LOCUS       sequence-194904-        4794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the fluorescent protein Tam1 for arabinose-inducible 
            expression in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, 
            Hughes TE, Drobizhev M
  TITLE     Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than 
            Enhanced Green Fluorescent Protein under Two-Photon Excitation.
  JOURNAL   J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 
            10.1021/acs.jpclett.7b00960. Epub 2017 May 25.
  PUBMED    28530831
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jpclett.7b00960"; journalName: "J Phys Chem Lett"; 
            date: "2017-06-15- 15"; volume: "8"; issue: "12"; pages: "2548-2554"
COMMENT     Sequence Label: pBAD-Tam1
FEATURES             Location/Qualifiers
     source          1..4794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..294
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     217..236
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             340..357
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             361..393
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(397..405,406..420)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(1204..1221)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1374..1460
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1552..1579
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1598..1689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1690..1758,1759..2550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1908..1927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2721..3309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3210..3229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3463..3480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3495..3635
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3561..3580)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3721..3743
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3899..4777)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
       61 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
      121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
      181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
      241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
      301 tttttgggct aacaggagga attaaccatg gggggttctc atcatcatca tcatcatggt
      361 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
      421 gatccgagct cgagcatggt gagcaagggc gaggagacca caatgggcgt aatcaagccc
      481 gacatgaaga tcaagctgaa gatggagggc aacgtgaatg gccacgcctt cgtgatcgag
      541 ggcgagggcg agggcaagcc ctacgacggc accaacaccg tcaacctgga ggtgaaggag
      601 ggagcccccc tgcccttctc ctacgacatc ctgtccaacg ccttcctgta cggcaacagg
      661 gccttcacca agtaccccga cgacatcgcc aactacttca agcagtcctt ccccgagggc
      721 tactcctggg agcgcaccat gaccttcgag gacaagggca tcgtgaaggt gaagtccgac
      781 atctccatgg aggaggactc cttcatctac gagatccgcc tcaagggcaa gaacttcccc
      841 cccaacggcc ccgtgatgca gaagaagacc ctgaagtggg agccctccac cgagatcctg
      901 tacgtgcgcg acggcgtgct ggtgggcgac atctcccact ccctgctgct ggagggcggc
      961 ggccactacc gctgcgactt caagaccatc tacaaggcca agaaggtggt gaagctgccc
     1021 gactaccact tcgtggacca ccgcatcgag atcctgaacc acgacaagga ctacaacaag
     1081 gtgaccctgt acgagaacgc cgtggcccgc tactccctgc tgccccccca ggcaggcatg
     1141 gacgagctgt acaagtaaga attcgaagct tggctgtttt ggcggatgag agaagatttt
     1201 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
     1261 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     1321 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
     1381 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1441 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
     1501 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
     1561 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat
     1621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1921 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2221 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2401 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2581 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2641 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3121 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3361 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3481 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3541 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3601 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3661 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3721 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga
     3781 tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag
     3841 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt
     3901 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
     3961 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
     4021 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
     4081 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
     4141 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
     4201 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
     4261 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
     4321 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
     4381 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
     4441 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     4501 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     4561 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     4621 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     4681 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     4741 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgc
//
