LOCUS       sequence-194905-       14804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes
            the pylRS orthogonal translation system. In addition it encodes for 
            a GFP reporter with TAG mutation at site 35..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14804)
  AUTHORS   Chemla Y, Friedman M, Heltberg M, Bakhrat A, Nagar E, Schwarz R, 
            Jensen MH, Alfonta L
  TITLE     Expanding the Genetic Code of a Photoautotrophic Organism.
  JOURNAL   Biochemistry. 2017 Apr 12. doi: 10.1021/acs.biochem.7b00131.
  PUBMED    28394580
REFERENCE   2  (bases 1 to 14804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2017 Apr 12. doi: 10.1021/acs.biochem.7b00131."
COMMENT     Sequence Label: pCOTS-pyl-GFP(35TAG)
FEATURES             Location/Qualifiers
     source          1..14804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    113..134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(149..154,155..172,173..179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    187..203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     192..214
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     211..227
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     211..227
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        248..266
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     295..311
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1838..2053
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2054..2854
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPAAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             3117..3134
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3171..3192)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3432..3451)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3779..3800
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6297..6316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6298..6316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6323..6340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6323..6339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6332..6354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6480..6935)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6617..6638)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6829..6848
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6962..7066
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7396..7415)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7407..8222
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7407..8222
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7480..7499)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8639..9227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9128..9147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9867..10658
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     14783..14800
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agctcgaaat taaccctcac taaagggaac aaaagctggt accgggcccc ccctcgaggt
      301 cgacggtatc gataagcttg aacagcctgg gttcctcatg actctcagtc tcagctgcat
      361 gcgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg
      421 ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg
      481 acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa
      541 gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt
      601 atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac
      661 gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg
      721 atgcctttat cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag
      781 tggagtcagg ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct
      841 taacggacct acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga
      901 aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag
      961 aacaaaaaag aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt
     1021 tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt
     1081 tgttttacaa aaatgaagca cagattcttc gttggtaaga tagcgctttc gcgttgcatt
     1141 tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa agttagcgct ctcgcgttgc
     1201 atttttgttc tacaaaatga agcacagatg cttcgttaac aaagatatgc tattgaagtg
     1261 caagatggaa acgcagaaaa tgaaccgggg atgcgacgtg caagattacc tatgcaatag
     1321 atgcaatagt ttctccagga accgaaatac atacattgtc ttccgtaaag cgctagacta
     1381 tatattatta tacaggttca aatatactat ctgtttcagg gaaaactccc aggttcggat
     1441 gttcaaaatt caatgatggg taacaagtac gatcgtaaat ctgtaaaaca gtttgtcgga
     1501 tattaggctg tatctcctca aagcgtattc gaatatcatt gagaagctgc agcgtcacat
     1561 cggataataa tgttggcagc cattgtagaa gtgccttttg catttctagt ctctttctcg
     1621 gtctagctag ttttactaca tcgcgaagat agaatcttag atcacactgc ctttgctgag
     1681 ctggatcaat agagtaacaa aagagtggta aggcttcgtt aaaggacaag gacctgagcg
     1741 gaagtgtatc gtacagtaga cggagtatac taggtatagt ctatagtccg tggaattaat
     1801 tctcatgttt gacagcttat catcgataag ctagcttttc aattcatcat ttttttttta
