccatgagtat aactataatt ataaagcaat
     6961 gattagaatc tgacaaggat tctggaaaat tacataaagg aaagttcata aatgtctaaa
     7021 acacaagagg acatacttgt attcagtaac atttgcagct tttctaggtc tgaaaatata
     7081 tttgttgcct agtgaataag cataatggta caactacaag tgttttactc ctcatattaa
     7141 cttcggtcat tagaggccac gatttgacac atttttactc aaaacaaaat gtttgcatat
     7201 ctcttataat ttcaaattca acacacaaca aataagagaa aaaacaaata atattaattt
     7261 gagaatgaac aaaaggacca tatcattcat taactcttct ccatccattt ccatttcaca
     7321 gttcgatagc gaaaaccgaa taaaaaacac agtaaattac aagcacaaca aatggtacaa
     7381 gaaaaacagt tttcccaatg ccataatact caaactcagt aggattctgg tgtgtgcgca
     7441 atgaaactga tgcattgaac ttgacgaacg ttgtcgaaac cgatgatacg aacgaaagct
     7501 ctgcagccaa ctagttggat atctcgagtc gacacgcgta gatctccgcg gacgtcccgg
     7561 gcccttataa atcttcctca cttatcaatt tttgttcctt gtacagctcg tccatgccga
     7621 gagtgatccc ggcggcggtc aggaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     7681 ggtctttgaa cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     7741 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     7801 tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat
     7861 agacgttgtc gctgatggcg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     7921 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     7981 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     8041 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
     8101 agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttac
     8161 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     8221 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     8281 ccccggtgaa cagctcctcg cccttgctca cactagtcga tatcaccact ttgtacaaga
     8341 aagctgggtg ttggcattct gcgtttacaa atgcagctat ctcatccata acggtatgga
     8401 agtgattgtt gttaggcaat aagtagaagc caatagtggc ttgctccaag taaagaagct
     8461 tcacctcttg acccgctttc ttgagccctt ccgcgtactt caattgccaa tcttgaatca
     8521 aatctaaacc cgctacaacg acaagactct tggggaaact caacccttct agactcttgc
     8581 ttctcgggcc aaacgggcta cacgctggat gctctctgtc ttcaccctca ggaagaaacg
     8641 ctctccaata ccaatctcgg tctctaaccg taacaaagta cttcccatcc aaacgtttct
     8701 cagattccgt tctttcggtc cctccaaaca tagggttaag caaaatgttc cccaaaacat
     8761 cgatccttga ctcaaccgct cttaccgcga cattatgcac aatgttaccc ccagagctat
     8821 cacccgccaa gaaaatacga acctttgagt ctttcttgct tctaagccaa gaactcgagt
     8881 tgacccattt caaaacagcc catccatcat cataagcaca agggtatcga ttctctggtg
     8941 cacgacgata attcacagag acaacaacag caccacacaa accaacaagc ctacgacaaa
     9001 gagtatcata aatagcactg tttgcagaag aatgtgcagc ggctccacca tgaaagaaga
     9061 caataacagg cactatttca ccatcaacag gattctgtag atcagtaata cttggtgaag
     9121 tgccagcatc agctggtctg taaactctgc taagcaaatt agtttggcga tcgatgatca
     9181 catcaaaaga gaagacccca ttaacagggt tggcatttgc agggactttc cggtctagaa
     9241 actctgcgag atgacggtta aaagttccgt cagggcgacg caggagattg taagctagct
     9301 taaagttgga tattagaacc catgtattga gaggaaccac tgtcttgctc tcaataagat
     9361 taacttcttc acttccagcc attccagaac ttcggccggc cccaggcgcg ccacttgcat
     9421 cgtactcggc gttagacgac ggaggattaa ggtcggtgag catagaatca agccacgtgt
     9481 aaagctccgc cggattatag tgaacagtct cagtagcgag ttgagaaaga tcgtcttctt
     9541 gaacattaga catcataact ctaagctgct tgagtttctg agcaacatca gccatttcgg
     9601 atgacctaac cttgtaacca agaacagcta gaagctcatc gaagcctgct tttttgtaca
     9661 aacttgtgat atcggtacct atgcccgccg ccgtgaggaa ctccaggaga accatgtggt
     9721 cccgtttctc attcgggtcc ttgaagagcg cgctttggta cgagaggtaa tggttatccg
     9781 ggaggaggac cggcccatcc cctatcggcg tgttttgctg atagtgatcc gccagttgga
     9841 cccccccatc ctctatgtta tgccggattt tgaaattggc tttgatcccg ttcttttgct
     9901 tatccgcggt gatgtatacg ttatgcgagt tgtaattgta ttcgagctta tggccgagta
     9961 tgttcccatc ctctttgaag tcaatcccct tgagttcgat ccggttgacg agcgtgtccc
    10021 cttcaaactt gacttccgcc cgcgtcttat agttcccatc gtctttgaaa aagatcgtcc
    10081 gttcctgcac gtatccctcc ggcatcgccg atttgaaaaa gtcatgctgt ttcatgtggt
    10141 ccggatacct cgcaaagcat tggagcccgt atcccagcgt cgtgaccagc gtcggccacg
    10201 ggaccgggag tttccccgtc gtacagatca gcttgagagt gagcttccca tacgtcgcgt
    10261 ccccctcccc ttccccgctg accgaaaact tatgcccgtt aacatccccg tcgagttcca
    10321 cgaggatcgg gacgacgccc gtaaacaatt cttcaccttt agaaacctcg agccctccaa
    10381 cctttctctt cttcttaggc tgcagcatca tgagctctag aggatccccg ggtaccccac
    10441 gccgtcgtag atgagaaacc ctaatgagca agttcgcttg tcacattaag cgtctttata
    10501 tacgaatcga atcatgttag ggcatttttt aatatgggcc tttgaactat catatatggg
    10561 ccatcaaatc aaccataaag acataaaagc cttgttgctc atatgaagtg cagacttttt
    10621 aatatggccc tttaacctca tgtggtaata taactccgat ttgattgaat ttaatcaaaa
    10681 tttattaata acgatattgg aagaacattg agctctctgt gctttaaaac tgatgtacga
    10741 aaaggttaaa cacagaaatg agaaattttg gaagtttttt gctatataat aaaatagaga
    10801 aactcccttt ataacaaatt agttttgttt gaagcacttg aaaagagaaa agacttttaa
    10861 gagagaaaca aaaaaagaga agaagaaagg aacccaaaag atggttccag gtaccgagct
    10921 cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10981 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    11041 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    11101 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctagagcag
    11161 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
    11221 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
    11281 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
    11341 ccctcgggat caaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag
    11401 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
    11461 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
    11521 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
    11581 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
    11641 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
    11701 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
    11761 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
    11821 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
    11881 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
    11941 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
    12001 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
    12061 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
    12121 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
    12181 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
    12241 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
    12301 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
    12361 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
    12421 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
    12481 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
    12541 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
    12601 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
    12661 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
    12721 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
    12781 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
    12841 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
    12901 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
    12961 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
    13021 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
    13081 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
    13141 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
    13201 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
    13261 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
    13321 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
    13381 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
    13441 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
    13501 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ct
//
