LOCUS       sequence-19471-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Han BW, Lee JY, Yang JK, Lee BI, Suh SW
  TITLE     Crystallization and preliminary X-ray crystallographic analysis of 
            deoxyuridine triphosphate nucleotidohydrolase from Saccharomyces 
            cerevisiae.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1147-9.
  PUBMED    11468401
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1147-9."
COMMENT     Sequence Label: pET-22b(+)-UTPase
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             480..497
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(550..568)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      564..611
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(735..754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgactgc tactagcgac aaagtactaa
       61 agattcaatt gcgctcagca agcgctactg tacctaccaa aggttctgcc actgccgcgg
      121 gatacgacat ttatgcatct caggatatta ccattccggc tatgggtcaa ggtatggttt
      181 ccaccgacat atcgttcacc gtacctgttg gtacctacgg tcgtattgcg ccaaggtcag
      241 gcctggcagt gaaaaacggt atccaaaccg gtgctggtgt tgtcgacaga gattacaccg
      301 gtgaagttaa agtagtttta ttcaatcgtt cacagaggga tttcgcgatc aaaaaaggtg
      361 atcgcgtagc ccaattgatt ctggaaaaaa ttgtcgatga tgcccagatc gttgttgtag
      421 actctctgga agaaagtgca agaggggccg gtggctttgg tagcactggt aacctcgagc
      481 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      541 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      601 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgcnncgt tcncngcttt
      781 cccgtcagct ctaaatcggg gc
//
