LOCUS       sequence-194628-        7337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid can be either plasmid propogation backbone or expression
            vector in specific strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7337)
  TITLE     New shuttle vector for Synechocystis sp. PCC6803
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSCB-YFP
FEATURES             Location/Qualifiers
     source          1..7337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(168..173,174..190,191..197)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    205..221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             239..250
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(257..259,260..262,263..976)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(302..323)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(563..582)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     910..931
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      996..1067
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1083..1110
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             4915..5106
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5100..5122)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5208..5348
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5263..5282
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5363..5380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5534..6122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5614..5633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6402..7193)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
       61 gttgcgtgac gcggccgcca gatcttccgg atggctcgag tttttcagca agatatctta
      121 ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatggttg acaattaatc
      181 atccggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ttctagagaa
      241 agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      301 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      361 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      421 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta
      481 ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      541 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      601 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      661 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
      721 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      781 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      841 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
      901 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      961 cgagctgtac aagtaataat actagtgcca ggcatcaaat aaaacgaaag gctcagtcga
     1021 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1081 ctggctcacc ttcgggtggg cctttctgcg tttatataaa cagcgtgaca gcgaccgatc
     1141 taatccgtta caattctggc aatagtccaa gatttccaac aaaaaaaagc ttttcaggag
     1201 ggaattaaga ttgctgcagt aaaaaacgta agaagtttag ttgacgctaa aaaacttacc
     1261 tacagacaat aacccggccc aaaaagccaa caaaatactt caaaaatatt gtctctactg
     1321 tagctctaaa aattcccaaa agaaaagcgg tcaactcttg aacccgagac cgctatgaac
     1381 aattatagtc tttcccacca aaattctcaa gccttagccc atcagcattt caacggatgg
     1441 caagggtcaa aaaatgaccc aaaaatacgg tcagaatttg tagaagattc ggcgatcgcc
     1501 cctgacgtat tccgggccaa cgtggagatt gtgccggatc ttgagataga ccccatcacc
     1561 aaggaagtct tggcaacgcc gatcgccgat gccctggggc gaaaatttac ccgctttgga
     1621 catcaagcca aggaagacag gcaggccgcc attttccggt ctgagaatgg gaacgtatgg
     1681 caggtaaaaa tctttggtga agacaagacg ggcaagcggt cagggcagta tctagcaccg
     1741 acaggcatag gggacgtgcc ctatctgcca acaattcccc gccggatcat tttggcgatc
     1801 gccgaaaagc atggggtcaa accaccggaa gatggtcaag atttttggcc ctggtttgtg
     1861 gatcatcctg agattccctt gattgtgacg gaagggggga agaaagcttt agccgccatc
     1921 agccaaggtt acgtggccct gagtctctat ggctgtttat gtggcaacga tggtctaacc
     1981 atcaaaccgt cattattgcc ctatgtgcag gggcgagaag tagcgatcgc ctatgaccag
     2041 gatgcaaagg gtagcaaggg acgaaaggca gtctttaagg gcacaaaacg gctggctcgt
     2101 aacctgacct atcacgctaa ggcaacggtc aagatctgcc aatggaacgg ggaagacggc
     2161 aagggcattg atgacttgat cgccaataac cctgctaaat ttcaccgggc gatcgctcgg
     2221 ggcatcagcc tagaacagtt ccaaaacaag ccgtatgctg acctatccca atgggtgaat
     2281 caaacccggc acgaaagata cctaagcaac attaatgccc ctaccaccgc caaaataatc
     2341 gggattaaat cagccaaggg cacgggaaag acggaagcga tctcaaaaat ggtggagcaa
     2401 cggcgagaag attttcgccg gacttatcca ctcactcacc gaattcaact aggcattgcc
