LOCUS       sequence-194736-       10912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs 
            targeting EEA-motif. Includes PGK-puro selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: GG-EBNA-EEA-5guides-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..10912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(190..209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     309..331
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(369..387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        455..559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(560..628,629..1420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(778..797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1591..2179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2080..2099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2467..2488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2503..2508,2509..2526,2527..2533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2541..2557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2546..2568
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2565..2581
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2600..2619
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2600..2618
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2685..2701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2702..2950
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2966..3041
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3073..3089
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3090..3338
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3354..3429
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(3441..3457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3461..3477
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3478..3726
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3742..3817
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3849..3865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3866..4114
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4130..4205
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4237..4253
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4254..4502
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4518..4593
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    4751..4784
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4845..5342
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4880..4903)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5275..5294
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5382..5981
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5382..5401)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5878..5898
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6430..8355
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    8523..8556
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      8743..10532
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg
       61 ggcatcgatc ctctagtaag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      121 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
      181 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      241 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      301 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      361 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      421 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      541 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      601 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      661 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      721 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      781 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      841 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      901 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      961 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1021 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1081 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1141 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1201 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1261 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1321 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1381 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1441 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1501 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1561 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2161 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2221 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2281 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2341 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2401 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2461 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2521 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2581 ccatgattac gccaagctct aatacgactc actataggga gacaagcttg catgcctgca
     2641 ggtcgactct agaggatctc gagctcaagc ttcgaattcc ccctgtaaaa cgacggccag
     2701 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2761 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2821 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     2881 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2941 acgaaacacc gcccagcact ttggggtttt agagctagaa atagcaagtt aaaataaggc
     3001 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc
     3061 tgtttcctca tggtaaaacg acggccagtg agggcctatt tcccatgatt ccttcatatt
     3121 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     3181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     3241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     3301 tcttggcttt atatatcttg tggaaaggac gaaacaccga atcccagcac tttgttttag
     3361 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     3421 agtcggtgct ttttttcatg gtcatagctg tttcctggac gtaaaacgac ggccagtgag
     3481 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     3541 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     3601 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     3661 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     3721 aacaccggcc tcccaaagtg cgttttagag ctagaaatag caagttaaaa taaggctagt
     3781 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttcatggt catagctgtt
     3841 tcctccaggt aaaacgacgg ccagtgaggg cctatttccc atgattcctt catatttgca
     3901 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
     3961 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
     4021 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
     4081 ggctttatat atcttgtgga aaggacgaaa caccggctac ttgggaggcg ttttagagct
     4141 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     4201 ggtgcttttt ttcatggtca tagctgtttc cttgttgtaa aacgacggcc agtgagggcc
     4261 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     4321 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     4381 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     4441 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     4501 ccggcctccc aagtagcgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     4561 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt catggtcata gctgtttcct
     4621 aacgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4681 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc caattccgat catattcaat
     4741 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag
