LOCUS       sequence-194778-       10485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd5
FEATURES             Location/Qualifiers
     source          1..10485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3170..3304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3394..3418)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3426..3467
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3518..4106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3571..3591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3989..4000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4178..4411
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4489..4610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4650..4785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4806..4824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4834..4851)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4834..4850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4843..4865)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4992..5447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5079..5098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5289..5310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5473..5577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5578..5646,5647..6438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5796..5815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6609..7197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7098..7117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7351..7368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7485..7506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7521..7526,7527..7544,7545..7551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7559..7575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7564..7586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7583..7599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7583..7599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7618..7638
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7620..7638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7666..7892
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7893..8073
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8120..8245
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8738..8971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9156..9200
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9431..9641
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9453..9649
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9500..9635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9681..10283
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9681..9700)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10177..10197
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10326..10443
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggct
     1381 cgacccgacc cgtctgcgcc tccctctctt ctgctgctgt tgctggcgca gctggtgggc
     1441 cgggcagcgg ccgcctccaa ggccccggtg tgccaggaaa tcacggtgcc catgtgccga
     1501 ggcatcggct acaacctgac gcacatgccc aaccagttca accatgacac gcaggacgaa
     1561 gcaggcctgg aggtgcacca attctggccg cttgtggaga tccactgctc accggacctg
     1621 cgcttcttcc tgtgctctat gtacacgccc atctgtttgc ctgactacca caagccgcta
     1681 ccaccgtgcc gttccgtgtg cgagcgcgcc aaggccggct gctcgccgct catgcgccag
     1741 tacggcttcg cctggcccga gcgcatgagc tgcgaccgcc tccctgtgct gggcggcgac
     1801 gccgaggttc tgtgtatgga ttataaccga agcgaagcca ccaccgcgtc ccctaagtcc
     1861 ttcccggcca aacctacact cccaggacca ccaggggcgc catcttccgg gggcgagtgc
     1921 ccctcgggag gcccatccgt gtgcacgtgc cgcgagccct tcgtgcccat cctgaaggag
     1981 tcacacccac tctacaacaa ggtgcgcacc ggccaagtgc ccaactgcgc ggtgccctgc
     2041 taccagccgt ccttcagccc ggacgagcgc acgttcgcca ccttctggat tggcctgtgg
     2101 tctgtgctgt gcttcatctc cacgtccacc accgttgcca ccttcctcat tgacatggaa
     2161 cgattccgct accctgagcg ccccatcatc ttcttgtctg cgtgctacct gtgtgtgtca
     2221 ctgggattct tggtgcgcct ggtagtgggc catgccagcg tcgcttgcag ccgtgagcac
     2281 agccacattc actatgagac taccggccct gcgctgtgca cggttgtctt cctcttagtc
     2341 tatttctttg gcatggccag ctccatctgg tgggtcatcc tgtcgctcac ctggttcttg
     2401 gcggctggca tgaagtgggg caatgaagcc atcgcaggtt atgcacagta cttccacctt
     2461 gctgcctggc tcatccccag tgtcaagtcc attacggcgc tggcactgag ctcggtggac
     2521 ggggacccag tggctggcat ctgctatgtg ggcaaccaaa acctgaactc actacgaggc
     2581 tttgtcttgg gcccactggt gctgtacctg ttggtgggca cgctcttcct tctggcaggc
     2641 ttcgtgtcac tcttccgcat ccggagcgtc atcaagcagg gtggcactaa gacggacaag
     2701 ctagagaagc tcatgatccg catcggcatc ttcaccctgc tctacacggt gccagccagc
     2761 atcgtggtgg cctgctacct gtatgagcag cactaccggg agagctggga ggcagccctc
     2821 acctgcgcgt gtccgggacc ggacgctggc cagccacgcg ccaaacccga gtactgggtg
     2881 ctcatgctca agtacttcat gtgcctggtg gtgggcatca cgtcgggagt ctggatctgg
     2941 tccggcaaga ctctggagtc ttggcggcgg ttcaccagcc gctgctgctg cagctctcgg
     3001 cggggccaca agagcggtgg cgctatggcc gcaggagact atgcggaggc cagcgccgcg
     3061 ctcaccggca ggaccgggcc gcctggcccc accgccgcat accacaagca agtgtccctg
     3121 tcgcacgtat agggcgcgcc tctagaacta tagtgagtcg tattacgtag atccagacat
     3181 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3241 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3301 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3361 tttttaattc gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttg
     3421 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta
     3481 gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg attacaaaat
     3541 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3601 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3661 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3721 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3781 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3841 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3901 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     3961 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4021 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4081 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     4141 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4201 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4381 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     4441 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     4501 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4561 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     4621 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4681 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4741 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     4801 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     4861 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4921 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4981 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5401 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     5461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5521 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5581 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5641 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5701 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5761 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5821 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5881 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5941 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6001 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6061 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6121 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6181 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6241 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6301 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6361 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6421 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6481 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6601 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6661 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6721 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6781 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6841 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6901 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6961 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7021 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7081 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7141 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7201 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7261 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7321 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7381 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7441 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7501 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7561 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     7621 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     7681 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     7741 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     7801 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     7861 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     7921 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7981 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8041 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     8101 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8161 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8221 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8281 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8341 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8401 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8461 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8521 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8581 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8641 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8701 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8761 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8821 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8881 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8941 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     9001 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     9061 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     9121 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     9181 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     9241 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     9301 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     9361 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9421 aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt
     9481 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9541 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9601 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag
     9661 aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     9721 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     9781 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     9841 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg
     9901 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     9961 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
    10021 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
    10081 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
    10141 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
    10201 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
    10261 cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc
    10321 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
    10381 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
    10441 tttcgggttt attacaggga cagcagagat ccactttggc gccgg
//
