LOCUS       sequence-194783-       10784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd8
FEATURES             Location/Qualifiers
     source          1..10784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3469..3603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3693..3717)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3725..3766
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3817..4405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3870..3890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4288..4299)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4477..4710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4788..4909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4949..5084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5105..5123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5133..5150)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5133..5149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5142..5164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5291..5746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5378..5397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5588..5609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5772..5876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5877..5945,5946..6737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6095..6114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6908..7496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7397..7416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7650..7667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7784..7805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7820..7825,7826..7843,7844..7850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7858..7874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7863..7885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7882..7898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7882..7898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7917..7937
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7919..7937
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7965..8191
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8192..8372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8419..8544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9037..9270
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9455..9499
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9730..9940
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9752..9948
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9799..9934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9980..10582
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9980..9999)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10476..10496
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10625..10742
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggag
     1381 tggggttacc tgttggaagt gacctcgctc ctagccgcct tggcggtgct acagcgctct
     1441 agcggcgctg ccgcggcttc ggccaaggag ctggcgtgcc aagagatcac ggtgccgttg
     1501 tgcaaaggca tcggttacaa ctacacttac atgcccaacc agttcaacca cgacacgcaa
     1561 gatgaggcgg gcctagaggt gcaccagttt tggccgctgg tggagataca gtgctccccg
     1621 gacctcaagt tctttctgtg tagcatgtac acgcccatct gcctggagga ctacaagaag
     1681 cctctgccgc cttgtcgctc tgtgtgtgaa cgcgccaagg ccggctgcgc gccgctcatg
     1741 cgccagtacg gctttgcttg gcctgaccgc atgcgctgcg atcggttgcc ggagcagggc
     1801 aacccggaca ctctgtgcat ggactacaac cgcaccgacc tcaccacggc cgcgcccagc
     1861 ccaccgcgcc gcctgcctcc gccgcctcct cccggcgagc agccgccctc tggcagcggc
     1921 cacagccgcc cgccaggggc caggccccca catcgtggcg gcagcagtag gggcagcggg
     1981 gacgcggcgg ctgcgccccc ttcgcgcggc gggaaggcga ggccccctgg tggcggcgct
     2041 gctccctgcg agccggggtg ccagtgccgc gcgcccatgg tgagcgtgtc cagcgaacgc
     2101 cacccgctct acaaccgcgt caagaccggc cagatcgcca actgtgcgct gccctgccac
     2161 aaccccttct ttagccagga tgagcgcgcc ttcaccgtct tctggatcgg cctgtggtcg
     2221 gtgctctgct tcgtctccac cttcgccact gtctctacct tcctcatcga tatggagcgc
     2281 tttaagtacc cggaacggcc catcatattc ctctccgcct gttacctctt cgtgtctgtc
     2341 gggtacctgg tgcgcctggt ggcaggacat gagaaagtgg cctgcagcgg cggcgctccg
     2401 ggtgctggcg gagctggggg tgcgggcggc gcggcggcgg ctggcgcagg ggcagcggga
     2461 gcgggggcga gcagcccggg cgcgcgcggc gagtacgagg agctgggcgc agttgagcag
     2521 catgttcgct atgagaccac tggccccgcg ctgtgcacgg tggtctttct ccttgtctac
     2581 ttttttggca tggccagctc catctggtgg gtaatcctgt cgctcacgtg gttcttggca
     2641 gctggcatga agtggggtaa cgaggccata gcaggctact cgcagtactt ccacctggcc
     2701 gcgtggcttg tgcccagcgt caagtccatc gcggtgctgg cgctcagctc cgtagacggc
     2761 gacccggtgg cgggcatctg ctacgtgggc aaccagagcc ttgacaacct acgcggcttt
     2821 gtgctggcgc cactggttat ctacctcttc attgggacta tgtttctgtt agctggcttc
     2881 gtgtcgctgt tccgaatccg ttcagtcatc aagcagcaag gaggtccaac taagacacac
     2941 aagctagaaa aactcatgat ccgcttgggc ctcttcaccg tgctctacac ggtgcccgct
     3001 gccgtcgttg tcgcctgcct tttctatgag cagcacaacc gaccgcgctg ggaggccacg
     3061 cacaactgcc catgccttcg ggacctgcaa ccggaccagg ctcgcaggcc cgattacgcg
     3121 gtcttcatgc tcaagtactt catgtgccta gtagtgggca tcacatcggg cgtgtgggtc
     3181 tggtccggca agactctgga gtcctggcgc gcgttgtgca ctaggtgctg ctgggccagc
     3241 aagggcgctg cagtaggcgc gggcgctgga ggcagcggcc ctgggggcag tggacccggg
     3301 cccggcggag gtgggggaca cggcggaggc gggggatccc tctacagcga cgtcagtacc
     3361 ggcctgacgt ggcggtctgg cacggccagc tctgtatctt accctaagca aatgccattg
     3421 tcccaggtct gagcgcgcct ctagaactat agtgagtcgt attacgtaga tccagacatg
     3481 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3541 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3601 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3661 ttttaattcg cggccgcact cgagatatct agacccagct ttcttgtaca aagtggttga
     3721 tatcggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag
     3781 taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     3841 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3901 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3961 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4021 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4081 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4141 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4201 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4261 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4321 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4381 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     4441 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4501 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4561 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4621 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4681 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     4741 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4921 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4981 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5041 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     5101 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     5161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     5221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5281 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5341 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5581 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5641 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5701 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5761 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5821 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5881 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5941 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6001 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6061 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6121 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6181 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6241 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6301 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6361 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6421 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6481 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6541 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6601 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6661 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6721 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6781 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7021 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7801 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7861 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7921 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7981 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     8041 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     8101 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     8161 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     8221 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8281 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8341 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8401 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8461 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8521 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8581 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8641 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8701 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8761 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8821 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8881 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8941 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     9001 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     9061 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     9121 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     9181 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     9241 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9301 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9361 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9421 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9481 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9541 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9601 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9661 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9721 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     9781 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9841 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9901 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     9961 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
    10021 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
    10081 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
    10141 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
    10201 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
    10261 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
    10321 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
    10381 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
    10441 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
    10501 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
    10561 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
    10621 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    10681 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    10741 ttcgggttta ttacagggac agcagagatc cactttggcg ccgg
//
