LOCUS       sequence-194555-       16900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The dCas9-TET1CD fusion protein is cloned into the pINDUCER 
            lentiviral backbone (Addgene plasmid# 46948)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 16900)
  AUTHORS   Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V,
            Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, 
            Huangfu D
  TITLE     TET proteins safeguard bivalent promoters from de novo methylation 
            in human embryonic stem cells.
  JOURNAL   Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. 
            Epub 2017 Dec 4.
  PUBMED    29203910
REFERENCE   2  (bases 1 to 16900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-017-0002-y"; journalName: "Nat Genet"; date: 
            "2018-01"; volume: "50"; issue: "1"; pages: "83-95"
COMMENT     Sequence Label: pINDUCER dCas9-TET1CD
FEATURES             Location/Qualifiers
     source          1..16900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..56
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     53..77
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    186..210
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      235..244
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             244..309
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             316..336
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(361..384,385..387,388..2874,2875..2877,2878..4461)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             361..4461
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(6702..6726)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6779..6805
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        7025..8203
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7256..8194
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8151..8171
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8273..8306
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(8313..8930,8931..8936,8937..8972,8973..8975,8976..9011,
                     9012..9014,9015..9050,9051..9059)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9219..9681
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9275..9292)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9502..9521
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9697..9699,9700..9702,9703..10416)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9697..9699,9700..9702,9703..10413)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10003..10022)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10350..10371
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10552..10571
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    10859..11447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10912..10932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11330..11341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11522..11755
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11874..11892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11960..12064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12065..12133,12134..12925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12283..12302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13096..13684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13585..13604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13838..13855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14095..14116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14131..14136,14137..14154,14155..14161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14169..14185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14174..14196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14193..14209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14193..14209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14259..14278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             14470..15103
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    15150..15275
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    15772..16005
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             16190..16234
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    16527..16644
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    16699..16717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16741..16759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16783..16801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16825..16843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16867..16885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
       61 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      121 accgggaccg atccagcctc cgcggccccg aactagtcca gtgtggtgga attctgcaga
      181 tatcaacaag tttgtacaaa aaagcaggct ccgcggtttt caggttggac cggtgccacc
      241 atggactata aggaccacga cggagactac aaggatcatg atattgatta caaagacgat
      301 gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc
      361 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
      421 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
      481 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
      541 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
      601 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
      661 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
      721 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
      781 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
      841 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
      901 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
      961 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     1021 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     1081 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     1141 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     1201 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     1261 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     1321 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     1381 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     1441 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     1501 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     1561 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     1621 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     1681 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     1741 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     1801 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     1861 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     1921 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     1981 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     2041 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     2101 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     2161 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     2221 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     2281 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     2341 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     2401 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     2461 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     2521 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     2581 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     2641 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     2701 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     2761 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     2821 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc
     2881 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     2941 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     3001 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     3061 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     3121 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     3181 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     3241 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     3301 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     3361 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     3421 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     3481 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     3541 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     3601 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     3661 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     3721 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     3781 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     3841 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     3901 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     3961 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     4021 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     4081 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     4141 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     4201 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     4261 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     4321 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     4381 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     4441 ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccagctgccc
     4501 acctgcagct gtcttgatcg agttatacaa aaagacaaag gcccatatta tacacacctt
     4561 ggggcaggac caagtgttgc tgctgtcagg gaaatcatgg agaataggta tggtcaaaaa
     4621 ggaaacgcaa taaggataga aatagtagtg tacaccggta aagaagggaa aagctctcat
     4681 gggtgtccaa ttgctaagtg ggttttaaga agaagcagtg atgaagaaaa agttctttgt
     4741 ttggtccggc agcgtacagg ccaccactgt ccaactgctg tgatggtggt gctcatcatg
     4801 gtgtgggatg gcatccctct tccaatggcc gaccggctat acacagagct cacagagaat
     4861 ctaaagtcat acaatgggca ccctaccgac agaagatgca ccctcaatga aaatcgtacc
     4921 tgtacatgtc aaggaattga tccagagact tgtggagctt cattctcttt tggctgttca
     4981 tggagtatgt actttaatgg ctgtaagttt ggtagaagcc caagccccag aagatttaga
     5041 attgatccaa gctctccctt acatgaaaaa aaccttgaag ataacttaca gagtttggct
     5101 acacgattag ctccaattta taagcagtat gctccagtag cttaccaaaa tcaggtggaa
     5161 tatgaaaatg ttgcccgaga atgtcggctt ggcagcaagg aaggtcgacc cttctctggg
     5221 gtcactgctt gcctggactt ctgtgctcat ccccacaggg acattcacaa catgaataat
     5281 ggaagcactg tggtttgtac cttaactcga gaagataacc gctctttggg tgttattcct
     5341 caagatgagc agctccatgt gctacctctt tataagcttt cagacacaga tgagtttggc
     5401 tccaaggaag gaatggaagc caagatcaaa tctggggcca tcgaggtcct ggcaccccgc
     5461 cgcaaaaaaa gaacgtgttt cactcagcct gttccccgtt ctggaaagaa gagggctgcg
     5521 atgatgacag aggttcttgc acataagata agggcagtgg aaaagaaacc tattccccga
     5581 atcaagcgga agaataactc aacaacaaca aacaacagta agccttcgtc actgccaacc
     5641 ttagggagta acactgagac cgtgcaacct gaagtaaaaa gtgaaaccga accccatttt
     5701 atcttaaaaa gttcagacaa cactaaaact tattcgctga tgccatccgc tcctcaccca
     5761 gtgaaagagg catctccagg cttctcctgg tccccgaaga ctgcttcagc cacaccagct
     5821 ccactgaaga atgacgcaac agcctcatgc gggttttcag aaagaagcag cactccccac
     5881 tgtacgatgc cttcgggaag actcagtggt gccaatgctg cagctgctga tggccctggc
     5941 atttcacagc ttggcgaagt ggctcctctc cccaccctgt ctgctcctgt gatggagccc
     6001 ctcattaatt ctgagccttc cactggtgtg actgagccgc taacgcctca tcagccaaac
     6061 caccagccct ccttcctcac ctctcctcaa gaccttgcct cttctccaat ggaagaagat
     6121 gagcagcatt ctgaagcaga tgagcctcca tcagacgaac ccctatctga tgaccccctg
