LOCUS       sequence-194634-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC114 - Bacterial expression plasmid for SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     315..334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     568..585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    600..739
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(665..684)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     825..847
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(841..1032)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2490..2509
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2593..2612
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2593..2611
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2700..2722
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2742..2771
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2781..3881
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     3855..3878
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3936..3956
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3963..8069
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(8195..8213)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8209..8256
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8622..8627,8628..8644,8645..8650))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8677..8695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8763..8867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8868..8936,8937..9728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9086..9105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9899..10073,1..414)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
       61 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      181 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      601 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
      661 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
      721 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      781 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      841 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
      901 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
      961 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     1021 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     1081 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     1141 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     1201 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
     1261 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
     1321 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     1381 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     1441 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
     1501 ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     1561 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     1621 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     1681 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     1741 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     1801 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     1861 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     1921 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     1981 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     2041 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     2101 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     2161 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     2221 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     2281 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     2341 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
     2401 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     2461 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     2521 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
     2581 atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagtgccg
     2641 gctccggaga gctctttaat taagcggccg ccctgcagga ctcgagttct agaaataatt
     2701 ttgtttaact ttaagaagga gatatacata tgaaatcttc tcaccatcac catcaccatc
     2761 accatcacca tggttcttct atgaaaatcg aagaaggtaa actggtaatc tggattaacg
     2821 gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa
     2881 ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa
     2941 ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat
     3001 ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta
     3061 cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt
     3121 tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc
     3181 cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag
     3241 aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa
     3301 acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga
     3361 ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg
     3421 cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt
     3481 ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc
     3541 aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca
     3601 aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg
     3661 ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga
     3721 aagatccacg tattgccgcc actatggaaa acgcccagaa aggtgaaatc atgccgaaca
     3781 tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg
     3841 gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac taattcgagc tcgaacaaca
     3901 acaacaataa caataacaac aacctcggga tcgaggaaaa cctgtacttc caatccaatg
     3961 caatggataa gaaatactca ataggcttag atatcggcac aaatagcgtc ggatgggcgg
     4021 tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga aatacagacc
     4081 gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga gagacagcgg
     4141 aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtattt
     4201 gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc
     4261 gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg
     4321 gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa
     4381 aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc
     4441 atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg
     4501 atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc
     4561 ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa
     4621 gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga
     4681 atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag
     4741 aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg
     4801 cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta
     4861 ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc ctatcagctt
     4921 caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc
     4981 gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg
     5041 caggttatat tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt
     5101 tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa gatttgctgc
     5161 gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc
     5221 atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga
     5281 ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata
     5341 gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag
     5401 aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca aactttgata
     5461 aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg
     5521 tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc
     5581 tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa
     5641 ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat agtgttgaaa
     5701 tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa
     5761 ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg
     5821 ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg
     5881 ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg
     5941 gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat
     6001 tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg atccatgatg
     6061 atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt
     6121 tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga
     6181 ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg
     6241 ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc
     6301 gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc
     6361 ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa
     6421 gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatc
     6481 acattgttcc acaaagtttc cttgccgacg attcaataga caataaggtc ttaacgcgtt
     6541 ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc aaaaagatga
     6601 aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt
     6661 taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt atcaaacgcc
     6721 aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat agtcgcatga
     6781 atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat
     6841 ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca
     6901 attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct ttgattaaga
     6961 aatatccagc gcttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta
     7021 aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat ttcttttact
     7081 ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaag
     7141 cccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt
     7201 ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag
     7261 tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg gacaagctta
     7321 ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt ccaacggtag
     7381 cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg
     7441 ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa aatccgattg
     7501 actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt aaactaccta
     7561 aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt gccggagaat
     7621 tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta tatttagcta
     7681 gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa ttgtttgtgg
     7741 agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt tctaagcgtg
     7801 ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa catagagaca
     7861 aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg aatcttggag
     7921 ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat acgtctacaa
     7981 aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat gaaacacgca
     8041 ttgatttgag tcagctagga ggtgactaat aacattggaa gtggataacg gatccgcgat
     8101 cgcggcgcgc cacctggtgg ccggccggta ccacgcgtgc gcgctgatcc ggctgctaac
     8161 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
     8221 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
     8281 tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     8341 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     8401 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     8461 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     8521 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     8581 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat
     8641 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     8701 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8761 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8821 gacaataacc ctgataaatg cttcaataac attgaaaaag gaagagtatg agtattcaac
     8881 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8941 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9001 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9061 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     9121 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9181 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9241 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9301 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9361 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     9421 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9481 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9541 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9601 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9661 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9721 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9781 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9841 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10021 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc cac
//
