LOCUS       sequence-194900-        9214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid. Note that both renilla luciferase and 
            ORF-firefly luciferase are encoded by a single mRNA and both are 
            translated independently via internal ribosomal binding sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: pCMV-IRES-Renilla Luciferase-IRES-Gateway-Fire...
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(16..590,591..593,594..602)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             603..1538
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    join(1626..2200,2201..2203,2204..2212)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     protein_bind    2232..2356
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2381..2386,2387..2404,2405..2411)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2465..3124
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2531..2550)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3466..3771
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3650..3669
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3812..3936)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3983..5635
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4049..4067)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5638..5862
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5995..6014)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6202..6323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6213..6232)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6267..6286
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6456..6560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6561..6629,6630..7421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6779..6798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7592..8180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8081..8100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(8280..8299)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8471..8850
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8851..9054
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9005..9025
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9051..9075
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ccggtatgta atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
       61 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
      121 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
      181 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
      241 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
      301 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
      361 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
      421 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
      481 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
      541 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
      601 ccatgacttc gaaagtttat gatccagaac aaaggaaacg gatgataact ggtccgcagt
      661 ggtgggccag atgtaaacaa atgaatgttc ttgattcatt tattaattat tatgattcag
      721 aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct tcttatttat
      781 ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca gaccttattg
      841 gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat cattacaaat
      901 atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt gtcggccatg
      961 attggggtgc ttgtttggca tttcattata gctatgagca tcaagataag atcaaagcaa
     1021 tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg cctgatattg
     1081 aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg gagaataact
     1141 tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca gaagaatttg
     1201 cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca ttatcatggc
     1261 ctcgtgaaat cccgttagta aaaggtggta aacctgacgt tgtacaaatt gttaggaatt
     1321 ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa tcggacccag
     1381 gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact gaatttgtca
     1441 aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga aaatatatca
     1501 aatcgttcgt tgagcgagtt ctcaaaaatg aacaatgact cgaggtttaa actatagagt
     1561 acgagccatg ataactgcag gtcgagcatg catctagggc ggccaattcc gcccctctcc
     1621 ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     1681 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     1741 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     1801 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     1861 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     1921 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     1981 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     2041 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     2101 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     2161 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga
     2221 gacgaccttc cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     2281 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     2341 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     2401 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     2461 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2521 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     2581 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     2641 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     2701 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     2761 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     2821 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     2881 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     2941 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     3001 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     3061 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     3121 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     3181 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     3241 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     3301 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     3361 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     3421 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     3481 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     3541 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     3601 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     3661 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     3721 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     3781 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     3841 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     3901 ttacgtttct cgttcagctt tcttgtacaa agtggtgggt ggaggcggtt caggtggcgg
     3961 aggaagcgga ggcggtggaa gtatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     4021 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     4081 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     4141 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     4201 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     4261 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     4321 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     4381 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     4441 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     4501 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     4561 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     4621 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     4681 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4741 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     4801 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     4861 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4921 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     4981 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     5041 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     5101 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     5161 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     5221 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     5281 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     5341 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     5401 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     5461 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     5521 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     5581 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaagactg
     5641 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5701 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5761 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5821 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5881 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5941 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6001 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6061 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6121 attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat tctctagagc
     6181 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     6241 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6301 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     6361 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     6421 ataaggatcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6781 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6901 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6961 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7021 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7081 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7141 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7201 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7261 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7321 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7381 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7441 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7501 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7921 catgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8161 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8221 gctggccttt tgctcacatg gctcgacaga tctcccgatc ccctatggtg cactctcagt
     8281 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     8341 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     8401 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     8461 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     8521 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     8581 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     8641 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     8701 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     8761 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     8821 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     8881 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     8941 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     9001 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     9061 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     9121 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacggacct gcaggaattg
     9181 atccgcggcc ctagcgctac cggactcaga tcca
//
