LOCUS       sequence-194538-        9349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GA biosensor expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM
  TITLE     In vivo gibberellin gradients visualized in rapidly elongating 
            tissues.
  JOURNAL   Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9.
  PUBMED    28970478
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2017 Oct 2. doi: 10.1038/s41477-017-0021-9."
COMMENT     Sequence Label: pDR FLIP43 GPS1
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(213..232)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             624..653
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(691..707)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..708)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(732..751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(733..751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      899..1086
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(954..978)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1098..1115)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1098..1114)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1107..1129)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1550..2896
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3007..3810)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3811..4026)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4051..4069)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4137..4241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4242..4310,4311..5102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4460..4479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5273..5861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5762..5781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6015..6032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6149..6170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6185..6190,6191..6208,6209..6215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6223..6239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6228..6250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6247..6263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6247..6263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             7066..7088
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7108..7125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7129..7161
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(7165..7173,7174..7188)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    7903..7927
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9221..9245)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9259..9261,9262..9349,1..623)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     complement(9301..9322)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
       61 gtaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct
      121 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      181 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      241 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      301 aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatcagc gacaacgtct
      361 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
      421 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      481 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg ttcaaagacc
      541 ccaacgagaa gcgcgatcac atggtcctgc tggagttcct gaccgccgcc gggatcactc
      601 tcggcatgga cgagctgtac aaggaacaaa aattgataag tgaggaagat ttataagggc
      661 cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
      721 tacccaattc gccctatagt gagtcgtatt acgcgcggat ccagctttgg acttcttcgc
      781 cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta
      841 cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc
      901 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
      961 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
     1021 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc
     1081 taccggcatg ccaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1141 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1201 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     1261 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataat cggatcgtac
     1321 ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg aaacagatag
     1381 tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat
     1441 ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc
     1501 ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc
     1561 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     1621 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
     1681 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     1741 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa
     1801 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct
     1861 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga
     1921 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc
     1981 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg
     2041 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc
     2101 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa
     2161 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg
     2221 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
     2281 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
     2341 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
     2401 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
     2461 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
     2521 tatactttct agctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa
     2581 cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac
     2641 ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt
     2701 atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct
     2761 cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta
     2821 gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt
     2881 cctttgatat tggatcgatc cgatgataag ctgtcaaaca tgagaattgg gtaataactg
     2941 atataattaa attgaagctc taatttgtga gtttagtata catgcattta cttataatac
     3001 agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta
     3061 acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat
     3121 aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc
     3181 tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct
