LOCUS       sequence-19469-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Kim HW, Ahn HJ, Yoon HJ, Kim HW, Baek SH, Suh SW
  TITLE     Crystallization and preliminary X-ray crystallographic analysis of 
            tRNA(m1G37)methyltransferase from Haemophilus influenzae.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 2003 Jan . 59(Pt 1):183-4.
  PUBMED    12499565
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr. 2003 Jan . 59(Pt 1):183-4."
COMMENT     Sequence Label: TrmD_HaeIn/pET28b(+) N,C-tag
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             854..871
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtacattccc tctanaataa ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccntcntcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tgtggatcgg
      121 ggtaatttca ttattccccg aaatgtttaa agcaattacg gaatttgggg ttacaggtag
      181 agccgtaaaa cataatcttc tgaaagttga atgttggaat ccaagagatt ttacattcga
      241 caagcataaa accgtggacg atcaccctta tggtggtggc ccaggaatgc taatgatggt
      301 gcaaccttta cgggatgcga ttcatactgc aaaagctgcg gcaggagaag gcgcaaaggt
      361 gatttacctt tcgccacaag gacgtaaact cgatcaaggc ggcgtaaccg agcttgctca
      421 aaatcagaaa ttgattttag tatgtggacg ttacgaaggg attgatgaac ggttgattca
      481 aactgaaatt gatgaagaat ggtcaatcgg cgattacgtt ctgaccggtg gggaattgcc
      541 ggcaatgaca ttaattgatg ctgttgcacg ttttattccc ggtgtattag gcaaacaagc
      601 ctcggcagaa gaagattctt ttgcagatgg tttattagat tgcccgcatt acactagacc
      661 agaagtgtta gaaggattaa ctgtaccacc cgtgctgatg tcgggacatc acgaagaaat
      721 tcggaaatgg cgattaaaac aatcgctaca gagaacgtgg ctccgacgcc ctgagctctt
      781 agaaggccta gctctgactg acgaacaacg taagctgtta aaagaggcgc aagccgagca
      841 taacagtctc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaa
//
