LOCUS       sequence-194584-        9068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PRKAR2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B
FEATURES             Location/Qualifiers
     source          1..9068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     517..536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     770..787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1027..1048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1063..1068,1069..1086,1087..1093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1101..1117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1106..1128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1125..1141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1125..1141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1191..1210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1402..2035
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2082..2207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2704..2937
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3122..3166
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3459..3576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3628..3931
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3932..4135
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4086..4106
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4137..4155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4158..4176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4186..4210
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4297..4306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4309..4335
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4351..4375
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5630..5654)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5673..6172
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5708..5731)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6105..6124
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6193..6792
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6193..6212)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6689..6709
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6859..7447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6912..6932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7330..7341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7522..7755
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7874..7892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7960..8064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8065..8133,8134..8925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8283..8302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
       61 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      781 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      841 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
      901 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      961 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1021 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1081 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     1141 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1201 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     1261 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     1321 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     1381 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     1441 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     1501 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     1561 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     1621 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     1681 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     1741 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     1801 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     1861 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     2041 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     2101 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     2161 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     2221 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     2281 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     2341 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     2401 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     2461 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     2521 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     2581 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     2641 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     2701 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     2761 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     2821 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     2881 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     2941 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     3001 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     3061 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     3121 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     3181 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     3241 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     3301 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     3361 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     3421 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     3481 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     3541 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     3601 gatccagttt ggactagtgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     3661 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3721 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     3781 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     3841 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     3901 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     3961 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     4021 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     4081 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
     4141 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
     4201 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     4261 ccgatccagc ctccggactc tagcgtttaa acgctagcca ccatggcgta cccatacgat
     4321 gttccagatt acgctgctcg aggttatcaa acaagtttgt acaaaaaagt tggcatgagc
     4381 atcgagatcc cggcgggact gacggagctg ctgcagggct tcacggtgga ggtgctgagg
     4441 caccagcccg cggacctgct ggagttcgct ctgcagcact tcacccgcct gcagcaggag
     4501 aacgagcgca aaggcaccgc gcgcttctgc catgagggca ggacctgggg ggacctgggc
     4561 gccgctgccg ggggcggcac ccccagcaag ggggtcaact tcgccgagga gcccatgcag
     4621 tccgactccg aggacgggga ggaggaggag gcggcgcccg cggacgcagg ggcgttcaat
     4681 gctccagtaa taaaccgatt cacaaggcgt gcctcagtat gtgcagaagc ttataatcct
     4741 gatgaagaag aagatgatgc agagtccagg attatacatc caaaaactga tgatcaaaga
     4801 aataggttgc aagaggcttg caaagacatc ctgctgttta agaatctgga tccggagcag
     4861 atgtctcaag tattagatgc catgtttgaa aaattggtca aagatgggga gcatgtaatt
     4921 gatcaaggtg acgatggtga caacttttat gtaattgata gaggcacatt tgatatttat
     4981 gtgaaatgtg atggtgttgg aagatgtgtt ggtaactatg ataatcgtgg gagtttcggc
     5041 gaactggcct taatgtacaa tacacccaga gcagctacaa tcactgctac ctctcctggt
     5101 gctctgtggg gtttggacag ggtaaccttc aggagaataa ttgtgaaaaa caatgccaaa
     5161 aagagaaaaa tgtatgaaag ctttattgag tcactgccat tccttaaatc tttggagttt
     5221 tctgaacgcc tgaaagtagt agatgtgata ggcaccaaag tatacaacga tggagaacaa
     5281 atcattgctc agggagattc ggctgattct tttttcattg tagaatctgg agaagtgaaa
     5341 attactatga aaagaaaggg taaatcagaa gtggaagaga atggtgcagt agaaatcgct
     5401 cgatgctcgc ggggacagta ctttggagag cttgccctgg taactaacaa acctcgagca
     5461 gcttctgccc acgccattgg gactgtcaaa tgtttagcaa tggatgtgca agcatttgaa
     5521 aggcttctgg gaccttgcat ggaaattatg aaaaggaaca tcgctaccta tgaagaacag
     5581 ttagttgccc tgtttggaac gaacatggat attgttgaac ccactgcatg cccaactttc
     5641 ttgtacaaag tggttcgata acgaattcta ccgggtaggg gaggcgcttt tcccaaggca
     5701 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     5761 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     5821 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     5881 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     5941 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     6001 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     6061 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     6121 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     6181 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6241 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6301 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6361 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6421 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6481 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6541 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6601 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6661 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6721 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6781 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     6841 catgcatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6901 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6961 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7021 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7081 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7141 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7201 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7261 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7321 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7381 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7441 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7501 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     7561 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7621 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7681 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7741 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7801 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
     7861 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     7921 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8281 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     8341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8581 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9001 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9061 gcgtcaga
//
