LOCUS       sequence-194722-        5900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5900)
  TITLE     SGC Toronto Q2 Q3 2016
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5TF2 (PREB)
FEATURES             Location/Qualifiers
     source          1..5900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1506..1640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1543..1562)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1597..1616
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1669..1834
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2018..2472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2105..2124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2314..2335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2498..2602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2603..2671,2672..3463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2821..2840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3634..4222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4123..4142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4376..4393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4527..4751
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4818..5351)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5521..5526,5527..5543,5544..5549))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag cagcggcaga
      181 gaaaacttgt atttccaggg catgggccgg cgccgggcgc cagagctgta ccgggctccg
      241 ttcccgttgt acgcgcttca ggtcgacccc agcactgggc tgctcatcgc tgcgggcgga
      301 ggaggcgccg ccaagacagg cataaagaat ggcgtgcact ttctgcagct agagctgatt
      361 aatgggcgct tgagtgcctc cttgctgcac tcccatgaca cagagacacg ggccaccatg
      421 aacttggcac tggctggtga catccttgct gcagggcagg atgcccactg tcagctcctg
      481 cgcttccagg cacatcaaca gcagggcaac aaggcagaga aggccggttc caaggagcag
      541 gggcctcgac aaaggaaggg agcagcccca gcagagaaga aatgtggagc ggaaacccag
      601 cacgaggggc tagaactcag ggtagagaat ttgcaggcgg tgcagacaga ctttagctcc
      661 gatccactgc agaaagttgt gtgcttcaac cacgataata ccctgcttgc cactggagga
      721 acagatggct acgtccgtgt ctggaaggtg cccagcctgg agaaggttct ggagttcaaa
      781 gcccacgaag gggagattga agacctggct ttagggcctg atggcaagtt ggtaaccgtg
      841 ggccgggacc ttaaggcctc tgtgtggcag aaggatcagc tggtgacaca gctgcactgg
      901 caagaaaatg gacccacctt ttccagcaca ccttaccgct accaggcctg caggtttggg
      961 caggttccag accagcctgc tggcctgcga ctcttcacag tgcaaattcc ccacaagcgc
     1021 ctgcgccagc cccctccctg ctacctcaca gcctgggatg gctccaactt cttgcccctt
     1081 cggaccaagt cctgtggcca tgaagtcgtc tcctgcctcg atgtcagtga atccggcacc
     1141 ttcctaggcc tgggcacagt cactggctct gttgccatct acatagcttt ctctctccag
     1201 tgcctctact acgtgaggga ggcccatggc attgtggtga cggatgtggc ctttctacct
     1261 gagaagggtc gtggtccaga gctccttggg tcccatgaaa ctgccctgtt ctctgtggct
     1321 gtggacagtc gttgccagct gcatctgttg ccctcacggc ggagtgtttg atgacgaagc
     1381 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     1441 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1501 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1561 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1621 tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt
     1681 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     1741 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     1801 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     1861 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     1921 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     1981 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     2041 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2101 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2161 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2221 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2281 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     2341 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2401 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2461 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2521 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2581 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2641 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2701 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2761 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2821 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2881 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2941 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3001 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3061 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3121 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3181 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3241 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3301 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3361 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3421 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3481 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3541 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3601 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3661 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3721 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3781 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3841 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3901 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3961 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4021 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4081 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4141 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4201 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4261 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4321 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4381 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     4441 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
     4501 ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
     4561 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
     4621 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     4681 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa
     4741 agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc
     4801 tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct
     4861 tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc
     4921 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgagcagatt gatgagcgcg
     4981 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca
     5041 ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct
     5101 tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg
     5161 gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca
     5221 agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc
     5281 atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     5341 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     5401 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     5461 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     5521 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     5581 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     5641 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     5701 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     5761 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     5821 gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacgt
     5881 atactccgga atattaatag
//
