LOCUS       sequence-194539-        9349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GA biosensor expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM
  TITLE     In vivo gibberellin gradients visualized in rapidly elongating 
            tissues.
  JOURNAL   Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9.
  PUBMED    28970478
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2017 Oct 2. doi: 10.1038/s41477-017-0021-9."
COMMENT     Sequence Label: pDR FLIP43 GPS1-NR
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1061..1083
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1075..1092
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1076..1092
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1104..1291)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     1212..1236
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     1439..1458
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1439..1457
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1482..1498
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1483..1499
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1537..1566)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(1567..2277,2278..2280))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     1958..1977
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2217..2238
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    2294..2318
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3612..3636)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4351..4365,4366..4374))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(4378..4410)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(4414..4431)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             4451..4473
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(5276..5292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5276..5292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5289..5311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5300..5316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5324..5330,5331..5348,5349..5354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5369..5390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5507..5524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5678..6266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5758..5777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6437..7228,7229..7297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7060..7079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7298..7402)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7470..7488
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7513..7728
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             7729..8532
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      join(8643..9349,1..640)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc
       61 aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg
      121 gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg
      181 aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa
      241 ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg
      301 agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg
      361 tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc
      421 tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat
      481 ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc
      541 atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt
      601 tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa
      661 gtgcaagatg gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa
      721 tagatgcaat agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga
      781 ctatatatta ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg
      841 gatgttcaaa attcaatgat gggtaacaag tacgatccga ttatgcggtg tgaaataccg
      901 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
      961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     1021 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     1081 acgacggcca gtgaattggc atgccggtag aggtgtggtc aataagagcg acctcatgct
     1141 atacctgaga aagcaacctg acctacagga aagagttact caagaataag aattttcgtt
     1201 ttaaaaccta agagtcactt taaaatttgt atacacttat tttttttata acttatttaa
     1261 taataaaaat cataaatcat aagaaattcg cttatttaga agtgtcaaca acgtatctac
     1321 caacgatttg acccttttcc atcttttcgt aaatttctgg caaggtagac aagccgacaa
     1381 ccttgattgg agacttgacc aaacctctgg cgaagaagtc caaagctgga tccgcgcgta
     1441 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga
     1501 taagcttgat atcgaattcc tgcagcccgg cccttataaa tcttcctcac ttatcaattt
     1561 ttgttccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca ggaactccag
     1621 caggaccatg tgatcgcgct tctcgttggg gtctttgaac agggcggact gggtgctcag
     1681 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     1741 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat
     1801 gccgttcttc tgcttgtcgg cggtgatata gacgttgtcg ctgatggcgt tgtactccag
     1861 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     1921 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     1981 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     2041 ctgcttcatg tggtcggggt agcgggcgaa gcactgcacg ccccaggtca gggtggtcac
     2101 gagggtgggc cagggcacgg gcagcttacc ggtggtgcag atgaacttca gggtcagctt
     2161 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     2221 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     2281 actagtcgat atcaccactt tgtacaagaa agctgggtgt tggcattctg cgtttacaaa
     2341 tgcagctatc tcatccataa cggtatggaa gtgattgttg ttaggcaata agtagaagcc
     2401 aatagtggct tgctccaagt aaagaagctt cacctcttga cccgctttct tgagcccttc
