LOCUS       sequence-194723-        6953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6953)
  TITLE     SGC Toronto Q2 Q3 2016
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5THA (Gemin5)
FEATURES             Location/Qualifiers
     source          1..6953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             187..204
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     polyA_signal    2559..2693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2596..2615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2650..2669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2722..2887
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3071..3525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3158..3177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3367..3388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3551..3655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3656..3724,3725..4516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3874..3893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4687..5275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5176..5195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5429..5446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5580..5804
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5871..6404)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6574..6579,6580..6596,6597..6602))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggcagcagcc atcatcatca tcatcacagc
      181 agcggcctgg ttccgcgtgg tagtatgggg caggagccgc ggacgctgcc gccctccccc
      241 aactggtact gcgcccgctg cagcgatgcc gtgcccgggg gcctctttgg cttcgccgcg
      301 cggacctccg tcttccttgt ccgcgtgggc ccgggcgcag gcgagagtcc agggacaccc
      361 ccgtttcgag tcataggaga gttggtggga cacaccgaaa gggtctctgg cttcacattt
      421 tctcatcacc ctggtcagta caacctctgt gccaccagct ccgacgatgg gactgtgaaa
      481 atatgggatg tagagacaaa aacagttgtg acagaacatg cactccatca gcatacgata
      541 tcaacattac attggtctcc tcgagtaaag gacttaatag tatctgggga tgaaaaagga
      601 gtagttttct gttactggtt taacagaaat gacagccagc acctctttat agaacccagg
      661 acaattttct gtcttacttg ttcacctcat catgaagatt tagtagccat tggctacaag
      721 gatggcatag tggtgataat tgacatcagt aagaaaggag aagttattca taggcttcga
      781 ggccatgatg atgaaatcca ctccatagcc tggtgtcccc tgcctggtga agattgttta
      841 tctataaacc aagaggaaac ttcagaagaa gctgaaatta ccaacgggaa tgctgtagca
      901 caagctccag taacaaaagg ttgctactta gccactggaa gcaaagatca aaccattcga
      961 atctggagct gttctagagg ccgaggggtg atgattttga aattgccctt tctgaagaga
     1021 agaggagggg gtatagaccc aactgttaaa gagcgccttt ggttgacact ccattggccc
     1081 agcaatcaac caacacagct ggtatctagc tgttttggag gtgaactgtt gcaatgggat
     1141 ctcactcaat cttggagacg gaaatacacc ctcttcagtg cctcatcaga agggcaaaat
     1201 cattcaagaa ttgtgtttaa tttatgtcct ttacaaacag aggatgacaa acagctgtta
     1261 ctttctacat caatggatag agatgtaaaa tgttgggaca tagccacctt ggagtgcagc
     1321 tggacccttc cttcccttgg tgggtttgca tacagcctgg ctttctcttc tgtggacata
     1381 ggctctttgg ccataggtgt tggggatggc atgatccgtg tatggaatac actctccata
     1441 aagaacaact atgatgtgaa aaatttttgg caaggcgtga agtccaaggt tacagcgctg
     1501 tgctggcacc caaccaagga aggttgctta gcttttggaa ctgatgatgg aaaagtggga
     1561 ttgtatgaca cctactccaa caagcctcca cagatttcta gcacatatca taagaagact
     1621 gtatatacgt tagcctgggg gccaccagta ccccccatgt cacttggagg agaaggagac
     1681 agaccttccc ttgctttata cagctgtgga ggagaaggga ttgtcttaca gcataatccc
     1741 tggaagctta gtggagaagc ctttgacatc aacaaactca tcagggacac caattcaatc
     1801 aaatacaaat tgcctgtaca cacagagata agttggaaag cagatggcaa aatcatggct
     1861 cttggcaatg aagatggatc aatagaaata tttcagattc ccaacctgaa actgatctgt
     1921 actatccaac agcatcacaa gcttgtgaat accattagct ggcatcatga gcatggcagc
     1981 cagccagaat tgagctatct gatggcctct ggctccaaca atgcagtcat ttacgtgcac
     2041 aacctgaaga ctgtcataga gagcagccct gagtctccag tgaccattac agagccctac
     2101 cggaccctct cagggcatac ggccaagatt accagtgtgg cgtggagccc acatcatgat
     2161 ggaaggctgg tatctgcttc ctatgatggt acagcccagg tgtgggatgc tctccgggaa
     2221 gagcccctgt gcaatttccg aggacatcaa ggtcgactgc tttgtgtggc atggtctcct
     2281 ttggatccag actgcatcta ttcaggggca gatgactttt gtgtgcacaa gtggctcact
     2341 tccatgcaag atcattcccg gcctcctcaa ggcaaaaaaa gtattgaatt ggagaaaaaa
     2401 cggctctctc aacctaaggc atgatgacga agcttgtcga gaagtactag aggatcataa
     2461 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2521 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2581 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2641 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
     2701 tgatatcgcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
     2761 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     2821 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     2881 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
     2941 tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc
     3001 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat
     3061 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3121 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3181 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3241 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3301 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3361 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3421 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3481 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     3541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3841 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3901 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3961 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4021 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4081 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4141 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4201 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4261 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4321 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4381 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4441 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4501 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4561 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4621 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4681 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4741 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4801 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4861 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4921 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5041 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5161 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5281 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5341 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5401 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     5461 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc
     5521 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt
     5581 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     5641 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     5701 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     5761 tattaaagag gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat
     5821 ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg
     5881 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga
     5941 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg
     6001 ttggcctcat gcttgagcag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg
     6061 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga
     6121 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat
     6181 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg
     6241 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg
     6301 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta
     6361 gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     6421 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     6481 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     6541 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     6601 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     6661 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
     6721 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
     6781 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
     6841 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
     6901 gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa tag
//
