LOCUS       sequence-194733-       17210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for CAG promoter constitutive expression of 
            dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53
            shRNA expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17210)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 17210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: pCXLE-dCas9VPH-T2A-GFP-shP53
FEATURES             Location/Qualifiers
     source          1..17210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1752..1778
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1782..1802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1812..1835,1836..1838,1839..4394,4395..4397,
                     4398..5912)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1812..1835,1836..1838,1839..4325,4326..4328,
                     4329..5912)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5916..5936
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5973..6083
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6135..6245
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6297..6407
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6459..6569
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             6618..7160
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             7185..7556
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             7578..7631
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7638..7640,7641..7643,7644..8357)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7638..7640,7641..7643,7644..8354)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7944..7963)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8291..8312
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8395..8983
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8448..8468)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8866..8877)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9073..9092)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9138..9193
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9192..9211)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9554..9570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9554..9570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9567..9589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9578..9594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9602..9608,9609..9626,9627..9632))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9647..9668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    9757..9790
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             10251..12176
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    12344..12377
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      12564..14353
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        14824..15137
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     15056..15076
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(15288..15305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15459..16047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15539..15558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16218..17009,17010..17078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16841..16860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17079..17183)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgaccg
     1741 gtgccaccat gtacccatac gatgttccag attacgcttc gccgaagaaa aagcgcaagg
     1801 tcgaagcgtc cgacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct
     1861 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     1921 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     1981 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     2041 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     2101 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     2161 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     2221 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     2281 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     2341 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     2401 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     2461 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     2521 tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     2581 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     2641 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     2701 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     2761 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
     2821 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     2881 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     2941 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     3001 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
     3061 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     3121 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     3181 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     3241 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     3301 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     3361 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     3421 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     3481 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     3541 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     3601 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     3661 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     3721 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     3781 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     3841 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     3901 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     3961 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     4021 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     4081 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     4141 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     4201 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     4261 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     4321 tcgacgccat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga
     4381 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     4441 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     4501 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     4561 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     4621 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     4681 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     4741 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     4801 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     4861 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     4921 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     4981 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
     5041 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
     5101 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
     5161 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
     5221 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     5281 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     5341 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     5401 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
     5461 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     5521 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     5581 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
     5641 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
     5701 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     5761 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
     5821 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     5881 cacggatcga cctgtctcag ctgggaggcg acagccccaa gaagaagaga aaggtggagg
     5941 ccagcctcga gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca
     6001 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     6061 acgattttga ccttgacatg ctccccgggt cactacccag cgctagcgtc gagtgcgaaa
     6121 gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     6181 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     6241 tgctccccgg gtcactaccc agcgctagcg tcgagtgcga aagcggcggg ccggccgacg
     6301 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     6361 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtcactac
     6421 ccagcgctag cgtcgagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact
     6481 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     6541 ctcttgacga ttttgacctt gacatgctcc ccgggtcact acccagcgct agcgtcgagt
     6601 tcgaaggcag cggcgggcct tcagggcaga tcagcaacca ggccctggct ctggccccta
     6661 gctccgctcc agtgctggcc cagactatgg tgccctctag tgctatggtg cctctggccc
     6721 agccacctgc tccagcccct gtgctgaccc caggaccacc ccagtcactg agcgctccag
     6781 tgcccaagtc tacacaggcc ggcgagggga ctctgagtga agctctgctg cacctgcagt
     6841 tcgacgctga tgaggacctg ggagctctgc tggggaacag caccgatccc ggagtgttca
     6901 cagatctggc ctccgtggac aactctgagt ttcagcagct gctgaatcag ggcgtgtcca
     6961 tgtctcatag tacagccgaa ccaatgctga tggagtaccc cgaagccatt acccggctgg
     7021 tgaccggcag ccagcggccc cccgaccccg ctccaactcc cctgggaacc agcggcctgc
     7081 ctaatgggct gtccggagat gaagacttct caagcatcgc tgatatggac tttagtgccc
     7141 tgctgtcaca gatttcctct agtgggcagg gaggaggtgg aagcggcttc agcgtggaca
     7201 ccagtgccct gctggacctg ttcagcccct cggtgaccgt gcccgacatg agcctgcctg
     7261 accttgacag cagcctggcc agtatccaag agctcctgtc tccccaggag ccccccaggc
     7321 ctcccgaggc agagaacagc agcccggatt cagggaagca gctggtgcac tacacagcgc
     7381 agccgctgtt cctgctggac cccggctccg tggacaccgg gagcaacgac ctgccggtgc
     7441 tgtttgagct gggagagggc tcctacttct ccgaagggga cggcttcgcc gaggacccca
     7501 ccatctccct gctgacaggc tcggagcctc ccaaagccaa ggaccccact gtctcctcta
     7561 gcgtcgacgg ctccggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga
     7621 atcctggccc aatcgatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     7681 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     7741 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     7801 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     7861 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     7921 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     7981 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     8041 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     8101 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     8161 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     8221 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     8281 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     8341 acgagctgta caagtaattt aaagaattca agcttcgggg actagtcata tgataatcaa