     2461 cgcgtacttc aattgccaat cttgaatcaa atctaaaccc gctacaacga caagactctt
     2521 ggggaaactc aacccttcta gactcttgct tctcgggcca aacgggctac acgctggatg
     2581 ctctctgtct tcaccctcag gaagaaacgc tctccaatac caatctcggt ctctaaccgt
     2641 aacaaagtac ttcccatcca aacgtttctc agattccgtt ctttcggtcc ctccaaacat
     2701 agggttaagc aaaatgttcc ccaaaacatc gatccttgac tcaaccgctc ttaccgcgac
     2761 attatgcaca atgttacccc cagagctatc acccgccaag aaaatacgaa cctttgagtc
     2821 tttcttgctt ctaagccaag aactcgagtt gacccatttc aaaacagccc atccatcatc
     2881 ataagcacaa gggtatcgat tctctggtgc acgacgataa ttcacagaga caacaacagc
     2941 accacacaaa ccaacaagcc tacgacaaag agtatcataa atagcactgt ttgcagaaga
     3001 atgtgcagcg gctccaccat gaaagaagac aataacaggc actatttcac catcaacagg
     3061 attctgtaga tcagtaatac ttggtgaagt gccagcatca gctggtctgt aaactctgct
     3121 aagcaaatta gtttggcgat cgatgatcac atcaaaagag aagaccccat taacagggtt
     3181 ggcatttgca gggactttcc ggtctagaaa ctctgcgaga tgacggttaa aagttccgtc
     3241 agggcgacgc aggagattgt aagctagctt aaagttggat attagaaccc atgtattgag
     3301 aggaaccact gtcttgctct caataagatt aacttcttca cttccagcca ttccagaact
     3361 tcggccggcc ccaggcgcgc cacttgcatc gtactcggcg ttagacgacg gaggattaag
     3421 gtcggtgagc atagaatcaa gccacgtgta aagctccgcc ggattatagt gaacagtctc
     3481 agtagcgagt tgagaaagat cgtcttcttg aacattagac atcataactc taagctgctt
     3541 gagtttctga gcaacatcag ccatttcgga tgacctaacc ttgtaaccaa gaacagctag
     3601 aagctcatcg aagcctgctt ttttgtacaa acttgtgata tcggtaccta tgcccgccgc
     3661 cgtgaggaac tccaggagaa ccatgtggtc ccgtttctca ttcgggtcct tgaagagcgc
     3721 gctttggtac gagaggtaat ggttatccgg gaggaggacc ggcccatccc ctatcggcgt
     3781 gttttgctga tagtgatccg ccagttggac ccccccatcc tctatgttat gccggatttt
     3841 gaaattggct ttgatcccgt tcttttgctt atccgcggtg atgtatacgt tatgcgagtt
     3901 gtaattgtat tcgagcttat ggccgagtat gttcccatcc tctttgaagt caatcccctt
     3961 gagttcgatc cggttgacga gcgtgtcccc ttcaaacttg acttccgccc gcgtcttata
     4021 gttcccatcg tctttgaaaa agatcgtccg ttcctgcacg tatccctccg gcatcgccga
     4081 tttgaaaaag tcatgctgtt tcatgtggtc cggatacctc gcaaagcatt ggagcccgta
     4141 tcccagcgtc gtgaccagcg tcggccacgg gaccgggagt ttccccgtcg tacagatcag
     4201 cttgagagtg agcttcccat acgtcgcgtc cccctcccct tccccgctga ccgaaaactt
     4261 atgcccgtta acatccccgt cgagttccac gaggatcggg acgacgcccg taaacaattc
     4321 ttcaccttta gaaacctcga ggcccggatc cttatcgtca tcgtcgtaca gatcccgacc
     4381 catttgctgt ccaccagtca tgctagccat accatgatga tgatgatgat gagaaccccg
     4441 catatgtata tctccttctt aaagttaaac aaaattattt ctagatcgag gctggggtat
     4501 attttttttc tttcttttga atgtgtgtat aaaagagaga aaaaatggtt attttccaat
     4561 taattaatta ttaacgattg ttaaagaaaa aatttatcaa cgaggttgat agaaaaaaaa
     4621 agtttttgta gatatagaag aaaaaaagat tttcactatt ggtgttatag gaaagaaaga
     4681 gaaaaaattt aagagaaatt agatgttaga cgataatgat aggacatttg aaaataatac
     4741 aatatttggc ctttttatat aatcgaaact aatggagggg agcataagcg gtacccacca
     4801 tcgaccacgt ttcgtcaccg gtcataattg gaataaacaa aacaaacaaa cgaactaaag
     4861 agtgtgagag taacaatttc cacagaggta aaaaaaaaaa aaaggaaaac agaaacaaaa
     4921 aaagctcata gttctactat agcttcttca gactttctgt gaacgaggga aaaagacaac
     4981 aaaaaagcaa tggataaacg gtctcgatcg aggcctggaa gtgccgcatt aggaaataca
     5041 gatgcaatgc gcgcgagttt acgaaattct cgcaggagtt tctccggcca tgtgcggctg
     5101 gttctacgtc tcgtgtatgg gtttatttct gcggtaatta tcgccggatc tccgcttcga
     5161 gaccgggttt gttttcaaat taccggaaaa ggtcaggcaa aacagagtga tgtatgggtg
     5221 atcccagcaa tgcctgttta tgtttctccg tttcaggaag cttggcgtaa tcatggtcat
     5281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     7501 aagaattagc ttttcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct
     7561 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     7621 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     7681 ttaacccaac tgcacagaac aaaaacatgc aggaaacgaa gataaatcat gtcgaaagct
     7741 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     7801 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     7861 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     7921 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     7981 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
     8041 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     8101 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     8161 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     8221 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     8281 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     8341 ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc
     8401 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     8461 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     8521 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     8581 gcttcaattt aattatatca gttattaccc aattctcatg tttgacagct tatcatcgga
     8641 tcgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca attgaggagt
     8701 ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac cccgcatgga
     8761 atgggataat atcacaggag gtactagact acctttcatc ctacataaat agacgcatat
     8821 aagtacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca
     8881 ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg
     8941 cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt
     9001 ctctagctag aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga
     9061 cgcactttca aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat
     9121 accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc
     9181 ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac
     9241 attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt
     9301 catagagtga atcgaaaaca atacgaaaat gtaaacattt cctatacgt
//
