LOCUS       sequence-194625-        6774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV vector that expresses nuclear localized iRFP under control of
            the c-fos promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC588 - pAAV c-fos Nuc-iRFP
FEATURES             Location/Qualifiers
     source          1..6774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   123..252
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             complement(966..977)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1943..2890
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             2924..2944
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2948..2968
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2972..2992
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3023..3611
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3076..3096)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3494..3505)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3643..4119
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3842..3861)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4159..4299
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4159..4288
                     /label=AAV2 ITR
     rep_origin      4374..4829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4461..4480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4671..4692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4846..4865)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4965..4987
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5025..5043)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5111..5215
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5216..5284,5285..6076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5434..5453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6247..6774,1..61)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6736..6755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
       61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      121 gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct
      181 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
      241 ctaggggttc ctgcggccgc acgcgtaagc tttcctttag gaacagaggc ttcgagcctt
      301 taaggctgcg tacttgcttc tcctaatacc agagactcaa aaaaaaaaaa aagttccaga
      361 ttgctggaca atgacccggg tctcatccct tgaccctggg aaccgggtcc acattgaatc
      421 aggtgcgaat gttcgctcgc cttctctgcc tttcccgcct cccctccccc ggccgcggcc
      481 ccggttcccc ccctgcgctg caccctcaga gttggctgca gccggcgagc tgttcccgtc
      541 aatccctccc tcctttacac aggatgtcca tattaggaca tctgcgtcag caggtttcca
      601 cggccggtcc ctgttgttct ggggggggga ccatctccga aatcctacac gcggaaggtc
      661 taggagaccc cctaagatcc caaatgtgaa cactcatagg tgaaagatgt atgccaagac
      721 gggggttgaa agcctggggc gtagagttga cgacagagcg cccgcagagg gccttggggc
      781 gcgcttcccc ccccttccag ttccgcccag tgacgtagga agtccatcca ttcacagcgc
      841 ttctataaag gcgccagctg aggcgcctac tactccaacc gcgactgcag cgagcaactg
      901 agaagactgg atagagccgg cggttccgcg aacgagcagt gaccgcgctc ccacccagct
      961 ctgctctgca gctcccacca gtgtctaccc ctggacccct tgccgggctt tccccaaact
     1021 tcgaccatga tgttctcggg tttcaacgcc gactacgagg cgtcatcctc ccgctgcagt
     1081 agcgcctccc cggccgggga cagcctttcc tactaccatt ccccagccga ctccttctcc
     1141 agcatgggct ctcctgtcaa cacacaggtg agtttggctt tgtgtagccg ccaggtccgc
     1201 gctgagggtc gccgtggagg agacactggg gtgtgactcg caggggcggg ggggtcttcc
     1261 tttttcgctc tggagggaga ctggcgcggt cagagcagcc ttagcctggg aacccaggac
     1321 ttgtctgagc gcgtgcacac ttgtcatagt aagacttagt gaccccttcc cgcgcggcag
     1381 gtttattctg agtggcctgc ctgcattctt ctctcggccg acttgtttct gagatcagcc
     1441 ggggccaaca agtctcgagc aaagagtcgc taactagagt ttgggaggcg gcaaaccgcg
     1501 gcaatccccc ctcccggggc agcctggagc agggaggagg gaggagggag gagggtgctg
     1561 cgggcgggtg tgtaaggcag tttcattgat aaaaagcgag ttcattctgg agactccgga
     1621 gcagcgcctg cgtcagcgca gacgtcaggg atatttataa caaaccccct ttcgagcgag
     1681 tgatgccgaa gggataacgg gaacgcagca gtaggatgga ggggaaaggc tgcgctgcgg
     1741 aattcaaggg aggatattgg gagagctttt atctccgatg aggtgcatac aggaagacat
     1801 aagcagtctc tgaccggaat gcttctctct ccctgcttca tgcgacacta gggccacttg
     1861 ctccacctgt gtctggaacc tcctcgctca cctccgcttt cctctttttg ttttgtttca
     1921 ggacttttgc gcagatctct ccatggctga aggatccgtc gccaggcagc ctgacctctt
     1981 gacctgcgac gatgagccga tccatatccc cggtgccatc caaccgcatg gactgctgct
     2041 cgccctcgcc gccgacatga cgatcgttgc cggcagcgac aaccttcccg aactcaccgg
     2101 actggcgatc ggcgccctga tcggccgctc tgcggccgat gtcttcgact cggagacgca
     2161 caaccgtctg acgatcgcct tggccgagcc cggggcggcc gtcggagcac cgatcactgt
     2221 cggcttcacg atgcgaaagg acgcaggctt catcggctcc tggcatcgcc atgatcagct
     2281 catcttcctc gagctcgagc ctccccagcg ggacgtcgcc gagccgcagg cgttcttccg
     2341 ccgcaccaac agcgccatcc gccgcctgca ggccgccgaa accttggaaa gcgcctgcgc
     2401 cgccgcggcg caagaggtgc ggaagattac cggcttcgat cgggtgatga tctatcgctt
     2461 cgcctccgac ttcagcggcg aagtgatcgc agaggatcgg tgcgccgagg tcgagtcaaa
     2521 actaggcctg cactatcctg cctcaaccgt gccggcgcag gcccgtcggc tctataccat
     2581 caacccggta cggatcattc ccgatatcaa ttatcggccg gtgccggtca ccccagacct
     2641 caatccggtc accgggcggc cgattgatct tagcttcgcc atcctgcgca gcgtctcgcc
     2701 cgtccatctg gaattcatgc gcaacatagg catgcacggc acgatgtcga tctcgatttt
     2761 gcgcggcgag cgactgtggg gattgatcgt ttgccatcac cgaacgccgt actacgtcga
     2821 tctcgatggc cgccaagcct gcgagctagt cgcccaggtt ctggcctggc agatcggcgt
     2881 gatggaagag ttgtacaagt ccggcctgcg cagccgcgcc gacccaaaaa agaagcgcaa
     2941 ggtagacccc aagaagaagc gcaaggtaga tccaaaaaag aagagaaagg tatgatagga
     3001 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     3061 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3121 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3181 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3241 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3301 aactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3361 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3421 atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt
     3481 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3541 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3601 cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac
     3661 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     3721 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     3781 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     3841 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     3901 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     3961 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     4021 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     4081 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg
     4141 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4201 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4261 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     4321 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     4381 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4441 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4501 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4561 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     4621 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4681 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     4741 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4801 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     4861 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4921 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4981 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5041 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5101 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5161 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5221 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5281 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5341 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5401 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5461 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5521 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5581 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5641 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5701 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5761 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5821 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5881 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5941 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6001 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6061 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6121 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6181 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6241 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6301 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6361 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6421 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6481 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6541 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6601 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6661 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6721 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcg
//
