LOCUS       sequence-194730-        6473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6473)
  TITLE     SGC Toronto Q2 Q3 2016
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5TEY (METTL3)
FEATURES             Location/Qualifiers
     source          1..6473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2079..2213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2116..2135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2170..2189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2242..2407
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2591..3045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2678..2697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2887..2908
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3071..3175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3176..3244,3245..4036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3394..3413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4207..4795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4696..4715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4949..4966
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5100..5324
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5391..5924)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6094..6099,6100..6116,6117..6122))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag cagcggcaga
      181 gaaaacttgt atttccaggg catgtcggac acgtggagct ctatccaggc ccacaagaag
      241 cagctggact ctctgcggga gaggctgcag cggaggcgga agcaggactc ggggcacttg
      301 gatctacgga atccagaggc agcattgtct ccaaccttcc gtagtgacag cccagtgcct
      361 actgcaccca cctctggtgg ccctaagccc agcacagctt cagcagttcc tgaattagct
      421 acagatcctg agttagagaa gaagttgcta caccacctct ctgatctggc cttaacattg
      481 cccactgatg ctgtgtccat ctgtcttgcc atctccacgc cagatgctcc tgccactcaa
      541 gatggggtag aaagcctcct gcagaagttt gcagctcagg agttgattga ggtaaagcga
      601 ggtctcctac aagatgatgc acatcctact cttgtaacct atgctgacca ttccaagctc
      661 tctgccatga tgggtgctgt ggcagaaaag aagggccctg gggaggtagc agggactgtc
      721 acagggcaga agcggcgtgc agaacaggac tcgactacag tagctgcctt tgccagttcg
      781 ttagtctctg gtctgaactc ttcagcatcg gaaccagcaa aggagccagc caagaaatca
      841 aggaaacatg ctgcctcaga tgttgatctg gagatagaga gccttctgaa ccaacagtcc
      901 actaaggaac aacagagcaa gaaggtcagt caggagatcc tagagctatt aaatactaca
      961 acagccaagg aacaatccat tgttgaaaaa tttcgctctc gaggtcgggc ccaagtgcaa
     1021 gaattctgtg actatggaac caaggaggag tgcatgaaag ccagtgatgc tgatcgaccc
     1081 tgtcgcaagc tgcacttcag acgaattatc aataaacaca ctgatgagtc tttaggtgac
     1141 tgctctttcc ttaatacatg tttccacatg gatacctgca agtatgttca ctatgaaatt
     1201 gatgcttgca tggattctga ggcccctggc agcaaagacc acacgccaag ccaggagctt
     1261 gctcttacac agagtgtcgg aggtgattcc agtgcagacc gactcttccc acctcagtgg
     1321 atctgttgtg atatccgcta cctggacgtc agtatcttgg gcaagtttgc agttgtgatg
     1381 gctgacccac cctgggatat tcacatggaa ctgccctatg ggaccctgac agatgatgag
     1441 atgcgcaggc tcaacatacc cgtactacag gatgatggct ttctcttcct ctgggtcaca
     1501 ggcagggcca tggagttggg gagagaatgt ctaaacctct gggggtatga acgggtagat
     1561 gaaattattt gggtgaagac aaatcaactg caacgcatca ttcggacagg ccgtacaggt
     1621 cactggttga accatgggaa ggaacactgc ttggttggtg tcaaaggaaa tccccaaggc
     1681 ttcaaccagg gtctggattg tgatgtgatc gtagctgagg ttcgttccac cagtcataaa
     1741 ccagatgaaa tctatggcat gattgaaaga ctatctcctg gcactcgcaa gattgagtta
     1801 tttggacgac cacacaatgt gcaacccaac tggatcaccc ttggaaacca actggatggg
     1861 atccacctac tagacccaga tgtggttgca cggttcaagc aaaagtaccc agatggtatc
     1921 atctctaaac ctaagaattt atgatgacga agcttgtcga gaagtactag aggatcataa
     1981 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2041 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2101 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2161 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
     2221 tgatatcgcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
     2281 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     2341 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     2401 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
     2461 tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc
     2521 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat
     2581 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2881 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2941 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3001 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     3061 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3121 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3181 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3241 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3301 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3361 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3421 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3481 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3541 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3601 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3661 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3721 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3781 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3841 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3901 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3961 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4021 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4081 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4141 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4201 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4261 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4321 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4381 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4441 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4501 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4561 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4621 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4681 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4741 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4801 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4861 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4921 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4981 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc
     5041 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt
     5101 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     5161 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     5221 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     5281 tattaaagag gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat
     5341 ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg
     5401 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga
     5461 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg
     5521 ttggcctcat gcttgagcag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg
     5581 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga
     5641 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat
     5701 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg
     5761 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg
     5821 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta
     5881 gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     5941 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     6001 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     6061 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     6121 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     6181 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
     6241 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
     6301 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
     6361 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
     6421 gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa tag
//
