LOCUS       sequence-194606-       12089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ZFYV9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 12089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9
FEATURES             Location/Qualifiers
     source          1..12089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     526..545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     779..796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1036..1057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1072..1077,1078..1095,1096..1102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1110..1126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1115..1137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1134..1150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1134..1150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1200..1219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1411..2044
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2091..2216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2713..2946
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3131..3175
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3468..3585
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3637..3940
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3941..4144
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4095..4115
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4146..4164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4167..4185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4195..4219
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4306..4315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4318..4344
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4360..4384
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8660..8684)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8703..9202
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8738..8761)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9135..9154
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9223..9822
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9223..9242)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9719..9739
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9889..10477
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9942..9962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10360..10371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10552..10785
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10904..10922)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10990..11094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11095..11163,11164..11955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11313..11332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
       61 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      841 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
      901 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      961 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
     1021 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     1081 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1141 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
     1201 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     1261 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     1321 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     1381 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     1441 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     1501 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     1561 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     1621 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     1681 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     1741 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     1801 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     1861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     1921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2041 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     2101 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     2161 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     2221 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     2281 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     2341 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     2401 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     2461 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     2521 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     2581 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     2641 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     2701 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     2761 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     2821 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2881 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2941 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3001 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3061 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3121 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3181 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3241 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     3301 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     3361 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     3421 atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
     3481 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     3541 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     3601 gacagcagag atccagtttg gactagtgga gttccgcgtt acataactta cggtaaatgg
     3661 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3721 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3781 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3841 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3901 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     3961 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4021 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     4081 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     4141 agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg
     4201 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     4261 acaccgggac cgatccagcc tccggactct agcgtttaaa cgctagccac catggcgtac
     4321 ccatacgatg ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagtt
     4381 ggcatggaga attacttcca agcagaagct tacaacctgg acaaggtgtt agatgaattt
     4441 gaacaaaacg aagatgaaac agtttcttct actttattgg atacaaagtg gaataagatt
     4501 ctagatcccc cttctcaccg gctgtcattt aaccctactt tggccagtgt gaatgaatct
     4561 gcagtttcta atgagtcaca accacaactg aaagtcttct ccctggctca ttcagctccc
     4621 ctgaccacag aggaagagga tcactgtgct aatggacagg actgtaatct aaatccagag
     4681 attgccacaa tgtggattga tgaaaatgct gttgcagaag accagttaat taagagaaac
     4741 tatagttggg atgatcaatg cagtgctgtt gaagtgggag agaagaaatg tggaaacctg
     4801 gcttgtctgc cagatgagaa gaatgttctt gttgtagccg tcatgcataa ctgtgataaa
     4861 aggacattac aaaacgattt acaggattgt aataattata atagtcaatc ccttatggat
     4921 gcttttagct gttcactgga taatgaaaac agacaaactg atcaatttag ttttagtata
     4981 aatgagtcca ctgaaaaaga tatgaattca gagaaacaaa tggatccatt gaatagaccg
     5041 aaaacagagg ggagatctgt taaccatctg tgtcctactt catctgatag tctagccagt
     5101 gtctgttccc cttcacaatt aaaggatgac ggaagtatag gtagagaccc ctccatgtct
     5161 gcgattacaa gtttaacggt tgattcagta atctcatccc agggaacaga tggatgtcct
     5221 gctgttaaaa agcaagagaa ctatatacca gatgaggacc tcactggcaa aatcagctct
     5281 cctaggacag atctagggag tccaaattcc ttttcccaca tgagtgaggg gattttgatg
     5341 aaaaaagagc cagcagagga gagcaccact gaagaatccc tccggtctgg tttacctttg
