LOCUS       sequence-194766-       10728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd3
FEATURES             Location/Qualifiers
     source          1..10728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3413..3547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3637..3661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3669..3710
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3761..4349
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3814..3834)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4232..4243)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4421..4654
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4732..4853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4893..5028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5049..5067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5077..5094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5077..5093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5086..5108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5235..5690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5322..5341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5532..5553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5716..5820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5821..5889,5890..6681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6039..6058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6852..7440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7341..7360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7594..7611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7728..7749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7764..7769,7770..7787,7788..7794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7802..7818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7807..7829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7826..7842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7826..7842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7861..7881
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7863..7881
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7909..8135
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8136..8316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8363..8488
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8981..9214
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9399..9443
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9674..9884
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9696..9892
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9743..9878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9924..10526
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9924..9943)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10420..10440
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10569..10686
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggct
     1381 gtgagctgga ttgtctttga tctttggctc ttgactgtgt ttctggggca gataggtggg
     1441 cacagtttgt tttcttgtga acctataacc ttgaggatgt gccaagattt gccttacaat
     1501 actaccttca tgcctaatct tctgaaccat tatgaccaac agactgcagc tttagcaatg
     1561 gagcccttcc accctatggt gaacctggat tgttctcggg attttcggcc atttctttgt
     1621 gcactctatg cccctatttg tatggaatat ggacgtgtca cacttccctg ccgtaggctg
     1681 tgtcagcgtg cctatagcga gtgttcaaaa ctcatggaga tgtttggtgt cccgtggcct
     1741 gaagatatgg agtgcagtag gtttccagat tgtgatgagc catatccccg acttgtggat
     1801 ttgaatttag ttggagatcc aactgaagga gccccagttg cagtgcagag ggactatggt
     1861 ttttggtgtc ccagagagtt aaaaattgat cctgatcttg gctattcctt tctgcacgtg
     1921 cgagattgtt cgccaccatg tcccaatatg tacttcagga gagaagaact gtcatttgct
     1981 cgctatttca taggcctgat ttcaatcatt tgcctctctg ccacattgtt tactttttta
     2041 acctttctaa ttgacgtcac aagattccgt taccctgaaa gacctatcat attttatgca
     2101 gtctgctaca tgatggtgtc attaattttc ttcattgggt ttttgctgga ggaccgagta
     2161 gcctgcaatg catctagccc tgcacagtat aaggcttcta cagtgacaca aggatctcac
     2221 aataaggcct gtaccatgct ctttatggta ctatattttt tcactatggc tggcagtgta
     2281 tggtgggtaa ttcttaccat cacatggttt ttagcagctg tgccaaagtg gggcagtgaa
     2341 gctattgaga agaaagcatt gctgtttcat gccagtgcct ggggcatccc cggaactcta
     2401 actatcatcc ttttagcgat gaataaaatt gaaggtgaca atattagtgg cgtgtgtttt
     2461 gtcggcctct acgacgttga tgcattaaga tatttcgttc tcgctcccct ctgcctgtat
     2521 gtggtagttg gggtttctct ccttttagcc ggcattatat ccctaaacag agttcggatt
     2581 gagatcccat tagaaaagga aaaccaagat aagttagtga agttcatgat ccggattggt
     2641 gttttcagca ttctctacct tgtgccactc ttggttgtaa ttggatgtta cttttatgag
     2701 caagcttacc gcggcatctg ggagacaaca tggatccagg aacgctgcag agagtatcac
     2761 attccatgtc cgtaccaggt tactcagatg agtcgtccag acctgattct ctttctgatg
     2821 aagtatctca tggctctcat agttgggatt ccctctatat tttgggttgg aagcaaaaag
     2881 acatgctttg aatgggccag ttttttccat gggcgtagga aaaaagagat agtgaatgag
     2941 agccggcagg tgctccagga acctgacttt gctcagtcac tcctgaggga cccaaatact
     3001 ccaattataa gaaaatcaag aggaacttcc actcaaggga catccacaca tgcttcttca
     3061 actcagctgg ccatggtgga tgaccaaaga agcaaagcag ggagtgtcca cagcaaagtg
     3121 agcagctacc atggcagcct ccacaggtca cgggatggca ggtacactcc ctgcagttac
     3181 cgaggaatgg aggagagact acctcacggc agcatgtcac ggctgacgga tcattccagg
     3241 cacagtagtt ctcatcggct caacgagcag tcccgacaca gcagcatccg agacctcagt
     3301 aacaacccca tgactcacat tacacatggc accagcatga accgtgttat tgaggaggat
     3361 ggaaccagtg cttagggcgc gcctctagaa ctatagtgag tcgtattacg tagatccaga
     3421 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3481 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3541 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     3601 ggttttttaa ttcgcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg
     3661 ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa
     3721 ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc tggattacaa
     3781 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3841 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3901 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3961 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4021 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4081 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4141 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4201 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4261 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4321 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     4381 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4441 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4501 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4561 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4621 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     4681 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     4741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4861 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4921 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4981 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     5041 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     5101 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5161 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5221 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5281 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5341 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5401 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5461 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5521 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5581 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5641 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     5701 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5761 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5821 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5881 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5941 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6001 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6061 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6121 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6181 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6241 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6301 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6361 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6421 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6481 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6541 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6601 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6661 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6721 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6781 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6841 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6901 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6961 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7021 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7081 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7141 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7201 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7261 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7321 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7381 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7441 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7501 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7561 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7621 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7681 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7741 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7801 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     7861 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     7921 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     7981 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     8041 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     8101 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     8161 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8221 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8281 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     8341 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     8401 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     8461 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     8521 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     8581 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     8641 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     8701 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8761 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8821 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8881 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8941 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     9001 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     9061 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     9121 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     9181 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     9241 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     9301 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     9361 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     9421 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     9481 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     9541 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     9601 caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc
     9661 cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat
     9721 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9841 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc
     9901 tagaggtacc accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     9961 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
    10021 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
    10081 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg
    10141 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
    10201 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
    10261 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
    10321 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
    10381 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
    10441 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
    10501 tgacccgcaa gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc
    10561 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10621 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10681 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccgg
//
