LOCUS       sequence-194763-       10656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd1
FEATURES             Location/Qualifiers
     source          1..10656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3341..3475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3565..3589)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3597..3638
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3689..4277
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3742..3762)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4160..4171)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4349..4582
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4660..4781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4821..4956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4977..4995)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5005..5022)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5005..5021)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5014..5036)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5163..5618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5250..5269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5460..5481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5644..5748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5749..5817,5818..6609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5967..5986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6780..7368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7269..7288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7522..7539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7656..7677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7692..7697,7698..7715,7716..7722)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7730..7746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7735..7757
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7754..7770
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7754..7770
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7789..7809
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7791..7809
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7837..8063
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8064..8244
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8291..8416
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8909..9142
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9327..9371
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9602..9812
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9624..9820
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9671..9806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9852..10454
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9852..9871)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10348..10368
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10497..10614
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggct
     1381 gaggaggcgg cgcctagcga gtcccgggcc gccggccggc tgagcttgga actttgtgcc
     1441 gaagcactcc cgggccggcg ggaggaggtg gggcacgagg acacggccag ccaccgccgc
     1501 ccccgggctg atccccggcg ttgggctagc gggctgctgc tgctgctttg gttgctggag
     1561 gctcctctgc ttttgggggt ccgagcgcag gcggcgggcc aggtatccgg gccgggccag
     1621 caagccccgc cgccgcccca gccccagcag agcgggcagc agtacaacgg cgaacggggc
     1681 atctccatcc cggaccacgg ctactgccag cccatctcca tcccgctgtg cacggacatc
     1741 gcgtacaacc agaccatcat gcccaacctg ctgggccaca cgaatcagga ggacgccggt
     1801 ctggaggtgc accagttcta ccctctggtg aaggtgcagt gctccgccga gctcaagttc
     1861 ttcctgtgct ccatgtacgc gcctgtgtgc accgtactgg agcaggcgct accgccctgc
     1921 cgctccctgt gcgagcgcgc acgccagggc tgcgaggcgc tcatgaacaa gttcggcttc
     1981 cagtggccag acacactcaa gtgcgagaag ttcccggtgc acggcgcagg agagctgtgc
     2041 gtgggccaga acacgtccga caaaggcacc ccaactccct ccttgctacc agagttctgg
     2101 accagtaatc cgcagcacgg cggcggtggt taccgcggcg gctacccggg gggtgccggg
     2161 acggtggagc ggggaaagtt ctcctgcccg cgcgccctca gggtgccctc ctacctcaac
     2221 taccactttc tgggggagaa ggactgcggc gcaccctgcg aacccaccaa ggtttacggg
     2281 ctcatgtact tcgggccaga ggagctgcgc ttctcgcgca cctggatagg catctggtcc
     2341 gtgctgtgct gcgcctccac gctcttcacg gtgctcacgt acctagtgga catgcggcgc
     2401 ttcagctacc cggaacggcc catcattttc ctgtccggct gttacacagc ggtggcggtg
     2461 gcctacatcg ctggctttct gttggaggac cgggtggtgt gcaacgacaa gtttgcagag
     2521 gacggggcgc gcacggtggc gcagggcact aagaaagaag gctgcactat actctttatg
     2581 atgctctact tcttcagcat ggccagctcc atctggtggg tgatcctgtc cctcacctgg
     2641 ttcctggcag ccggcatgaa gtggggccac gaagccatcg aggccaactc acagtatttc
     2701 catttagccg cctgggctgt gccagccatc aaaactataa ccatcttggc gttgggccag
     2761 gtggatggcg acgtactgag cggagtgtgt tttgtggggc tcaacaacgt ggacgcactg
     2821 cgtggctttg tgctggcgcc tctcttcgtt tatctgttca ttggcacttc tttcctgctg
     2881 gccggtttcg tgtcactctt ccgcatccgc accatcatga agcatgacgg caccaagaca
     2941 gagaagctgg agaagctcat ggtgcgcatc ggagtcttca gtgtcctcta cactgtgccg
     3001 gccaccatcg tcatcgcctg ctacttctat gaacaggcct ttcgggacca gtgggagcgc
     3061 agctgggtgg cccagagctg caagagttat gccatccctt gccctcacct ccagggaggt
     3121 ggaggagtcc caccacaccc gcccatgagc ccagacttta cagtcttcat gatcaagtat
     3181 ctcatgacgc tgattgtggg catcacatcg ggcttctgga tctggtccgg caagacactg
     3241 aattcctgga ggaagttcta cacgaggctt accaacagca aacaggggga gactaccgtc
     3301 tgaggcgcgc ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata
     3361 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3421 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3481 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
     3541 cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtt gatatcggta
     3601 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact agtaccggtt
     3661 aagtcgacaa tcaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     3721 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3781 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3841 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3901 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3961 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4021 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4081 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4141 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4201 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4261 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     4321 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4381 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4441 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4501 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4561 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     4621 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     4681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     4981 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     5041 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     5101 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     5161 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5581 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     5641 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5701 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5761 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5821 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5881 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5941 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6001 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6061 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6121 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6181 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6241 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6301 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6361 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6421 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6481 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6541 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6601 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6661 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6721 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6781 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6841 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6901 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6961 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7021 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7081 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7141 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7201 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7261 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7321 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7381 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7441 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7501 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7561 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7621 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7681 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7741 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     7801 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     7861 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     7921 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     7981 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     8041 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     8101 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8161 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8221 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     8281 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     8341 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     8401 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     8461 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8521 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8581 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8641 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8701 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     8761 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     8821 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     8881 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     8941 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9001 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     9061 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     9121 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     9181 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     9241 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     9301 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     9361 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     9421 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     9481 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     9541 gacccatgca ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9601 agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct
     9661 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9721 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9781 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac
     9841 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     9901 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     9961 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
    10021 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga
    10081 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
    10141 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
    10201 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
    10261 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
    10321 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
    10381 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
    10441 ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
    10501 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
    10561 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
    10621 tattacaggg acagcagaga tccactttgg cgccgg
//