     2461 ctatccggcc ggtttggtgt ggactacatc agagatttgt ccgagagcga cactaatggg
     2521 gccttaggaa tcggattgtg cattgatagc atcctgaaac ttgaccccga aacattccgg
     2581 ggccatgaca tcatccttga tgaatgcgag caattgatat gggaattatt agatagttcc
     2641 cggggaaacc tgggcaaaca taaaccagcc atcttggccc gcttccaaga aatattgatc
     2701 gccgccgctg attcgggggg caagattatt ttgtctgatg ctgatttgtc cccggtgact
     2761 atcaagtatg tccaagactt aatcggtggt aaggcagaat gctatattgt cgttaacacg
     2821 tttaagccag tgcaggataa gcgtaatttg attgtttata attccccgga agatttgctt
     2881 acttccgcta ttaatactgt tgctaatggt aaaaaaatac agatttgcac cggggcacaa
     2941 aaagcacagt ctatctattc aacaactaac ctaggggact tattcaaaat gttattccct
     3001 gataagtcag taggggtctt agatgcccat acagtaagcg ataagactaa ccctgcctat
     3061 ggctgtatgg ataacttaga tagatattta gaagacaagg atattgtcat cacgtccccg
     3121 gtaatagaga cgggaatcag cattgataag ccctattttg atgaagtcta tggattcgga
     3181 cagggaacac aaacagtaga gcaattctgc caaggattag aaaggtatcg tcccgatgtg
     3241 gatcgccatg tttggattcg caatcgtgcc cctaataatt gttttattgc tggcggggaa
     3301 actaaccctc gctatatcct taatgtggag aataagaaag ccaaggctac tatcggggca
     3361 ttgaatacag ccgacaattt aaccctgttt gatgatgaga agccagagca tcttaaaacc
     3421 tgggctatta tggcggctct gcacaatcaa gggaagaaaa gcttaaaatc aactactctt
     3481 gaaaaattag gggaagaagg gtacacacag aaagaacctg acccagatat ggtcaccggg
     3541 gcggaagacg tagctgatcg cattaaagct atccgtgacc ataactatgg aaaggaaacc
     3601 acggcgatcg ccgagagcct taatcccgat gacttcacct atgaaagttt gaagcggaag
     3661 caagttaaga cggaagcgga acgatatcaa gagcggaagg gagatctttg ccgtgccctg
     3721 gctaccgatg acataacccc agaagatgtg gagaaatgtg atcaaggatg gctggcccaa
     3781 ttgaccctgc attattactt caccaccggt gcagaatttt tacaggcaag ggacagccaa
     3841 aggctccaaa atttggccgg ggaagatgga aaggtttgtg ccaaggatgc tagccggaag
     3901 actctcaccg tccccgttca cttccttaaa gtattgggct ttgaaagatt ccttaccggg
     3961 ataggggaat tttccaagga ttccctagcc aattggtttg agaagactat cctgcccaac
     4021 aaggcagaaa ttaagcaggc cttgggcatc acgatcaacc ctgacaaagg ggcgatcgcc
     4081 ttcgtgcaat ctgtcttgaa aaaaatgggt ttacatcttg agtatctggg ccggtttggg
     4141 gaacggggca atactaagcg acattatggc cccgccaata ttgaccccga tgggcgatcg
     4201 gctatttttt cccggtggtt tgagcgggat tctattttgt atcactctga taccgtatcc
     4261 actgaatcct tattaattaa tcaagcctag gaactggata ccaaaaacag ggagttatga
     4321 tgggaatata atcccgttaa caggctaaac ccatgcaggc aaaccaaccc ttggcagaac
     4381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     4441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     4501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     4561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     4621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     4681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     4741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     4801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     4861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     4921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     4981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     5041 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     5101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     5221 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     5281 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     5341 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5941 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agacttgacc
     6301 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     6361 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     6421 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     6481 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     6541 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     6601 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     6661 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     6721 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     6781 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     6841 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     6901 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     6961 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     7021 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     7081 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     7141 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     7201 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     7261 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     7321 aaaaaacagt cataaca
//
