LOCUS       sequence-194751-        5227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Wnt5a in mammalian cells, zebrafish or Xenopus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pCS2+ mWnt5a
FEATURES             Location/Qualifiers
     source          1..5227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1276..1410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1300..1319)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1354..1373
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1523..1543)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1523..1541)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1562..1578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1562..1578)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1575..1597)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1586..1602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1610..1616,1617..1634,1635..1640))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1655..1676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1793..1810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1964..2552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2044..2063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2723..3514,3515..3583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3346..3365
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3584..3688)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3714..4169)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3851..3872)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4063..4082
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4243..5227
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccggccgg cctaccatga agaagcccat tggaatatta
      121 agcccgggag tggctttggg gaccgctgga ggtgccatgt cttccaagtt cttcctaatg
      181 gctttggcca cgtttttctc cttcgcccag gttgttatag aagctaattc ttggtggtct
      241 ctaggtatga ataaccctgt tcagatgtca gaagtatata tcataggtgc acagcctctc
      301 tgcagccaac tggcaggact ttctcaagga cagaagaaac tctgccactt gtatcaggac
      361 cacatgcagt acattggaga aggtgcgaag acaggcatca aggaatgcca gtaccagttc
      421 cggcatcgga gatggaactg cagcacagtg gacaatactt ctgtctttgg cagggtgatg
      481 caaataggca gccgagagac ggccttcacg tacgcggtga gcgcagctgg ggtggtgaac
      541 gccatgagcc gagcatgccg ggagggcgag ctgtctacct gtggctgcag ccgcgctgcg
      601 cgccccaagg acctgcctcg ggactggttg tggggcggct gcggagacaa catcgactat
      661 ggctaccgct tcgccaagga gttcgtggac gctagagaaa gggaacgaat ccacgctaag
      721 ggttcctatg agagcgcacg catcctcatg aacttacaca acaatgaagc aggccgtagg
      781 acagtataca acctggcaga tgtagcctgt aagtgtcatg gagtgtctgg ctcctgtagc
      841 ctcaagacgt gctggctgca gctggcggac ttccggaagg tgggcgatgc cctcaaggag
      901 aagtatgata gcgcggcggc catgaggctc aacagccggg gcaagctggt gcaggtcaac
      961 agccgcttca actccccgac cacgcaggac ctggtctaca tcgaccccag tccggactac
     1021 tgtgtgcgca acgagagcac tggctcgctg ggcacgcagg gacgcctgtg caacaagacc
     1081 tcagagggga tggacggctg cgagctcatg tgctgtgggc gtggctatga ccagtttaag
     1141 acagtgcaga ccgaacgctg tcattgcaag tttcactggt gctgctatgt caaatgcaag
     1201 aagtgcacgg agattgtgga tcagttcgtg tgcaaatagg cgcgcctcta gaactatagt
     1261 gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     1321 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1381 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     1441 caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg
     1501 gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     1561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1621 ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2401 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     3721 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3781 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3841 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3901 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3961 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     4021 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     4081 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     4141 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     4201 ggaagggcga tcggtgcggg cctcttcgct attacgccag tcgaccatag ccaattcaat
     4261 atggcgtata tggactcatg ccaattcaat atggtggatc tggacctgtg ccaattcaat
     4321 atggcgtata tggactcgtg ccaattcaat atggtggatc tggaccccag ccaattcaat
     4381 atggcggact tggcaccatg ccaattcaat atggcggact tggcactgtg ccaactgggg
     4441 aggggtctac ttggcacggt gccaagtttg aggaggggtc ttggccctgt gccaagtccg
     4501 ccatattgaa ttggcatggt gccaataatg gcggccatat tggctatatg ccaggatcaa
     4561 tatataggca atatccaata tggccctatg ccaatatggc tattggccag gttcaatact
     4621 atgtattggc cctatgccat atagtattcc atatatgggt tttcctattg acgtagatag
     4681 cccctcccaa tgggcggtcc catataccat atatggggct tcctaatacc gcccatagcc
     4741 actcccccat tgacgtcaat ggtctctata tatggtcttt cctattgacg tcatatgggc
     4801 ggtcctattg acgtatatgg cgcctccccc attgacgtca attacggtaa atggcccgcc
     4861 tggctcaatg cccattgacg tcaataggac cacccaccat tgacgtcaat gggatggctc
     4921 attgcccatt catatccgtt ctcacgcccc ctattgacgt caatgacggt aaatggccca
     4981 cttggcagta catcaatatc tattaatagt aacttggcaa gtacattact attggaagga
     5041 cgccagggta cattggcagt actcccattg acgtcaatgg cggtaaatgg cccgcgatgg
     5101 ctgccaagta catccccatt gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc
     5161 attgacgtaa atgggcggta ggcgtgccta atgggaggtc tatataagca atgctcgttt
     5221 agggaac
//
