LOCUS       sequence-194784-       10505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd9
FEATURES             Location/Qualifiers
     source          1..10505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3190..3324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3414..3438)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3446..3487
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3538..4126
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3591..3611)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4009..4020)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4198..4431
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4509..4630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4670..4805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4826..4844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4854..4871)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4854..4870)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4863..4885)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5012..5467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5099..5118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5309..5330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5493..5597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5598..5666,5667..6458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5816..5835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6629..7217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7118..7137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7371..7388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7505..7526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7541..7546,7547..7564,7565..7571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7579..7595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7584..7606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7603..7619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7603..7619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7638..7658
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7640..7658
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7686..7912
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7913..8093
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8140..8265
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8758..8991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9176..9220
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9451..9661
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9473..9669
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9520..9655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9701..10303
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9701..9720)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10197..10217
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10346..10463
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggcc
     1381 gtgccaccgc tgctccgcgg ggcgctgctg ctgtggcagc tgctggcgac gggcggcgcg
     1441 gcgctggaga tcggccgctt cgatccggag cgtggccgtg gccccgcgcc gtgccaagcg
     1501 atggagatcc ccatgtgccg gggcattggc tacaacctga cccgcatgcc caacctactg
     1561 ggccacacgt cgcagggcga ggcggcggcg cagctggccg agttctcgcc cctcgtgcag
     1621 tacggctgcc acagccacct gcgcttcttc ctctgctcgc tctacgcccc aatgtgcacc
     1681 gaccaggtct ccactcccat cccagcctgc cggcccatgt gcgagcaggc tcgcctgcgc
     1741 tgcgccccca tcatggagca attcaatttc ggctggcccg actcactcga ctgtgcccgg
     1801 ctccccacgc gcaacgaccc gcacgcactc tgtatggagg cacccgagaa cgctacagca
     1861 ggccccacag aaccccacaa gggcctgggc atgctccctg tggcacctcg gcccgcgagg
     1921 ccaccgggag attcagcccc aggtcccggc agcggtggca cctgcgacaa ccccgagaag
     1981 ttccagtacg tggagaagag tcgctcgtgc gctccgcgct gcgggccagg cgtcgaggtg
     2041 ttctggtctc ggcgcgataa ggacttcgcg ctggtttgga tggctgtgtg gtccgcgttg
     2101 tgtttcttct ccacggcctt caccgtgttc accttcctgc tggagcctca ccggttccag
     2161 tacccagagc gcccgattat cttcctttct atgtgctaca atgtctactc cttggccttc
     2221 ctgatcagag cggtggcagg tgcacagagt gtggcatgcg accaggaggc aggggctctg
     2281 tatgtgatcc aggagggtct ggaaaacaca ggctgcaccc tggtcttcct gttgctctat
     2341 tatttcggga tggccagctc actttggtgg gtagttttga ctctaacctg gttcctggct
     2401 gcaggcaaaa aatggggcca cgaggccatc gaggctcacg gcagctactt ccacatggca
     2461 gcgtggggcc tgccagcact caaaactatc gtggtcctga ctctgcgcaa ggtggctggc
     2521 gatgaactga ctgggctctg ctatgtagcc agcatggacc cggcagccct cactggcttt
     2581 gtgttggtac ccctatcttg ctacctggta ctgggtacca gtttcctcct gaccggtttt
     2641 gtggctctct tccatatccg caaaatcatg aagacgggag gcaccaatac ggagaagctg
     2701 gagaagctga tggtcaaaat cggagtcttt tccatccttt acacagtgcc ggccacctgc
     2761 gtcattgtct gctacgttta tgaacgcctc aacatggact tctggcggct tcgggccaca
     2821 gagcaaccat gtactgctgc caccgtgcct ggaggccgga gagactgctc gctgccaggg
     2881 ggctcggtgc ccactgtggc tgtcttcatg ctcaaaatct tcatgtcctt ggtggtgggc
     2941 atcaccagtg gagtctgggt atggagttcc aagacttttc agacttggca gagcctgtgc
     3001 taccgaaaaa tggcagctgg ccgagcccgg gccaaggcct gccgaacccc agggggctat
     3061 ggccggggta cccactgcca ctacaaagcc cccacggtgg tcttgcacat gactaagaca
     3121 gacccctctc tggagaaccc cacacacctc taggcgcgcc tctagaacta tagtgagtcg
     3181 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3361 cagggggagg tgtgggaggt tttttaattc gcggccgcac tcgagatatc tagacccagc
     3421 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     3481 ttctacgtag taatgaacta gtaccggtta agtcgacaat caacgcgtta agtcgacaat
     3541 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3601 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3661 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3721 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3781 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3841 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3901 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3961 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4021 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4081 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     4141 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4201 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4261 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4321 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4381 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4441 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4501 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4561 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4621 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4681 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4741 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4801 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4861 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4921 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4981 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5041 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5101 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5161 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5221 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5281 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5341 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5401 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5461 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5521 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5581 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5641 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5701 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5761 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5821 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5881 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5941 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6001 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6061 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6121 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6181 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6241 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6301 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6361 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6421 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7321 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7381 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7441 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7501 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7561 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7621 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7681 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7741 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7801 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7861 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7921 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7981 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8041 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8101 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8161 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8221 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8281 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8341 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8401 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8461 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8521 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8581 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8641 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8701 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8761 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8821 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8881 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8941 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9001 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9061 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9121 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9181 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9241 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9301 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9361 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     9421 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     9481 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9541 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9601 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9661 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9721 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9781 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9841 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9901 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9961 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
    10021 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
    10081 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
    10141 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
    10201 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    10261 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
    10321 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10381 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10441 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10501 gccgg
//
