LOCUS       sequence-194696-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the 
            TEF1 promoter in yeast. Both cassettes are integrated in HO locus. 
            Assembly and characterization of the auxin-inducible degradation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.018"; journalName: "Mol Cell"; date: 
            "2017-01-19- 19"; volume: "65"; issue: "2"; pages: "285-295"
COMMENT     Sequence Label: pG2J
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(119..136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(119..135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(128..150)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(435..454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     567..588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(742..761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     861..883
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(921..939)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttaaaggac cagagtgtat aaaatgtggc ggaatcttac agtggcctat gcggccgctc
       61 tagctagaac tagtggatcg atccccaatt cgccctatag tgagtcgtat tacaattcac
      121 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      181 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      241 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      301 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
      361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      421 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      481 tccccgtcaa gctctaaatc ggggtgggcc atcgccctga tagacggttt ttcgcccttt
      541 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      601 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
      661 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
      721 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      781 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      841 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      901 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      961 aataatggtt tctta
//
