LOCUS       sequence-194732-        9963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon system construct with 10 concatenated U6 
            promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4,
            SOX2 and LIN28A promoters. Contains PGK-puro selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: PB-GG-OMKSL-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   2240..2302
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     2515..2531
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2532..2780
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2801..2876
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     2908..2924
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2925..3173
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3195..3270
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(3282..3298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3302..3318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3319..3567
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3589..3664
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3696..3712
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3713..3961
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3983..4058
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4090..4106
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4107..4355
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4377..4452
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    4610..4643
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4985..5001
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5002..5250
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        5272..5347
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     5379..5395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5396..5644
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        5666..5741
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(5753..5769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5773..5789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5790..6038
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6060..6135
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     6167..6183
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6184..6432
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6454..6529
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     6561..6577
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6578..6826
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        6848..6923
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7081..7114
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7175..7672
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     7605..7624
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7712..8311
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7712..7731)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8208..8228
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8392..8409)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8398..8622
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8669..8685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8693..8709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8717..8723,8724..8741,8742..8747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8762..8783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(8842..8966)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     repeat_region   9256..9290
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9319..9338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9320..9338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9348..9364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9357..9379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9506..9961
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9593..9612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9803..9824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggattaatta agcggccgca
     2221 tacgcgtata ctagattaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
     2281 tcatgcgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
     2341 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
     2401 caatttattt atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata
     2461 aagtaacaaa acttttaact agaggatctc gagctcaagc ttcgaattcc ccctgtaaaa
     2521 cgacggccag tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     2581 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     2641 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     2701 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2761 tgtggaaagg acgaaacacc ggggggagaa actgaggcga gttttagagc tagaaatagc
     2821 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     2881 tttcatggtc atagctgttt cctcatggta aaacgacggc cagtgagggc ctatttccca
     2941 tgattccttc atatttgcat atacgataca aggctgttag agagataatt ggaattaatt
     3001 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     3061 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     3121 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcccttt
     3181 ataatgcgag ggtcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     3241 caacttgaaa aagtggcacc gagtcggtgc tttttttcat ggtcatagct gtttcctgga
     3301 cgtaaaacga cggccagtga gggcctattt cccatgattc cttcatattt gcatatacga
     3361 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     3421 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     3481 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     3541 tatatcttgt ggaaaggacg aaacaccggc tgccatagca acgatggagt tttagagcta
     3601 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     3661 gtgctttttt tcatggtcat agctgtttcc tccaggtaaa acgacggcca gtgagggcct
     3721 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattgg
     3781 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     3841 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     3901 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     3961 cgggcacagt gccagaggtc tggttttaga gctagaaata gcaagttaaa ataaggctag
     4021 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttcatgg tcatagctgt
     4081 ttccttgttg taaaacgacg gccagtgagg gcctatttcc catgattcct tcatatttgc
     4141 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     4201 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     4261 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     4321 tggctttata tatcttgtgg aaaggacgaa acaccgtctg tgggggacct gcactggttt
     4381 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     4441 ccgagtcggt gctttttttc atggtcatag ctgtttccta acgagcggcc gcaggaaccc
     4501 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4561 ccaaaggtcg cccgacgccc aattccgatc atattcaata acccttaata taacttcgta
     4621 taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagcttag
     4681 gatctcgacc tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     4741 atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     4801 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     4861 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     4921 acgtgacaaa tggaagtagc acgtctcact agaggatctc gagctcaagc ttcgaattcc
     4981 ccctgtaaaa cgacggccag tgagggccta tttcccatga ttccttcata tttgcatata
     5041 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
     5101 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     5161 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     5221 ttatatatct tgtggaaagg acgaaacacc ggtgtcagag accggagttg tgttttagag
     5281 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     5341 tcggtgcttt ttttcatggt catagctgtt tcctcatggt aaaacgacgg ccagtgaggg
     5401 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     5461 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     5521 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     5581 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     5641 caccggtggc tggcaggctg gctctgtttt agagctagaa atagcaagtt aaaataaggc
     5701 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc
     5761 tgtttcctgg acgtaaaacg acggccagtg agggcctatt tcccatgatt ccttcatatt
     5821 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     5881 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     5941 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     6001 tcttggcttt atatatcttg tggaaaggac gaaacaccgc ggggtactca agtcttctag
     6061 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     6121 gcaccgagtc ggtgcttttt ttcatggtca tagctgtttc ctccaggtaa aacgacggcc
     6181 agtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     6241 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     6301 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     6361 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     6421 ggacgaaaca ccgtaattat ctgcccgggg ggtgttttag agctagaaat agcaagttaa
     6481 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcatg
     6541 gtcatagctg tttccttgtt gtaaaacgac ggccagtgag ggcctatttc ccatgattcc
     6601 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
     6661 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     6721 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     6781 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgcaa aacccggcag
     6841 cgaggctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     6901 aaaaagtggc accgagtcgg tgcttttttt catggtcata gctgtttcct aacgagcggc
     6961 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     7021 aggccgggcg accaaaggtc gcccgacgcc caattccgat catattcaat aacccttaat
     7081 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca
     7141 gccaagctta ggatctcgac ctcgaaattc taccgggtag gggaggcgct tttcccaagg
     7201 cagtctggag catgcgcttt agcagccccg ctggcacttg gcgctacaca agtggcctct
     7261 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     7321 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     7381 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     7441 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     7501 ccttcgcttt ctgggctcag cagctgggaa gggtgggtcc gggggcgggc tcaggggcgg
     7561 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     7621 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcat
     7681 ccatctagat ctcgagcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct
     7741 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     7801 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     7861 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7921 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7981 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     8041 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     8101 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     8161 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     8221 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     8281 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     8341 cgaaaggagc gcacgacccc atgcatcgat gatctagagc tcgctgatca gcctcgactg
     8401 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8461 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8521 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8581 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     8641 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8821 acctgtcgtg ccagcggatc ccatagtgac tggatatgtt gtgttttaca gtattatgta
     8881 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     8941 cgttcagctt ttttgtacaa acttgtgcta gcattctagt tgatatctat aacaagaaaa
     9001 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     9061 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     9121 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     9181 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     9241 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctaga
     9301 atggcgcgcc ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     9361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9481 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9841 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9901 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9961 tag
//