     3241 tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat
     3301 gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg acaattctgc
     3361 taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct
     3421 aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct
     3481 gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc
     3541 ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc
     3601 catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa
     3661 tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt
     3721 tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc
     3781 agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc atgtttttgt
     3841 tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg
     3901 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
     3961 cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
     4021 attgaaaagc taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4081 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4141 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4201 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4261 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4321 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4381 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4441 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4501 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4561 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4621 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4681 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4741 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4801 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4861 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4921 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4981 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5041 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5101 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5161 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5401 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5641 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5941 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6001 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6061 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6121 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6181 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6241 ttcacacagg aaacagctat gaccatgatt acgccaagct tcctgaaacg gagaaacata
     6301 aacaggcatt gctgggatca cccatacatc actctgtttt gcctgacctt ttccggtaat
     6361 ttgaaaacaa acccggtctc gaagcggaga tccggcgata attaccgcag aaataaaccc
     6421 atacacgaga cgtagaacca gccgcacatg gccggagaaa ctcctgcgag aatttcgtaa
     6481 actcgcgcgc attgcatctg tatttcctaa tgcggcactt ccaggcctcg atcgagaccg
     6541 tttatccatt gcttttttgt tgtctttttc cctcgttcac agaaagtctg aagaagctat
     6601 agtagaacta tgagcttttt ttgtttctgt tttccttttt ttttttttta cctctgtgga
     6661 aattgttact ctcacactct ttagttcgtt tgtttgtttt gtttattcca attatgaccg
     6721 gtgacgaaac gtggtcgatg gtgggtaccg cttatgctcc cctccattag tttcgattat
     6781 ataaaaaggc caaatattgt attattttca aatgtcctat cattatcgtc taacatctaa
     6841 tttctcttaa attttttctc tttctttcct ataacaccaa tagtgaaaat ctttttttct
     6901 tctatatcta caaaaacttt ttttttctat caacctcgtt gataaatttt ttctttaaca
     6961 atcgttaata attaattaat tggaaaataa ccattttttc tctcttttat acacacattc
     7021 aaaagaaaga aaaaaaatat accccagcct cgatctagaa ataattttgt ttaactttaa
     7081 gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat ggctagcatg
     7141 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgggcctc
     7201 gaggtttcta aaggtgaaga attgtttacg ggcgtcgtcc cgatcctcgt ggaactcgac
     7261 ggggatgtta acgggcataa gttttcggtc agcggggaag gggaggggga cgcgacgtat
     7321 gggaagctca ctctcaagct gatctgtacg acggggaaac tcccggtccc gtggccgacg
     7381 ctggtcacga cgctgggata cgggctccaa tgctttgcga ggtatccgga ccacatgaaa
     7441 cagcatgact ttttcaaatc ggcgatgccg gagggatacg tgcaggaacg gacgatcttt
     7501 ttcaaagacg atgggaacta taagacgcgg gcggaagtca agtttgaagg ggacacgctc
     7561 gtcaaccgga tcgaactcaa ggggattgac ttcaaagagg atgggaacat actcggccat
     7621 aagctcgaat acaattacaa ctcgcataac gtatacatca ccgcggataa gcaaaagaac
     7681 gggatcaaag ccaatttcaa aatccggcat aacatagagg atgggggggt ccaactggcg
     7741 gatcactatc agcaaaacac gccgataggg gatgggccgg tcctcctccc ggataaccat
     7801 tacctctcgt accaaagcgc gctcttcaag gacccgaatg agaaacggga ccacatggtt
     7861 ctcctggagt tcctcacggc ggcgggcata ggtaccgata tcacaagttt gtacaaaaaa
     7921 gcaggcttcg atgagcttct agctgttctt ggttacaagg ttaggtcatc cgaaatggct
     7981 gatgttgctc agaaactcga gcagcttgaa gttatgatgt ctaatgttca agaagacgat
     8041 ctttctcaac tcgctactga gactgttcac tataatccgg cggagcttta cacgtggctt
     8101 gattctatgc tcaccgacct taatcctccg tcgtctaacg ccgagtacga tgcaagtggc
     8161 gcgcctgggg ccggccgaag ttctggaatg gctggaagtg aagaagttaa tcttattgag
     8221 agcaagacag tggttcctct caatacatgg gttctaatat ccaactttaa gctagcttac
     8281 aatctcctgc gtcgccctga cggaactttt aaccgtcatc tcgcagagtt tctagaccgg
     8341 aaagtccctg caaatgccaa ccctgttaat ggggtcttct cttttgatgt gatcatcgat
     8401 cgccaaacta atttgcttag cagagtttac agaccagctg atgctggcac ttcaccaagt
     8461 attactgatc tacagaatcc tgttgatggt gaaatagtgc ctgttattgt cttctttcat
     8521 ggtggaagct ttgcacattc ttctgcaaac agtgctattt atgatactct ttgtcgtagg
     8581 cttgttggtt tgtgtggtgc tgttgttgtc tctgtgaatt atcgtcgtgc accagagaat
     8641 cgataccctt gtgcttatga tgatggatgg gctgttttga aatgggtcaa ctcgagttct
     8701 tggcttagaa gcaagaaaga ctcaaaggtt cgtattttct tggcgggtga tagctctggg
     8761 ggtaacattg tgcataatgt cgcggtaaga gcggttgagt caaggatcga tgttttgggg
     8821 aacattttgc ttaaccctat gtttggaggg accgaaagaa cggaatctga gaaacgtttg
     8881 gatgggaagt actttgttac ggttagagac cgagattggt attggagagc gtttcttcct
     8941 gagggtgaag acagagagca tccagcgtgt agcccgtttg gcccgagaag caagagtcta
     9001 gaagggttga gtttccccaa gagtcttgtc gttgtagcgg gtttagattt gattcaagat
     9061 tggcaattga agtacgcgga agggctcaag aaagcgggtc aagaggtgaa gcttctttac
     9121 ttggagcaag ccactattgg cttctactta ttgcctaaca acaatcactt ccataccgtt
     9181 atggatgaga tagctgcatt tgtaaacgca gaatgccaac acccagcttt cttgtacaaa
     9241 gtggtgatat cgactagtgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     9301 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccg
//
