LOCUS       sequence-194727-        5876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5876)
  TITLE     SGC Toronto Q2 Q3 2016
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5TEY (METTL14)
FEATURES             Location/Qualifiers
     source          1..5876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1482..1616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1519..1538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1573..1592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1645..1810
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1994..2448
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2081..2100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2290..2311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2474..2578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2579..2647,2648..3439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2797..2816)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3610..4198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4099..4118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4352..4369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4503..4727
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4794..5327)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5497..5502,5503..5519,5520..5525))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg gatagccgct tgcaggagat ccgggagcgg
      181 cagaagttac ggcgacagct cctcgcgcag cagttgggag ctgaaagtgc cgacagcatt
      241 ggtgccgtgt taaatagcaa agatgagcag agagaaattg ctgaaacaag agaaacttgc
      301 agggcttcct atgatacctc tgctccaaat gcaaaacgta agtatctgga tgaaggagag
      361 acagatgaag acaaaatgga agaatataag gatgaactag aaatgcaaca ggatgaagaa
      421 aatttgccat atgaagaaga gatttacaaa gattctagta cttttcttaa gggaacacag
      481 agcttaaatc cccataatga ttactgccaa cattttgtag acactggaca tagacctcag
      541 aatttcatca gggatgtagg tttagctgac agatttgaag aatatcctaa actgagggag
      601 ctcatcaggc taaaggatga gttaatagct aaatctaaca ctcctcccat gtacttacaa
      661 gccgatatag aagcctttga catcagagaa ctaacaccca aatttgatgt gattcttctg
      721 gaaccccctt tagaagaata ttacagagaa actggcatca ctgctaatga aaaatgctgg
      781 acttgggatg atattatgaa gttagaaatt gatgagattg cagcacctcg atcatttatt
      841 tttctctggt gtggttctgg ggaggggttg gaccttggaa gagtgtgttt acgaaaatgg
      901 ggttacagaa gatgtgaaga tatttgttgg attaaaacca ataaaaacaa tcctgggaag
      961 actaagactt tagatccaaa ggctgtcttt cagagaacaa aggaacactg cctcatgggg
     1021 atcaaaggaa ctgtgaagcg tagcacagac ggggacttca ttcatgctaa tgttgacatt
     1081 gacttaatta tcacagaaga acctgaaatt ggcaatatag aaaaacctgt agaaattttt
     1141 catataattg agcatttttg tcttggtaga agacgccttc atctatttgg aagagatagt
     1201 acaattcgac caggctggct cacagttgga ccaacgctta caaatagcaa ctacaatgca
     1261 gaaacatatg catcctattt cagtgctcct aattcctact tgactggttg tacagaagaa
     1321 attgagagac ttcgaccaaa atcgtgatga cgaagcttgt cgagaagtac tagaggatca
     1381 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     1441 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     1501 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1561 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     1621 cactgatatc gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
     1681 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     1741 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     1801 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
     1861 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
     1921 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
     1981 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2041 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2101 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2161 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2221 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2281 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     2341 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2401 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     2461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2581 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2641 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2701 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2761 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2821 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2881 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2941 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3001 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3061 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3121 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3181 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3241 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3301 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3361 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3421 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4261 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4321 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4381 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     4441 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     4501 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     4561 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     4621 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     4681 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     4741 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     4801 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     4861 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     4921 gcgttggcct catgcttgag cagattgatg agcgcggtgg caatgccctg cctccggtgc
     4981 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     5041 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     5101 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     5161 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     5221 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     5281 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     5341 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     5401 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     5461 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     5521 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     5581 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     5641 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     5701 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     5761 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     5821 caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat taatag
//