     8401 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8461 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8521 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8581 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8641 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8701 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8761 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     8821 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8881 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8941 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggta aattcactcc
     9001 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     9061 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     9121 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     9181 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     9241 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     9301 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     9361 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     9421 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     9481 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     9541 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9601 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9661 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9721 ggatctcaat tccgatcata ttcaataacc cttaatataa cttcgtataa tgtatgctat
     9781 acgaagttat taggtctgaa gaggagttta cgtccagcca agcttaggat caattctcat
     9841 gtttgacagc ttatcatcga taagctgatc ctcacaggcc gcacccagct tttcttccgt
     9901 tgccccagta gcatctctgt ctggtgacct tgaagaggaa gaggaggggt cccgagaatc
     9961 cccatcccta ccgtccagca aaaaggggga cgaggaattt gaggcctggc ttgaggctca
    10021 ggacgcaaat cttgaggatg ttcagcggga gttttccggg ctgcgagtaa ttggtgatga
    10081 ggacgaggat ggttcggagg atggggaatt ttcagacctg gatctgtctg acagcgacca
    10141 tgaaggggat gagggtgggg gggctgttgg agggggcagg agtctgcact ccctgtattc
    10201 actgagcgtc gtctaataaa gatgtctatt gatctctttt agtgtgaatc atgtctgacg
    10261 aggggccagg tacaggacct ggaaatggcc taggagagaa gggagacaca tctggaccag
    10321 aaggctccgg cggcagtgga cctcaaagaa gagggggtga taaccatgga cgaggacggg
    10381 gaagaggacg aggacgagga ggcggaagac caggagcccc gggcggctca ggatcagggc
    10441 caagacatag agatggtgtc cggagacccc aaaaacgtcc aagttgcatt ggctgcaaag
    10501 ggacccacgg tggaacagga gcaggagcag gagcgggagg ggcaggagca ggaggggcag
    10561 gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagggg
    10621 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
    10681 caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga ggaggggcag
    10741 gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg
    10801 caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag
    10861 caggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca ggaggagggg
    10921 caggaggggc aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag
    10981 gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag
    11041 gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg
    11101 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag
    11161 gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag
    11221 caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga ggtagtggag
    11281 gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga gccagggggg
    11341 gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg cccaggagtc
    11401 ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca ggtagaaggc
    11461 catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa ggtggcccag
    11521 atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat gacccaggag
    11581 aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa aaaggagggt
    11641 ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac attgcagaag
    11701 gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa ggaacttggg
    11761 tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta aggcgaggaa
    11821 ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc tttggaatgg
    11881 cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt tatttcatgg
    11941 tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag gaccttgtta
    12001 tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt gacgatggag
    12061 tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag ggtgatgacg
    12121 gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag gagtgatgta
    12181 acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc actaaagaat
    12241 aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc
    12301 attttcgtcc tcccaacatg gggcaattgc cggaaccctt aatataactt cgtataatgt
    12361 atgctatacg aagttattag gtccctcgaa gaggttcact agcggatctc aattgggcat
    12421 acccatgttg tcacgtcact cagctccgcg ctcaacacct tctcgcgttg gaaaacatta
    12481 gcgacattta cctggtgagc aatcagacat gcgacggctt tagcctggcc tccttaaatt
    12541 cacctaagaa tgggagcaac cagcaggaaa aggacaagca gcgaaaattc acgccccctt
    12601 gggaggtggc ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct
    12661 gactgtatat gcatgaggat agcatatgct acccggatac agattaggat agcatatact
    12721 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
    12781 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct
    12841 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct
    12901 acccagatat agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc
    12961 agatataaat taggatagca tatactaccc taatctctat taggatagca tatgctaccc
    13021 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
    13081 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
    13141 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
    13201 agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg
    13261 caacattagc ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc
    13321 ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta
    13381 tcttggcccg cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg
    13441 gcaagtggtt tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta
    13501 ttttacagtc caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa
    13561 tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg
    13621 tttaattttc gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct
    13681 ctttcccctt gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg
    13741 ggacacatct taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca
    13801 taaattcgtg tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta
    13861 ttgttaaaga tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg
    13921 tgagggttat attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat
    13981 tctgggggcg tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca
    14041 actcagcagt tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg
    14101 agagttcact gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact
    14161 accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat
    14221 atcgctgttc cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg
    14281 gtaacatatg ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca
    14341 tatgctaccc gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt
    14401 gagggtccgc ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg
    14461 tagtcttcct gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc
    14521 aagagttaca cataaaggca atgttgtgtt gcagtccaca gactgcaaag tctgctccag
    14581 gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt tataaggatg tcaactacag
    14641 tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg
    14701 gcaagttgta ccaaccaact gaagggatta catgcactgc cccgcgaaga aggggcagag
    14761 atgtcgtagt caggtttagt tcgtccgggg cggggcatcg atcctctaga gtcgacgcta
    14821 gcggatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga cttgtgggag
    14881 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
    14941 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
    15001 tgataggctt ggatttctat aagagataca aatactaaat tattatttta aaaaacagca
    15061 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaatatccct
    15121 tggagaaaag ccttgtttgg gccgactcca gtggtaatct acttcaagag agtagattac
    15181 cactggagtc ttttttggaa ttcctgcagc ccgggggatc cgctgcatta atgaatcggc
    15241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    15301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    15361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    15421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    15481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    15541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    15601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    15661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    15721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    15781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    15841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    15901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    15961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    16021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    16081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    16141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    16201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    16261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    16321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    16381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    16441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    16501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    16561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    16621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    16681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    16741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    16801 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    16861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    16921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    16981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    17041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    17101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    17161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
//
