LOCUS       sequence-194697-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the 
            TEF1 promoter in yeast. Both cassettes are integrated in HO locus. 
            Assembly and characterization of the auxin-inducible degradation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.018"; journalName: "Mol Cell"; date: 
            "2017-01-19- 19"; volume: "65"; issue: "2"; pages: "285-295"
COMMENT     Sequence Label: pG2J
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      311..508
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg
       61 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
      121 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
      181 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
      241 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
      301 tctaatcagt actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
      361 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
      421 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
      481 tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt
      541 gtcgaaaacg agctcgaatt aattcctggg ggaacaactt cacagaatgt tttgtcatat
      601 tgtcgaagtg gtcacaaaac aagagaagtt ccgccaatta taaaaaggga acccgtatat
      661 ttcagcttca cggatgattt ccagggtgag agtactgtat atgggcttac gatagaaggc
      721 cataaaaatt tcttgcttgg caacaaaata gaagtgaaat catgtcgagg ctgctgtgtg
      781 ggagaacagc ataaaatatc acaaaaaaag aatctaaaac actgtgttgc ttgtcccaga
      841 aagggaatca agtattttta taaagattgg agtggtaaaa atcgagtatg tgctagatgc
      901 tatggaagat acaaattcag cggtcatcac tgtataaatt gcaagtatgt accagaagca
      961 cgtgaagtga aaaaggcaaa agacaaaggc gaaaaattgg gcattacgcc cgaaggtttg
     1021 ccagttaaag gaccagagtg tataaaatgt
//
