LOCUS       sequence-194768-       10341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd4
FEATURES             Location/Qualifiers
     source          1..10341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3026..3160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3250..3274)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3282..3323
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3374..3962
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3427..3447)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3845..3856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4034..4267
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4345..4466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4506..4641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4662..4680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4690..4707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4690..4706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4699..4721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4848..5303
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4935..4954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5145..5166
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5329..5433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5434..5502,5503..6294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5652..5671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6465..7053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6954..6973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7207..7224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7341..7362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7377..7382,7383..7400,7401..7407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7415..7431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7420..7442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7439..7455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7439..7455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7474..7494
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7476..7494
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7522..7748
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7749..7929
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7976..8101
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8594..8827
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9012..9056
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9287..9497
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9309..9505
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9356..9491
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9537..10139
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9537..9556)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10033..10053
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10182..10299
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggcc
     1381 tggccgggca cagggccgag cagccggggg gcgcctggag gcgtcgggct caggctgggg
     1441 ctgctgctgc agttcctcct gctcctgcgg ccgacactgg ggttcgggga cgaggaggag
     1501 cggcgctgcg accccatccg catcgccatg tgccagaacc tcggctacaa cgtgaccaag
     1561 atgcccaact tagtgggaca cgagctgcag acagacgccg agctgcagct gacaactttc
     1621 acgccgctca tccagtacgg ctgctccagc cagctgcagt tcttcctttg ttcggtttat
     1681 gtgccaatgt gcacagagaa gatcaacatc cccatcggcc cgtgcggtgg catgtgcctt
     1741 tcagtcaaga gacgctgtga accagtcctg agagaatttg ggtttgcctg gcccgacacc
     1801 ctgaactgca gcaagttccc gccccagaac gaccacaacc acatgtgcat ggaaggacca
     1861 ggtgatgaag aggttccctt gccccacaag actcccatcc agcccgggga agagtgccac
     1921 tccgtgggaa gcaattctga tcagtacatc tgggtgaaga ggagcctgaa ctgtgttctc
     1981 aagtgtggct acgatgctgg cttgtacagc cgctcagcta aggagttcac ggatatttgg
     2041 atggctgtgt gggccagcct ctgcttcatc tccaccacct tcaccgtgct gaccttcctg
     2101 attgattcat ccaggttttc ttaccctgag cgccccatca tatttctcag tatgtgctat
     2161 aatatttata gcattgctta tattgttcgg ctgactgtag gccgggaaag gatatcctgt
     2221 gattttgaag aggcggcaga gcccgttctc atccaagaag gacttaagaa cacaggatgt
     2281 gcaataattt tcttgctgat gtactttttt ggaatggcca gctccatttg gtgggttatt
     2341 ctgacactca cttggttttt ggcagccgga ctcaagtggg gtcatgaagc cattgaaatg
     2401 cacagttctt atttccacat cgcagcctgg gctattcccg cagtgaaaac cattgtcatc
     2461 ttgattatga gactagtgga tgccgatgaa ctgactggct tgtgctatgt tgggaaccaa
     2521 aacctagatg ccctcactgg ctttgtggtg gctcctctct ttacgtattt ggtgattgga
     2581 acgctgttca ttgcggcggg tttggtggcc ttattcaaaa ttcggtccaa tcttcaaaaa
     2641 gacgggacaa agacagacaa gttggaaagg ctaatggtca agatcggggt cttctcagta
     2701 ctgtacacgg ttcctgcaac ctgtgtgatt gcctgttatt tctatgaaat ctcaaactgg
     2761 gcactctttc gatattctgc agatgactca aacatggcag ttgaaatgtt gaaaattttt
     2821 atgtctttgc tcgtgggcat cacttcaggc atgtggattt ggtctgccaa aactcttcac
     2881 acgtggcaaa agtgttctaa ccgattggtg aattctggga aggtaaagag agagaagagg
     2941 gggaatggtt gggtgaagcc aggaaaaggc aacgagactg tggtataagg cgcgcctcta
     3001 gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg
     3061 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     3181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcactcga
     3241 gatatctaga cccagctttc ttgtacaaag tggttgatat cggtaagcct atccctaacc
     3301 ctctcctcgg tctcgattct acgtagtaat gaactagtac cggttaagtc gacaatcaac
     3361 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3421 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3481 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3541 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3601 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3661 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3721 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3781 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3841 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3901 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3961 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4021 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4081 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4141 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4201 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4261 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     4321 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     4381 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4441 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     4501 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4561 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4621 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     4681 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4741 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4801 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     4861 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4921 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4981 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5041 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5101 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5161 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5221 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5281 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     5341 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5401 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5461 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5521 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5581 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5641 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5701 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5761 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5821 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5881 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5941 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6001 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6061 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6121 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6181 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6241 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7261 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7321 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7381 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7441 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     7501 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     7561 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     7621 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     7681 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     7741 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7801 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7861 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7921 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     7981 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8041 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8101 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8161 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8221 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8281 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8341 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     8401 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     8461 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     8521 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     8581 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     8641 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     8701 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     8761 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     8821 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     8881 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     8941 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     9001 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     9061 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     9121 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     9181 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca
     9241 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9301 gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9361 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9421 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9481 ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga
     9541 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     9601 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     9661 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     9721 gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg
     9781 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     9841 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     9901 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     9961 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
    10021 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
    10081 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac
    10141 gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
    10201 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
    10261 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
    10321 agagatccac tttggcgccg g
//
