LOCUS       sequence-194577-       11072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PHKB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7138 pHAGE-P-CMVt-N-HA-GAW-PHKB
FEATURES             Location/Qualifiers
     source          1..11072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1013..1034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1049..1054,1055..1072,1073..1079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1087..1103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1092..1114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1111..1127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1111..1127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1177..1196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1388..2021
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2068..2193
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2690..2923
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3108..3152
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3445..3562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3614..3917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3918..4121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4072..4092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4123..4141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4144..4162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4172..4196
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4283..4292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4295..4321
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4337..4361
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7620..7644)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7663..8162
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7698..7721)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8095..8114
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8183..8782
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8183..8202)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8679..8699
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8849..9437
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8902..8922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9320..9331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9512..9745
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9864..9882)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9950..10054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10055..10123,10124..10915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10273..10292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
      841 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      961 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     1021 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     1081 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     1141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1201 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     1261 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     1321 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     1381 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     1441 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     1501 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     1561 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     1621 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     1681 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     1741 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     1801 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     1861 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1921 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1981 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2041 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2101 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2161 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2221 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2281 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     2341 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     2401 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     2461 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     2521 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     2581 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     2641 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     2701 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     2761 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     2821 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     2881 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     2941 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3001 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3061 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3121 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3181 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3241 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3301 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3361 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc
     3421 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     3481 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3541 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac
     3601 tagtggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3781 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3841 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3901 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4021 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4081 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag
     4141 atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct
     4201 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     4261 ggactctagc gtttaaacgc tagccaccat ggcgtaccca tacgatgttc cagattacgc
     4321 tgctcgaggt tatcaaacaa gtttgtacaa aaaagttggc atggcctgct cacctgatgc
     4381 agtcgtctct ccgtcttccg ctttcttaag gtctggctca gtttatgaac ctcttaaaag
     4441 cattaatctt ccaagacctg ataatgaaac tctctgggat aagttggacc attattacag
     4501 aattgtcaag tcaacattgc tgctgtatca aagtccaact accggtctct ttcccactaa
     4561 aacatgcggt ggtgaccaga aggccaagat ccaggacagc ctatactgcg ctgctggggc
     4621 ctgggctttg gctcttgcat acaggcgaat tgatgacgac aagggaagga cccatgagct
     4681 ggagcactca gctataaaat gcatgagagg aattctctac tgctatatgc gtcaggccga
     4741 taaggtccag cagtttaagc aggatccacg cccaacaaca tgtcttcact ctgttttcaa
     4801 tgtgcataca ggagatgagt tgctttccta tgaggaatat ggtcatcttc agataaatgc
     4861 agtgtcactt tatctccttt accttgtgga aatgatttcc tcaggactcc agattatcta
     4921 caacactgat gaggtctctt ttattcaaaa ccttgtattt tgtgtggaaa gagtttaccg
     4981 tgtgcctgac tttggtgtct gggaaagagg aagcaaatat aataatggca gcacagagct