     6181 tcacctgctg aggagaaatt gccccacatt gatgagtatt ggtcagacag tgagcacatc
     6241 tttttggatg caaatattgg tggggtggcc atcgcacctg ctcacggctc ggttttgatt
     6301 gagtgtgccc ggcgagagct gcacgctacc actcctgttg agcaccccaa ccgtaatcat
     6361 ccaacccgcc tctcccttgt cttttaccag cacaaaaacc taaataagcc ccaacatggt
     6421 tttgaactaa acaagattaa gtttgaggct aaagaagcta agaataagaa aatgaaggcc
     6481 tcagagcaaa aagaccaggc agctaatgaa ggtccagaac agtcctctga agtaaatgaa
     6541 ttgaaccaaa ttccttctca taaagcatta acattaaccc atgacaatgt tgtcaccgtg
     6601 tccccttatg ctctcacaca cgttgcgggg ccctataacc attgggtctg aggccggcca
     6661 ggcaaaaaag aaaaagtaag aattcaaggg tgggcgcgcc gacccagctt tcttgtacaa
     6721 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaata
     6781 cccatacgac gtcccagact acgcttagta atgattaatt aaactagaaa ttctaccggg
     6841 taggggaggc gcttttccca aggcagtctg gagtaaccca cccaagatct ggcctccgcg
     6901 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagccgcg taagatctgg
     6961 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcctagtc
     7021 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     7081 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     7141 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     7201 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     7261 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     7321 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     7381 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     7441 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     7501 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     7561 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     7621 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     7681 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     7741 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     7801 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     7861 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     7921 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     7981 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     8041 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     8101 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     8161 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaagcggcc gccatgctag
     8221 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacggat ccataacttc
     8281 gtataatgta tgctatacga agttatgcca ccatgtctag actggacaag agcaaagtca
     8341 taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac
     8401 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     8461 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     8521 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     8581 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     8641 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     8701 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     8761 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     8821 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     8881 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     8941 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     9001 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
     9061 taagtaagga tccgcggccg cactagagga attccgcccc tctccccccc cccnnnnccc
     9121 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     9181 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     9241 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     9301 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     9361 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     9421 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     9481 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     9541 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     9601 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     9661 ctttgaaaaa cacgatgata agcttaccgg tccaccatgg tgagcaaggg cgaggagctg
     9721 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     9781 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     9841 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     9901 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     9961 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
    10021 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
    10081 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
    10141 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
    10201 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
    10261 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
    10321 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
    10381 gggatcactc tcggcatgga cgagctgtac aagtaagcat gcccgacggc gaggatctcg
    10441 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    10501 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    10561 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    10621 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    10681 gatgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
    10741 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    10801 gcaattgttg ttgtttagtc cctcccaatt cgatatcaag cttatcgcga tagatcctaa
    10861 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    10921 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    10981 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    11041 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    11101 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    11161 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    11221 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
    11281 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    11341 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    11401 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
    11461 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    11521 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    11581 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    11641 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    11701 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
    11761 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
    11821 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
    11881 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    11941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    12001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    12061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    12121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    12181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    12241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    12301 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    12361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    12421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    12481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    12541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    12601 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    12661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    12721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    12781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    12841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    12901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    12961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    13021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    13081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    13141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    13201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    13261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    13321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    13381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    13441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    13501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    13561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    13621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    13681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    13741 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    13801 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    13861 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    13921 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    13981 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    14041 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    14101 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    14161 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
    14221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    14281 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    14341 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    14401 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    14461 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
    14521 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
    14581 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
    14641 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
    14701 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    14761 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    14821 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    14881 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    14941 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    15001 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    15061 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    15121 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    15181 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    15241 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    15301 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    15361 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    15421 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    15481 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    15541 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    15601 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    15661 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    15721 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    15781 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    15841 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    15901 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    15961 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    16021 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    16081 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    16141 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    16201 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    16261 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    16321 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    16381 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    16441 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
    16501 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    16561 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    16621 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
    16681 actaggatcc tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
    16741 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
    16801 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
    16861 taccactccc tatcagtgat agagaaaagt gaaagtcgag
//