     5401 cttctcaaac cagacatgcc taatgggtct ggaaggaata atgactgtga acggtgttca
     5461 gattgccttg tgcctaatga agttagggct gatgaaaatg aaggttatga acatgaagaa
     5521 actcttggca ctacagaatt ccttaatatg acagagcatt tctctgaatc tcaggacatg
     5581 actaattgga agttgactaa actaaatgag atgaatgata gccaagtaaa cgaagaaaag
     5641 gaaaagtttc tacagattag tcagcctgag gacactaatg gtgatagtgg aggacagtgt
     5701 gttggattgg cagatgcagg tctagattta aaaggaactt gcattagtga aagtgaagaa
     5761 tgtgatttct ccactgttat agacacacca gcagcaaatt atctatctaa tggttgtgat
     5821 tcctatggaa tgcaagaccc aggtgtttct tttgttccaa agactttacc ctccaaagaa
     5881 gattcagtaa cagaagaaaa agaaatagag gaaagcaagt cagaatgcta ctcaaatatt
     5941 tatgaacaga gaggaaatga ggccacagaa gggagtggac tacttttaaa cagcactggt
     6001 gacctaatga agaaaaatta tttacataat ttctgtagtc aagttccatc agtgcttggg
     6061 caatcttccc ccaaggtagt agcaagcctg ccatctatca gtgttccttt tggtggtgca
     6121 agacccaagc aaccttctaa tcttaaactt caaattccaa agccattatc agaccattta
     6181 caaaatgact ttcctgcaaa cagtggaaat aatactaaaa ataaaaatga tattcttggg
     6241 aaagcaaaat taggggaaaa ctcagcaacc aatgtatgca gtccatcttt gggaaacatc
     6301 tctaatgtcg atacaaatgg ggaacattta gaaagttatg aggctgagat ctccactaga
     6361 ccatgccttg cattagctcc agatagccca gataatgatc tcagagctgg tcagtttgga
     6421 atttctgcca gaaagccatt caccactctg ggtgaggtgg ctccagtatg ggtaccggat
     6481 tctcaggctc caaattgcat gaaatgtgaa gccaggttta cattcaccaa aaggaggcat
     6541 cactgcagag catgtgggaa ggttttctgt gcttcctgct gtagcctgaa atgtaaactg
     6601 ttatacatgg acagaaagga agctagagtg tgtgtaatct gccattcagt gctaatgaat
     6661 gctcaagcct gggagaacat gatgagtgcc tcaagccaga gccctaaccc taacaatcct
     6721 gctgaatact gttctactat ccctcccttg cagcaagctc aggcctcagg agctctgagc
     6781 tctccacctc ccactgtgat ggtacctgtg ggagttttaa agcaccctgg agcagaagtg
     6841 gctcagccca gagagcagag gcgagtttgg tttgctgatg ggatcttgcc caatggagaa
     6901 gttgctgatg cagccaaatt aacaatgaat ggaacttcct ctgcaggaac cctggctgtg
     6961 tcacacgacc cagtcaagcc agtaactacc agtcctctac cagcagagac ggatatttgt
     7021 ctattctctg ggagtataac tcaggttgga agtcctgttg gaagtgcaat gaatcttatt
     7081 cctgaagatg gccttcctcc cattctcatc tccactggtg taaaaggaga ctatgctgtg
     7141 gaagagaaac catcacagat ttcagtaatg cagcagttgg aggatggtgg ccctgaccca
     7201 cttgtatttg ttttaaatgc aaatttgttg tcaatggtta aaattgtaaa ttatgtgaac
     7261 aggaagtgct ggtgtttcac aaccaaggga atgcatgcag tgggtcagtc tgagatagtc
     7321 attcttctac agtgtttacc ggatgaaaag tgtttgccaa aggatatctt taatcacttt
     7381 gtgcagcttt atcgggatgc tctggcaggg aatgtggtga gcaacttggg acattccttc
     7441 ttcagtcaaa gtttccttgg cagtaaagaa catggtggat tcttatatgt gacatctacc
     7501 taccagtcac tgcaagacct agtactccca accccacctt acttgtttgg gattcttatc
     7561 cagaaatggg aaactccttg ggctaaagta tttcctatcc gtctgatgtt gagacttgga
     7621 gctgaatatc gactttatcc atgcccacta ttcagtgtca gatttcggaa gccattgttt
     7681 ggagagacgg ggcataccat catgaatctt cttgcagact tcagaaatta ccagtatacc
     7741 ttgccagtag ttcaaggttt ggtggttgat atggaagttc ggaaaactag catcaaaatt
     7801 cccagcaaca gatacaatga gatgatgaaa gccatgaaca agtccaatga gcatgtcctg
     7861 gcaggaggtg cctgcttcaa tgaaaaggca gactctcatc ttgtgtgtgt acagaatgat
     7921 gatggaaact atcagaccca ggctatcagt attcacaatc agcccagaaa agtgactggt
     7981 gccagtttct ttgtgttcag tggcgctctg aaatcctctt ctggatacct tgccaagtcc
     8041 agtattgtgg aagatggtgt tatggtccag attactgcag agaacatgga ttccttgagg
     8101 caggcactgc gagagatgaa ggacttcacc atcacctgtg ggaaggcgga cgcggaggaa
     8161 ccccaggagc acatccacat ccagtgggtg gatgatgaca agaacgttag caagggtgtc
     8221 gtaagtccta tagatgggaa gtccatggag actataacaa atgtgaagat attccatgga
     8281 tcagaatata aagcaaatgg aaaagtaatc agatggacag aggtgttttt cctagaaaac
     8341 gatgaccagc acaattgcct cagtgatcct gcagatcaca gtagattgac tgagcatgtt
     8401 gccaaagctt tttgccttgc tctctgtcct cacctgaaac ttctgaagga agatggaatg
     8461 accaaactgg gactacgtgt gacacttgac tcagatcagg ttggctatca agcagggagc
     8521 aatggccagc cccttccctc gcagtacatg aatgatctgg acagcgcctt ggtgccggtg
     8581 atccatggag gggcctgcca gcttagtgag ggccccgttg tcatggaact catcttttat
     8641 attctggaaa acatcgtata cccaactttc ttgtacaaag tggttcgata acgaattcta
     8701 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     8761 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     8821 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     8881 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     8941 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     9001 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     9061 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     9121 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     9181 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     9241 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     9301 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     9361 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     9421 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     9481 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     9541 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     9601 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     9661 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     9721 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     9781 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     9841 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tagatcctaa tcaacctctg
     9901 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     9961 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10021 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10081 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10141 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10201 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10261 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    10321 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10381 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10441 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
    10501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    10561 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    10621 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10681 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10741 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
    10801 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
    10861 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
    10921 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    10981 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    11041 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    11101 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    11161 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    11221 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    11281 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
    11341 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    11401 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    11461 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    11521 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    11581 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    11641 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    11701 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    11761 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11821 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11881 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11941 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    12001 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    12061 atcccttaac gtgagttttc gttccactg
//