     4801 tttacgtcca gccaagctta ggatctcgac ctcgaaattc taccgggtag gggaggcgct
     4861 tttcccaagg cagtctggag catgcgcttt agcagccccg ctggcacttg gcgctacaca
     4921 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4981 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     5041 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     5101 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     5161 cagctttgct ccttcgcttt ctgggctcag cagctgggaa gggtgggtcc gggggcgggc
     5221 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     5281 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     5341 cgacctgcat ccatctagat ctcgagcagc tgaagcttac catgaccgag tacaagccca
     5401 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5461 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5521 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5581 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5641 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5701 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5761 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5821 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5881 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5941 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     6001 agcgcccgac cgaaaggagc gcacgacccc atgcatcgat aagctgatcc tcacaggccg
     6061 cacccagctt ttcttccgtt gccccagtag catctctgtc tggtgacctt gaagaggaag
     6121 aggaggggtc ccgagaatcc ccatccctac cgtccagcaa aaagggggac gaggaatttg
     6181 aggcctggct tgaggctcag gacgcaaatc ttgaggatgt tcagcgggag ttttccgggc
     6241 tgcgagtaat tggtgatgag gacgaggatg gttcggagga tggggaattt tcagacctgg
     6301 atctgtctga cagcgaccat gaaggggatg agggtggggg ggctgttgga gggggcagga
     6361 gtctgcactc cctgtattca ctgagcgtcg tctaataaag atgtctattg atctctttta
     6421 gtgtgaatca tgtctgacga ggggccaggt acaggacctg gaaatggcct aggagagaag
     6481 ggagacacat ctggaccaga aggctccggc ggcagtggac ctcaaagaag agggggtgat
     6541 aaccatggac gaggacgggg aagaggacga ggacgaggag gcggaagacc aggagccccg
     6601 ggcggctcag gatcagggcc aagacataga gatggtgtcc ggagacccca aaaacgtcca
     6661 agttgcattg gctgcaaagg gacccacggt ggaacaggag caggagcagg agcgggaggg
     6721 gcaggagcag gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg
     6781 gcaggagggg caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga
     6841 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
     6901 ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
     6961 ggaggagggg caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga
     7021 gcaggaggag gggcaggagc aggaggggca ggaggggcag gaggggcagg agcaggaggg
     7081 gcaggagcag gaggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca
     7141 ggaggggcag gagcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg
     7201 gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
     7261 gcaggagcag gaggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca
     7321 ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg aggaggggca
     7381 ggagcaggag gggcaggagc aggaggtgga ggccggggtc gaggaggcag tggaggccgg
     7441 ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg gaggccgccg gggtagagga
     7501 cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg tggacgtgga
     7561 gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc accgcgcagg
     7621 ccccctccag gtagaaggcc atttttccac cctgtagggg aagccgatta ttttgaatac
     7681 caccaagaag gtggcccaga tggtgagcct gacgtgcccc cgggagcgat agagcagggc
     7741 cccgcagatg acccaggaga aggcccaagc actggacccc ggggtcaggg tgatggaggc
     7801 aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc aaggaggttc caacccgaaa
     7861 tttgagaaca ttgcagaagg tttaagagct ctcctggcta ggagtcacgt agaaaggact
     7921 accgacgaag gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa gacctccctt
     7981 tacaacctaa ggcgaggaac tgcccttgct attccacaat gtcgtcttac accattgagt
     8041 cgtctcccct ttggaatggc ccctggaccc ggcccacaac ctggcccgct aagggagtcc
     8101 attgtctgtt atttcatggt ctttttacaa actcatatat ttgctgaggt tttgaaggat
     8161 gcgattaagg accttgttat gacaaagccc gctcctacct gcaatatcag ggtgactgtg
     8221 tgcagctttg acgatggagt agatttgcct ccctggtttc cacctatggt ggaaggggct
     8281 gccgcggagg gtgatgacgg agatgacgga gatgaaggag gtgatggaga tgagggtgag
     8341 gaagggcagg agtgatgtaa cttgttagga gacgccctca atcgtattaa aagccgtgta
     8401 ttcccccgca ctaaagaata aatccccagt agacatcatg cgtgctgttg gtgtatttct
     8461 ggccatctgt cttgtcacca ttttcgtcct cccaacatgg ggcaattgcc ggaaccctta
     8521 atataacttc gtataatgta tgctatacga agttattagg tccctcgaag aggttcacta
     8581 gcggatctca attgggcata cccatgttgt cacgtcactc agctccgcgc tcaacacctt
     8641 ctcgcgttgg aaaacattag cgacatttac ctggtgagca atcagacatg cgacggcttt
     8701 agcctggcct ccttaaattc acctaagaat gggagcaacc agcaggaaaa ggacaagcag
     8761 cgaaaattca cgcccccttg ggaggtggcg gcatatgcaa aggatagcac tcccactcta
     8821 ctactgggta tcatatgctg actgtatatg catgaggata gcatatgcta cccggataca
     8881 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     8941 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata
     9001 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     9061 gattaggata gcatatgcta cccagatata gattaggata gcatatgcta tccagatatt
     9121 tgggtagtat atgctaccca gatataaatt aggatagcat atactaccct aatctctatt
     9181 aggatagcat atgctacccg gatacagatt aggatagcat atactaccca gatatagatt
     9241 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatataaatt
     9301 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     9361 aggatagcct atgctaccca gatatagatt aggatagcat atgctatcca gatatttggg
     9421 tagtatatgc tacccatggc aacattagcc caccgtgctc tcagcgacct cgtgaatatg
     9481 aggaccaaca accctgtgct tggcgctcag gcgcaagtgt gtgtaatttg tcctccagat
     9541 cgcagcaatc gcgcccctat cttggcccgc ccacctactt atgcaggtat tccccggggt
     9601 gccattagtg gttttgtggg caagtggttt gaccgcagtg gttagcgggg ttacaatcag
     9661 ccaagttatt acacccttat tttacagtcc aaaaccgcag ggcggcgtgt gggggctgac
     9721 gcgtgccccc actccacaat ttcaaaaaaa agagtggcca cttgtctttg tttatgggcc
     9781 ccattggcgt ggagccccgt ttaattttcg ggggtgttag agacaaccag tggagtccgc
     9841 tgctgtcggc gtccactctc tttccccttg ttacaaatag agtgtaacaa catggttcac
     9901 ctgtcttggt ccctgcctgg gacacatctt aataacccca gtatcatatt gcactaggat
     9961 tatgtgttgc ccatagccat aaattcgtgt gagatggaca tccagtcttt acggcttgtc
    10021 cccaccccat ggatttctat tgttaaagat attcagaatg tttcattcct acactagtat
    10081 ttattgccca aggggtttgt gagggttata ttggtgtcat agcacaatgc caccactgaa
    10141 ccccccgtcc aaattttatt ctgggggcgt cacctgaaac cttgttttcg agcacctcac
    10201 atacacctta ctgttcacaa ctcagcagtt attctattag ctaaacgaag gagaatgaag
    10261 aagcaggcga agattcagga gagttcactg cccgctcctt gatcttcagc cactgccctt
    10321 gtgactaaaa tggttcacta ccctcgtgga atcctgaccc catgtaaata aaaccgtgac
    10381 agctcatggg gtgggagata tcgctgttcc ttaggaccct tttactaacc ctaattcgat
    10441 agcatatgct tcccgttggg taacatatgc tattgaatta gggttagtct ggatagtata
    10501 tactactacc cgggaagcat atgctacccg tttagggtta acaagggggc cttataaaca
    10561 ctattgctaa tgccctcttg agggtccgct tatcggtagc tacacaggcc cctctgattg
    10621 acgttggtgt agcctcccgt agtcttcctg ggcccctggg aggtacatgt cccccagcat
    10681 tggtgtaaga gcttcagcca agagttacac ataaaggcaa tgttgtgttg cagtccacag
    10741 actgcaaagt ctgctccagg atgaaagcca ctcagtgttg gcaaatgtgc acatccattt
    10801 ataaggatgt caactacagt cagagaaccc ctttgtgttt ggtccccccc cgtgtcacat
    10861 gtggaacagg gcccagttgg caagttgtac caaccaactg aagggattac at
//
