LOCUS       sequence-194782-       10446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd7
FEATURES             Location/Qualifiers
     source          1..10446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3131..3265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3355..3379)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3387..3428
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3479..4067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3532..3552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3950..3961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4139..4372
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4450..4571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4611..4746
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4767..4785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4795..4812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4795..4811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4804..4826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4953..5408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5040..5059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5250..5271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5434..5538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5539..5607,5608..6399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5757..5776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6570..7158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7059..7078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7312..7329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7446..7467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7482..7487,7488..7505,7506..7512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7520..7536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7525..7547
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7544..7560
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7544..7560
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7579..7599
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7581..7599
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7627..7853
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7854..8034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8081..8206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8699..8932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9117..9161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9392..9602
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9414..9610
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9461..9596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9642..10244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9642..9661)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10138..10158
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10287..10404
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatgcgg
     1381 ggccccggca cggcggcgtc gcactcgccc ctgggcctct gcgccctggt gcttgctctt
     1441 ctgggcgcgc tgcccacgga cacccgggct cagccatatc acggcgagaa aggcatctcg
     1501 gtaccggacc acggcttctg ccagcccatc tccatcccgt tgtgcacgga tatcgcctac
     1561 aaccagacca tcctgcccaa cctgctgggc cacacgaacc aagaggacgc gggcctcgag
     1621 gtgcaccagt tctaccctct ggtaaaggtg cagtgttctc ctgagctacg cttcttctta
     1681 tgctctatgt acgcacccgt gtgcaccgtg ctcgaccaag ccattcctcc gtgccgttcc
     1741 ttgtgcgagc gcgcccgaca gggctgcgag gcgctcatga acaagttcgg cttccagtgg
     1801 ccagagcggt tgcgctgcga gaacttccca gtgcacggtg ccggcgagat ctgcgtgggg
     1861 cagaacacgt ccgacggctc cgggggcgcg ggcggcagtc ccaccgccta ccctactgct
     1921 ccctacctgc cagacccacc tttcactgcg atgtccccct cagatggcag aggccgcttg
     1981 tctttcccct tctcgtgtcc gcgccagctc aaggtgcccc cctacctggg ctaccgcttc
     2041 ctaggtgagc gtgactgcgg tgccccgtgt gagccgggcc gtgctaacgg cctcatgtac
     2101 tttaaagaag aggagagacg gttcgcccgc ctctgggtgg gtgtgtggtc agtgctgtgc
     2161 tgcgcctcga cgctcttcac ggtgctcacc tacctagtgg acatgcgtcg cttcagctat
     2221 ccagagcgac ccatcatctt cctgtcgggt tgctacttca tggtggcagt ggcgcacgtg
     2281 gcaggcttcc tgctagagga ccgtgccgtg tgcgtggagc gcttctcgga cgatggctac
     2341 cgcacggtgg cgcagggcac caagaaggag ggctgcacca tcctcttcat ggtgctttac
     2401 ttcttcggta tggccagctc catctggtgg gtcattctgt ccctcacttg gttcctggca
     2461 gctggcatga agtggggcca cgaggccatc gaggccaact cgcagtactt tcatctggcc
     2521 gcgtgggctg tgccagcggt caagacaatc accattttgg ccatgggcca ggtggatggt
     2581 gacctactca gtggagtgtg ctacgtgggc ctgtctagtg tggatgcatt gcggggcttc
     2641 gtgctggcgc ccttgttcgt ctacctcttc atcgggacgt ccttcctgtt ggccggcttt
     2701 gtgtctctct ttcgcatccg caccatcatg aagcacgacg gcaccaagac agagaagctg
     2761 gagaagctga tggtgcgcat cggcgtcttc agcgtgctct acacggtgcc ggccaccatc
     2821 gtgttggcct gctactttta tgagcaggcc ttccgagagc actgggaacg cacctggctc
     2881 ctgcagactt gcaagagcta cgctgtgccc tgccctccgg gccacttctc tcccatgagc
     2941 cccgacttta cagtcttcat gatcaagtac ctgatgacca tgatcgtggg catcactacg
     3001 ggcttctgga tctggtcggg caagaccctg cagtcatggc gtcgcttcta ccacagactc
     3061 agccacagca gcaaggggga aactgcggta tgaggcgcgc ctctagaact atagtgagtc
     3121 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3181 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3241 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3301 tcagggggag gtgtgggagg ttttttaatt cgcggccgca ctcgagatat ctagacccag
     3361 ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg
     3421 attctacgta gtaatgaact agtaccggtt aagtcgacaa tcaacgcgtt aagtcgacaa
     3481 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3541 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3601 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3661 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3721 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3781 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3841 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3901 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3961 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4021 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     4081 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4141 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4201 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4261 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4321 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     4381 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4441 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4501 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4561 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4681 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4741 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4801 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4861 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4921 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4981 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5041 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5101 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5161 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5221 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5281 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5341 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5401 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5461 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5521 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5581 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5641 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5701 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5761 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5821 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5881 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5941 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6001 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6061 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6121 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6181 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6241 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6301 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6361 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6421 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6481 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6541 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6601 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6661 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6721 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6781 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6841 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6901 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6961 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7021 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7081 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7141 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7201 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7261 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7321 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7381 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7441 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7501 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7561 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7621 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7681 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7741 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7801 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7861 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7921 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7981 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8041 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8101 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8161 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8221 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8281 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8341 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8401 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8461 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8521 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9301 cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc
     9361 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct
     9421 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9481 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9541 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9601 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
     9661 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     9721 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     9781 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     9841 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     9901 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     9961 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    10021 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    10081 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    10141 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    10201 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
    10261 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    10321 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    10381 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
    10441 cgccgg
//
