LOCUS       sequence-194629-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9, REC2 FRET variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC033 - Bacterial expression plasmid for SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     395..412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    427..566
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(492..511)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     652..674
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(668..859)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2317..2336
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2420..2439
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2420..2438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2527..2549
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2569..2598
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2608..3708
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     3682..3705
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3763..3783
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3790..7896
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETACATRLKRTARRRYTRRKNRISYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDCDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIESFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(8022..8040)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8036..8083
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8449..8454,8455..8471,8472..8477))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8504..8522)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8590..8694
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8695..8763,8764..9555)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8913..8932)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9726..10073,1..241)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
       61 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      361 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      421 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      481 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      541 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      601 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      661 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      721 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      781 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      841 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
      901 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
      961 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     1021 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     1081 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     1141 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     1201 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     1261 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     1321 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
     1381 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     1441 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     1501 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     1561 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     1621 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     1681 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     1741 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     1801 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     1861 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     1921 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     1981 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     2041 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     2101 ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     2161 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     2221 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     2281 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     2341 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     2401 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
     2461 agtgccggct ccggagagct ctttaattaa gcggccgccc tgcaggactc gagttctaga
     2521 aataattttg tttaacttta agaaggagat atacatatga aatcttctca ccatcaccat
     2581 caccatcacc atcaccatgg ttcttctatg aaaatcgaag aaggtaaact ggtaatctgg
     2641 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     2701 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     2761 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     2821 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     2881 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     2941 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     3001 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     3061 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     3121 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcaaaagcg
     3181 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     3241 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     3301 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     3361 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     3421 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     3481 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     3541 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     3601 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     3661 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     3721 aacaacaaca acaataacaa taacaacaac ctcgggatcg aggaaaacct gtacttccaa
     3781 tccaatgcaa tggataagaa atactcaata ggcttagata tcggcacaaa tagcgtcgga
     3841 tgggcggtga tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat
     3901 acagaccgcc acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag
     3961 acagcgtgtg cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat
     4021 cgtatttctt atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc
     4081 tttcatcgac ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct
     4141 atttttggaa atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat
     4201 ctgcgaaaaa aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc
     4261 ttagcgcata tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat
     4321 aatagtgatg tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa
     4381 gaaaacccta ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt
     4441 aaatcaagac gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta
     4501 tttgggaatc tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat
     4561 ttggcagaag atgctaaatt acagctttca aaagatactt acgattgtga tttagataat
     4621 ttattggcgc aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca
     4681 gatgctattt tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta
     4741 tcagcttcaa tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct
     4801 ttagttcgac aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac
     4861 ggatatgcag gttatattga tgggggagct agccaagaag aattttataa atttatcaaa
     4921 ccaattttag aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat
     4981 ttgctgcgca agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt
     5041 gagctgcatg ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt
     5101 gagaagattg aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt
     5161 ggcaatagtc gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat
     5221 tttgaagaag ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac
     5281 tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat
     5341 tttacggttt ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca
     5401 gcatttcttt caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga
     5461 aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa aaatagaatc ttttgatagt
     5521 gttgaaattt caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg
     5581 ctaaaaatta ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag
     5641 gatattgttt taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa
     5701 acatatgctc acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact
     5761 ggttggggac gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa
     5821 acaatattag attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc
     5881 catgatgata gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc
     5941 gatagtttac atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt
     6001 ttacagactg taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa
     6061 aatatcgtta ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg
     6121 cgagagcgta tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa
     6181 gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa
     6241 aatggaagag acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat
     6301 gtcgatcaca ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta
     6361 acgcgttctg ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa
     6421 aagatgaaaa actattggag acaacttcta aacgccaagt taatcactca acgtaagttt
     6481 gataatttaa cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc
     6541 aaacgccaat tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt
     6601 cgcatgaata ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc
     6661 ttaaaatcta aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag
     6721 attaacaatt accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg
     6781 attaagaaat atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat
     6841 gttcgtaaaa tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc
     6901 ttttactcta atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt
     6961 cgcaaacgcc ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg
     7021 cgagattttg ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa
     7081 acagaagtac agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac
     7141 aagcttattg ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca
     7201 acggtagctt attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta
     7261 aaatccgtta aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat
     7321 ccgattgact ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa
     7381 ctacctaaat atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc
     7441 ggagaattac aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat
     7501 ttagctagtc attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg
     7561 tttgtggagc agcataagca ttatttagat gagattattg agcaaatcag tgaattttct
     7621 aagcgtgtta ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat
     7681 agagacaaac caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat
     7741 cttggagctc ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg
     7801 tctacaaaag aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa
     7861 acacgcattg atttgagtca gctaggaggt gactaataac attggaagtg gataacggat
     7921 ccgcgatcgc ggcgcgccac ctggtggccg gccggtacca cgcgtgcgcg ctgatccggc
     7981 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     8041 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     8101 atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa
     8161 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg
     8221 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga
     8281 tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta
     8341 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     8401 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat
     8461 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
     8521 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8581 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8641 ctcatgagac aataaccctg ataaatgctt caataacatt gaaaaaggaa gagtatgagt
     8701 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8761 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8821 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8881 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     8941 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9001 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9061 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9121 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9181 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     9241 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9301 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9361 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9421 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9481 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9541 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9601 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9661 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9721 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9781 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9841 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     9901 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9961 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10021 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag ctt
//
