LOCUS       sequence-194892-        9541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis
            in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor 
            E, Welker E
  TITLE     Crossing enhanced and high fidelity SpCas9 nucleases to optimize 
            specificity and cleavage.
  JOURNAL   Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
  PUBMED    28985763
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2017-10-6"; volume: "18(1):190. doi"; pages: " 
            10.1186/s13059-017-1318-8"
COMMENT     Sequence Label: pET-dSpCas9-HF1-VP64-6xHis
FEATURES             Location/Qualifiers
     source          1..9541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4198..4218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4225..4290
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4321..4470
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4471..4488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4507..4551
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4589..4607)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4603..4650
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4687..5142
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4774..4793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4984..5005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5257..6048,6049..6117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5880..5899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6118..6210)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6291..6879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6780..6799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7033..7050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7065..7204
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7130..7149)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7290..7312
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7306..7497)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8069..9151)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9113..9132
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9152..9229)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9435..9454
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg gataagaaat actcaatagg cttagctatc
      121 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
      181 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
      241 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
      301 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
      361 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
      421 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
      481 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
      541 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
      601 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
      661 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
      721 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
      781 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
      841 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
      901 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
      961 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
     1021 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
     1081 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
     1141 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaggaagag
     1201 ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg
     1261 aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc
     1321 caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca
     1381 ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac
     1441 gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgacaagaaa gtccgaagaa
     1501 acgattactc cctggaattt tgaggaagtt gtcgataaag gtgcgtcagc tcaatcgttc
     1561 atcgagagga tgaccgcctt tgacaagaat ttaccgaacg aaaaagtatt gcctaagcac
     1621 agtttacttt acgagtattt cacagtgtac aatgaactca cgaaagttaa gtatgtcact
     1681 gagggcatgc gtaaacccgc ctttctaagc ggagaacaga agaaagcaat agtagatctg
     1741 ttattcaaga ccaaccgcaa agtgacagtt aagcaattga aagaggacta ctttaagaaa
     1801 attgaatgct tcgattctgt cgagatctcc ggggtagaag atcgatttaa tgcgtcactt
     1861 ggtacgtatc atgacctcct aaagataatt aaagataagg acttcctgga taacgaagag
     1921 aatgaagata tcttagaaga tatagtgttg actcttaccc tctttgaaga tcgggaaatg
     1981 attgaggaaa gactaaaaac atacgctcac ctgttcgacg ataaggttat gaaacagtta
     2041 aagaggcgtc gctatacggg ctggggagcc ttgtcgcgga aacttatcaa cgggataaga
     2101 gacaagcaaa gtggtaaaac tattctcgat tttctaaaga gcgacggctt cgccaatagg
     2161 aactttatgg ccctgatcca tgatgactct ttaaccttca aagaggatat acaaaaggca
     2221 caggtttccg gacaagggga ctcattgcac gaacatattg cgaatcttgc tggttcgcca
     2281 gccatcaaaa agggcatact ccagacagtc aaagtagtgg atgagctagt taaggtcatg
     2341 ggacgtcaca aaccggaaaa cattgtaatc gagatggcac gcgaaaatca aacgactcag
     2401 aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat taaagaactg
     2461 ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa cgagaaactt
     2521 tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact ggacataaac
     2581 cgtttatctg attacgacgt cgatgcgatc gtaccccaat cctttttgaa ggacgattca
     2641 atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga caatgttcca
     2701 agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa tgcgaaactg
     2761 ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt gtctgaactt
     2821 gacaaggccg gatttattaa acgtcagctc gtggaaaccc gcgccatcac aaagcatgtt
     2881 gcgcagatac tagattcccg aatgaatacg aaatacgacg agaacgataa gctgattcgg
     2941 gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa
     3001 ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta tcttaatgcc
     3061 gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt tgtgtatggt
     3121 gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag
     3181 gctacagcca aatacttctt ttattctaac attatgaatt tctttaagac ggaaatcact
     3241 ctggcaaacg gagagatacg caaacgacct ttaattgaaa ccaatgggga gacaggtgaa
     3301 atcgtatggg ataagggccg ggacttcgcg acggtgagaa aagttttgtc catgccccaa
     3361 gtcaacatag taaagaaaac tgaggtgcag accggagggt tttcaaagga atcgattctt
     3421 ccaaaaagga atagtgataa gctcatcgct cgtaaaaagg actgggatcc aaaaaaatat
     3481 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
     3541 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga
     3601 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
     3661 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
     3721 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa
     3781 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat
     3841 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag
     3901 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt
     3961 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat
     4021 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt
     4081 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc
     4141 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga cggttctccc
     4201 aagaagaaga ggaaagtctc gagcgactac aaagaccatg acggtgatta taaagatcat
     4261 gacatcgatt acaaggatga cgatgacaag gctgcaggag gcggtggaag cgggcgcgcc
     4321 gacgcgctgg acgatttcga tctcgacatg ctgggttctg atgccctcga tgactttgac
     4381 ctggatatgt tgggaagcga cgcattggat gactttgatc tggacatgct cggctccgat
     4441 gctctggacg atttcgatct cgatatgtta catcaccacc accatcacta atgactcgag
     4501 tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc gtctactagc
     4561 gcagcttaat taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
     4621 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     4681 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4741 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4801 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4861 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4921 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4981 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5041 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5101 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttctggcggc acgatggcat
     5161 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5221 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5281 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5341 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5401 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5461 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5521 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5581 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5641 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5701 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5761 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5821 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5881 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5941 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6001 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6061 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6121 cttccttttt caatcatgat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6181 tatttgaatg tatttagaaa aataaacaaa taggtcatga ccaaaatccc ttaacgtgag
     6241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6421 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6901 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6961 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7021 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     7081 ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct
     7141 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     7201 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7261 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7321 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     7381 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     7441 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     7501 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     7561 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     7621 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     7681 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     7741 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg
     7801 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct
     7861 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc
     7921 tagccgggtc ctcaacgaca ggagcacgat catgctagtc atgccccgcg cccaccggaa
     7981 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
     8041 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8101 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8161 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     8221 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     8281 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     8341 ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     8401 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
     8461 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     8521 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     8581 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     8641 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     8701 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     8761 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     8821 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     8881 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     8941 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     9001 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     9061 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     9121 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     9181 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
     9241 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     9301 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     9361 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     9421 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     9481 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa
     9541 t
//
