LOCUS       sequence-194694-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the 
            TEF1 promoter in yeast. Both cassettes are integrated in HO locus. 
            Assembly and characterization of the auxin-inducible degradation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.018"; journalName: "Mol Cell"; date: 
            "2017-01-19- 19"; volume: "65"; issue: "2"; pages: "285-295"
COMMENT     Sequence Label: pG2J
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(240..242,243..245,246..956)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(285..306)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(546..565)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     893..914
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga
       61 aaattttttt tttgattttt ttctctttcg atgacctccc attgatattt aagttaataa
      121 acggtcttca atttctcaag tttcagtttc atttttcttg ttctattaca acttttttta
      181 cttcttgctc attagaaaga aagcatagca atctaatcta agtttcctag gttattaaaa
      241 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      301 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      361 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      421 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      481 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      541 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      601 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      661 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      721 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      781 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      841 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      901 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggtcg
      961 acggtgcagg cgccggggct ggtgctgggg caggtacccc taaagatcca gccaaacctc
     1021 cggccaaggc acaagttgtg ggatggccac
//
