LOCUS       sequence-194572-       10484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KIF20A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 10484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7130 pHAGE-P-CMVt-N-HA-GAW-KIF20A
FEATURES             Location/Qualifiers
     source          1..10484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     754..771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1011..1032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1047..1052,1053..1070,1071..1077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1085..1101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1090..1112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1109..1125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1109..1125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1175..1194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1386..2019
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2066..2191
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2688..2921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3106..3150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3443..3560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3612..3915
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3916..4119
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4070..4090
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4121..4139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4142..4160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4170..4194
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4281..4290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4293..4319
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4335..4359
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7030..7054)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7073..7572
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7108..7131)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7505..7524
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7593..8192
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7593..7612)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8089..8109
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8259..8847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8312..8332)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8730..8741)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8922..9155
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9274..9292)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9360..9464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9465..9533,9534..10325)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9683..9702)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
      841 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      901 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
      961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1081 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     1141 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1201 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     1261 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     1321 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     1381 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     1441 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     1501 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     1561 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     1621 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     1681 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     1741 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     1801 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     1861 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1921 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1981 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     2041 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     2101 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     2161 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     2221 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     2281 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     2341 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     2401 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     2461 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     2521 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     2581 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     2641 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2701 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2761 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2821 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2881 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2941 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3001 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3061 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3121 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3181 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3241 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     3301 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     3361 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac
     3421 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     3481 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     3541 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta
     3601 gtggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3901 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3961 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4021 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4081 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
     4141 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
     4201 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     4261 actctagcgt ttaaacgcta gccaccatgg cgtacccata cgatgttcca gattacgctg
     4321 ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcat gtcgcaaggg atcctttctc
     4381 cgccagcggg cttgctgtcc gatgacgatg tcgtagtttc tcccatgttt gagtccacag
     4441 ctgcagattt ggggtctgtg gtacgcaaga acctgctatc agactgctct gtcgtctcta
     4501 cctccctaga ggacaagcag caggttccat ctgaggacag tatggagaag gtgaaagtat
     4561 acttgagggt taggcccttg ttaccttcag agttggaacg acaggaagat cagggttgtg
     4621 tccgtattga gaatgtggag acccttgttc tacaagcacc caaggactct tttgccctga
     4681 agagcaatga acggggaatt ggccaagcca cacacaggtt caccttttcc cagatctttg
     4741 ggccagaagt gggacaggca tccttcttca acctaactgt gaaggagatg gtaaaggatg
     4801 tactcaaagg gcagaactgg ctcatctata catatggagt cactaactca gggaaaaccc
     4861 acacgattca aggtaccatc aaggatggag ggattctccc ccggtccctg gcgctgatct
     4921 tcaatagcct ccaaggccaa cttcatccaa cacctgatct gaagcccttg ctctccaatg
     4981 aggtaatctg gctagacagc aagcagatcc gacaggagga aatgaagaag ctgtccctgc
     5041 taaatggagg cctccaagag gaggagctgt ccacttcctt gaagaggagt gtctacatcg
     5101 aaagtcggat aggtaccagc accagcttcg acagtggcat tgctgggctc tcttctatca
     5161 gtcagtgtac cagcagtagc cagctggatg aaacaagtca tcgatgggca cagccagaca
     5221 ctgccccact acctgtcccg gcaaacattc gcttctccat ctggatctca ttctttgaga
     5281 tctacaacga actgctttat gacctattag aaccgcctag ccaacagcgc aagaggcaga
     5341 ctttgcggct atgcgaggat caaaatggca atccctatgt gaaagatctc aactggattc
     5401 atgtgcaaga tgctgaggag gcctggaagc tcctaaaagt gggtcgtaag aaccagagct
     5461 ttgccagcac ccacctcaac cagaactcca gccgcagtca cagcatcttc tcaatcagga
     5521 tcctacacct tcagggggaa ggagatatag tccccaagat cagcgagctg tcactctgtg
     5581 atctggctgg ctcagagcgc tgcaaagatc agaagagtgg tgaacggttg aaggaagcag
     5641 gaaacattaa cacctctcta cacaccctgg gccgctgtat tgctgccctt cgtcaaaacc
     5701 agcagaaccg gtcaaagcag aacctggttc ccttccgtga cagcaagttg actcgagtgt
     5761 tccaaggttt cttcacaggc cgaggccgtt cctgcatgat tgtcaatgtg aatccctgtg
     5821 catctaccta tgatgaaact cttcatgtgg ccaagttctc agccattgct agccagcttg
     5881 tgcatgcccc acctatgcaa ctgggattcc catccctgca ctcgttcatc aaggaacata
     5941 gtcttcaggt atcccccagc ttagagaaag gggctaaggc agacacaggc cttgatgatg
     6001 atattgaaaa tgaagctgac atctccatgt atggcaaaga ggagctccta caagttgtgg
     6061 aagccatgaa gacactgctt ttgaaggaac gacaggaaaa gctacagctg gagatgcatc
     6121 tccgagatga aatttgcaat gagatggtag aacagatgca acagcgggaa cagtggtgca
     6181 gtgaacattt ggacacccaa aaggaactat tggaggaaat gtatgaagaa aaactaaata
     6241 tcctcaagga gtcactgaca agtttttacc aagaagagat tcaggagcgg gatgaaaaga
     6301 ttgaagagct agaagctctc ttgcaggaag ccagacaaca gtcagtggcc catcagcaat
     6361 cagggtctga attggcccta cggcggtcac aaaggttggc agcttctgcc tccacccagc
     6421 agcttcagga ggttaaagct aaattacagc agtgcaaagc agagctaaac tctaccactg
     6481 aagagttgca taagtatcag aaaatgttag aaccaccacc ctcagccaag cccttcacca
     6541 ttgatgtgga caagaagtta gaagagggcc agaagaatat aaggctgttg cggacagagc
     6601 ttcagaaact tggtgagtct ctccaatcag cagagagagc ttgttgccac agcactgggg
     6661 caggaaaact tcgtcaagcc ttgaccactt gtgatgacat cttaatcaaa caggaccaga
     6721 ctctggctga actgcagaac aacatggtgc tagtgaaact ggaccttcgg aagaaggcag
     6781 catgtattgc tgagcagtat catactgtgt tgaaactcca aggccaggtt tctgccaaaa
     6841 agcgccttgg taccaaccag gaaaatcagc aaccaaacca acaaccacca gggaagaaac
     6901 cattccttcg aaatttactt ccccgaacac caacctgcca aagctcaaca gactgcagcc
     6961 cttatgcccg gatcctacgc tcacggcgtt cccctttact caaatctggg ccttttggca
     7021 aaaagtacta cccaactttc ttgtacaaag tggttcgata acgaattcta ccgggtaggg
     7081 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     7141 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     7201 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     7261 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     7321 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     7381 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     7441 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     7501 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     7561 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
     7621 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     7681 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     7741 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     7801 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     7861 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     7921 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     7981 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     8041 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     8101 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     8161 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     8221 ccgaaaggag cgcacgaccc catgcatcga tagatcctaa tcaacctctg gattacaaaa
     8281 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8341 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8401 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8461 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8521 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8581 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8641 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8701 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8761 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8821 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     8881 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8941 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9001 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9061 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9121 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9181 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     9241 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     9301 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9361 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9421 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9481 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9541 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9601 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9661 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     9721 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9781 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9841 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9901 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9961 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10021 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10081 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10141 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10201 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10261 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10321 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10381 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatc
//