     5041 acattcgagc tcggttggtt tagcaaaagc agctctagaa gcaattaatg gattcaacct
     5101 ttttggcaac cagggctgtt cgtggtcagt tatatttgtg gatctcgatg ctcacaatcg
     5161 caacaggcaa actttgtgct cgctgttacc cagagaatca agatcacata atacagatgc
     5221 tgccctgctc ccctgcatca gttatcctgc atttgccctg gatgatgaag ttctttttag
     5281 ccagacactt gataaagtgg ttagaaaatt aaaaggaaaa tatggattta aacgtttctt
     5341 gagagatggg tatagaacat cattggaaga tcccaacaga tgctactaca agccagctga
     5401 aattaagcta tttgatggca ttgaatgtga atttcccata tttttccttt atatgatgat
     5461 tgatggagtt tttagaggca atcctaagca agtacaggaa tatcaggatc ttttgactcc
     5521 agtacttcat cataccacag aaggatatcc tgttgtacca aagtactatt atgtgccagc
     5581 tgactttgta gaatatgaaa aaaataaccc tggtagtcaa aaacgatttc ctagcaactg
     5641 tggccgtgat ggaaaactgt ttctttgggg acaagcactt tatatcatcg caaaactcct
     5701 ggctgatgaa cttattagtc ctaaagacat tgatcctgtc cagcgctatg tcccactaaa
     5761 ggatcaacgt aacgtgagca tgaggttttc caatcagggc ccactggaaa atgacttggt
     5821 agttcatgtg gcacttatag cagaaagcca aagacttcaa gtttttctga acacatatgg
     5881 tattcaaact caaactcctc aacaagtaga acccattcag atatggcctc agcaggagct
     5941 tgtgaaagct tatttgcagc tgggtatcaa tgaaaagtta ggactctctg gaaggccaga
     6001 caggcccatt ggctgcctcg ggacatcaaa gatttatcgc attctaggaa agactgtggt
     6061 ttgttacccg attattttcg acctaagtga tttctacatg tctcaggatg ttttcctgct
     6121 gatagatgac ataaagaatg cgctgcagtt cattaaacaa tattggaaaa tgcatggacg
     6181 tccacttttc cttgttctca tccgggaaga caatataaga ggtagccggt tcaaccccat
     6241 attagatatg ctggcagccc ttaaaaaagg aataattgga ggagtcaaag ttcatgtgga
     6301 tcgtctacag acactaatat ctggagctgt ggtagaacaa cttgatttcc tacgaatcag
     6361 tgacacagaa gagcttccag aatttaagag ttttgaggaa ctagaacctc ccaaacattc
     6421 aaaagtcaaa cggcaaagca gcacccctag tgctcctgaa ctgggacagc agccggatgt
     6481 caacattagt gaatggaagg acaaacccac ccatgaaatt cttcaaaaac tgaatgattg
     6541 cagttgtctg gctagccaag ccatcctgct gggtatactg ctcaaaagag aaggccccaa
     6601 cttcatcaca aaggaaggta ccgtttctga tcacattgag agagtctata gaagagctgg
     6661 cagccaaaaa ctttggtcgg ttgtacgccg tgcagcaagt cttttaagta aagtagtgga
     6721 cagcctggcc ccatccatta ctaatgtttt agtgcagggc aaacaggtaa ctctgggtgc
     6781 ctttgggcat gaagaagaag ttatctctaa tcctttgtct ccaagagtga ttcaaaacat
     6841 catctattat aagtgtaaca cccatgatga gagggaagcg gtcattcagc aagaactggt
     6901 catccatatt ggctggatca tctccaataa ccctgagtta ttcagtggca tgctgaaaat
     6961 acgaatcggg tggatcatcc atgccatgga gtatgaactt cagatccgtg gcggagacaa
     7021 gccagccttg gacttgtatc agctgtcacc tagtgaagtt aaacagcttc tgctggatat
     7081 tctgcagcct caacagaatg gaagatgttg gctgaacagg cgtcagatcg atgggtcttt
     7141 gaatagaact cccaccgggt tctatgaccg agtgtggcag attctggagc gcacgcccaa
     7201 tgggatcatt gttgctggga agcatttgcc tcagcaacca accctgtcag atatgaccat
     7261 gtatgagatg aatttctctc tccttgttga agacacgttg ggaaatattg accagccaca
     7321 gtacagacag atcgttgtag agttacttat ggttgtatcc attgtactgg aaagaaaccc
     7381 cgagctagaa tttcaagaca aagtagatct agacagactg gtcaaagaag catttaatga
     7441 atttcaaaaa gatcagagtc ggctaaagga aattgaaaaa caagatgaca tgacttcctt
     7501 ttacaacact cctcccctgg gaaaaagagg aacatgcagc tatttgacaa aggcggtgat
     7561 gaatctgctg ctggaaggag aagtcaagcc aaacaatgat gacccgtgtc tgattagcta
     7621 cccaactttc ttgtacaaag tggttcgata acgaattcta ccgggtaggg gaggcgcttt
     7681 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     7741 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     7801 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     7861 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     7921 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     7981 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     8041 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     8101 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     8161 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     8221 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     8281 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     8341 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     8401 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     8461 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     8521 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     8581 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     8641 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     8701 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8761 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     8821 cgcacgaccc catgcatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
     8881 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8941 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     9001 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     9061 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     9121 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     9181 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     9241 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     9301 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     9361 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     9421 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     9481 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     9541 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     9601 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9661 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9721 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     9781 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     9841 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     9901 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9961 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    10021 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10081 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10141 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10201 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10261 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    10321 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10381 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10441 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10501 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10561 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10621 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10681 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10741 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10801 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10861 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10921 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10981 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11041 gttccactga gcgtcagacc ccgtagaaaa ga
//
