LOCUS       sequence-194633-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9 Cluster 2 variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC228 - Bacterial expression plasmid for SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     358..375
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    390..529
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(455..474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     615..637
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(631..822)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2280..2299
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2383..2402
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2383..2401
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2490..2512
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2532..2561
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2571..3671
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     3645..3668
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3726..3746
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3753..7859
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISAAAARFAASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7985..8003)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7999..8046
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8412..8417,8418..8434,8435..8440))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8467..8485)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8553..8657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8658..8726,8727..9518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8876..8895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9689..10073,1..204)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
       61 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      361 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
      421 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      481 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
      541 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      601 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      661 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
      721 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
      781 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
      841 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
      901 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
      961 atggatgcgg cgggaccaga gaaaaatcac tcagtgtcaa tgccagcgct tcgttaatac
     1021 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     1081 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     1141 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     1201 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     1261 cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg
     1321 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     1381 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     1441 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     1501 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
     1561 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
     1621 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     1681 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     1741 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
     1801 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     1861 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     1921 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     1981 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     2041 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc
     2101 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     2161 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     2221 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     2281 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     2341 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
     2401 ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat taagcggccg
     2461 ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga gatatacata
     2521 tgaaatcttc tcaccatcac catcaccatc accatcacca tggttcttct atgaaaatcg
     2581 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
     2641 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
     2701 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
     2761 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
     2821 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
     2881 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
     2941 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
     3001 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
     3061 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
     3121 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     3181 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     3241 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     3301 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
     3361 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact
     3421 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     3481 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc actatggaaa
     3541 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     3601 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     3661 acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga
     3721 tcgaggaaaa cctgtacttc caatccaatg caatggataa gaaatactca ataggcttag
     3781 atatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta
     3841 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg
     3901 ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta
     3961 gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga
     4021 tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag
     4081 acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg
     4141 agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg
     4201 atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga
     4261 ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac
     4321 aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag
     4381 cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc
     4441 ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc
     4501 ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata
     4561 cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt
     4621 ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta agagtaaata
     4681 ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc
     4741 aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa
     4801 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag
     4861 aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat
     4921 tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta
     4981 ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt
     5041 atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt
     5101 attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg
     5161 aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat
     5221 catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa
     5281 aacatagttt gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg
     5341 ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg
     5401 atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca
     5461 aaaaaataga atgttttgat agtgttgaaa tttcagctgc tgctgctaga tttgctgctt
     5521 cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg
     5581 aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg
     5641 agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac
     5701 agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta
     5761 ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca
     5821 atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa
     5881 aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta
     5941 gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag
     6001 taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa
     6061 ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag
     6121 aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa
     6181 agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata
     6241 ttaatcgttt aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg
     6301 attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg
     6361 ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca
     6421 agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg
     6481 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc
     6541 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta
     6601 ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt
     6661 tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa
     6721 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct
     6781 atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag
     6841 gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa
     6901 ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg
     6961 gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc
     7021 cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa
     7081 ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa
     7141 aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg
     7201 aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg
     7261 aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag
     7321 ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc
     7381 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa
     7441 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag
     7501 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta
     7561 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag
     7621 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta
     7681 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa
     7741 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc
     7801 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactaat
     7861 aacattggaa gtggataacg gatccgcgat cgcggcgcgc cacctggtgg ccggccggta
     7921 ccacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     7981 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     8041 tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat
     8101 cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg
     8161 gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag
     8221 cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg
     8281 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
     8341 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
     8401 taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
     8461 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8521 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8581 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataac
     8641 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8701 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8761 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8821 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8881 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8941 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9001 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9061 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9121 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9181 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9241 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9301 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9361 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9421 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9481 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     9841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cag
//