     1861 ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct ccgaacagaa
     1921 ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga
     1981 agaaacatga aattgcccag tattcttaac ccagctgcac agaacaaaaa cctgcaggaa
     2041 acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc
     2101 tgctgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt
     2161 ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg
     2221 tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc
     2281 gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga
     2341 cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc
     2401 agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc
     2461 ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa
     2521 gggctcccta tctactggag agtatactaa gggtactgtt gacattgcga agagcgacaa
     2581 agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga
     2641 ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat tgggtcaaca
     2701 gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg ttggaagagg
     2761 actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg
     2821 ggaagcatat ttgagaagat gcggccagca aaactgcatg cgctggattt agcgtcttct
     2881 aatccagtgt agacagtagt tttggctccg ttgagcactg tagccttggg cgatcgctct
     2941 aaacattaca taaattcaca aagttttcgt tacataaaaa tagtgtctac ttagctaaaa
     3001 attaagggtt ttttacacct ttttgacagt taatctccta gcctaaaaag caagagtttt
     3061 taactaagac tcttgccctt tacaacctca agatcgatac tagtatgggc agcagccatc
     3121 atcatcatca tcacagctct agagagcttt tcactggcgt tgttcccatc ctggtcgagc
     3181 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3241 cctagggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3301 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3361 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3421 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3481 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3541 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3601 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3661 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3721 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3781 tggtcctgct ggagttcgtg accgccgccg ggatctaatc tagagcggcc gcgaattccc
     3841 gtttgactgg cgatgctgct actgaaacta acaactacat cgactacgca attaacgccc
     3901 tcagctaatt ttgcttagtc taggcccgga tgggtaagtg gttttcagct taagtgttgg
     3961 gttctactta cttctccggg tcttgctcta tctaaaaaca ttggtttaac aaggagtatt
     4021 aggcaaatgc cagttactgt cgctgcctct cgcttgggaa ccgctgcgtt tgaccaatca
     4081 cccgtcgaac tgcgcgctaa ctattctcga cctgcaggtc gacggtatcg atcccgggcc
     4141 tgtctaggca gtagtagaca actcagttgc tgacctcaga gactgcttga caaaattttc
     4201 tatcggacta aagtaattaa ttgtgcggtt cgcacttgcc aggaaacctg atcatgtaga
     4261 tcgaatggac tctaaatccg ttcagccggg ttagattccc ggggtttccg ccattctttc
     4321 ttacccctgt catcaatggt gataggggtt cgttttttgt tatctgtgac tattgcccac
     4381 gcagtaggaa tctggctgtc catggcagtg ggcggaatct catccgccgg tatcgataag
     4441 cttgaaacaa aacgtttaaa actttatgta ataacaaatt taaatatgta agttaagaac
     4501 tttcaaagaa taacttatgc catttcttga tatattgtga gacaagttac aaattacgtg
     4561 gtgtgcaatt ttttcatctt gcgctgatta ctctactaaa tatccgtcaa gtaaattggc
     4621 tcttagctcg tctcctgtca ataaaggagg tcggcaagag tgcagaagcg ggaatgtgtg
     4681 aaaactaacc caattcatta aataccccga aatatagggg aatcatctca tactttccgt
     4741 aaaccgcgaa ggtcgtgaag ggataaaagc aatttagtgg gtgagaagaa cagataaaaa
     4801 agaatttttt aactatggca agaggaaaaa gtaaaagcgt tatcgaattc ctgcagcccg
     4861 ggggatcctc tagatttaag aaggagatat acatatggat aaaaaaccac taaacactct
     4921 gatctctgct actggtctgt ggatgagtcg taccggaacc attcataaaa tcaaacacca
     4981 cgaggttagc cgttcgaaaa tctatattga gatggcgtgt ggcgatcatc tggttgtgaa
     5041 caatagccgc tcttctcgta cagcacgtgc actgcgtcac cacaaatatc gtaaaacctg
     5101 taaacgttgc cgtgtgtccg atgaggatct gaacaaattc ctgacaaaag ccaatgagga
     5161 ccaaacaagc gtgaaagtga aagtcgttag cgctcctacc cgtactaaaa aagcaatgcc
     5221 gaaatccgtt gctcgtgccc ctaaaccact ggaaaacact gaagcagcac aggcacagcc
     5281 gtctggaagc aaattctctc cggccattcc tgtttctacc caggagtccg tttctgttcc
     5341 agcaagtgtg agcaccagca ttagcagtat tagcaccggt gccaccgcta gcgccctggt
     5401 taaaggcaat accaatccga ttacaagcat gtctgccccg gttcaagcat cagctccagc
     5461 actgacaaaa tcccaaaccg atcgtctgga ggttctgctg aatccgaaag acgaaatcag
     5521 cctgaattcc ggcaaaccgt ttcgtgaact ggagagcgaa ctgctgtcac gtcgtaaaaa
     5581 agacctgcaa caaatctatg ccgaagaacg tgagaactat ctggggaaac tggaacgtga
     5641 aatcacccgc tttttcgtgg atcgtggctt tctggagatc aaatccccga ttctgattcc
     5701 tctggagtat atcgagcgta tgggcatcga caatgatacc gaactgagca aacaaatttt
     5761 ccgtgtggat aaaaacttct gtctgcgccc tatgctggca ccaaatctgt ataactatct
     5821 gcgcaaactg gaccgtgccc tgcctgatcc tatcaaaatc ttcgagatcg gcccgtgtta
     5881 tcgtaaagag tccgacggta aagaacatct ggaggagttt accatgctga acttttgcca
     5941 aatgggttca ggttgtactc gtgagaacct ggaaagcatc atcaccgatt ttctgaacca
     6001 cctgggcatt gacttcaaaa ttgtgggcga cagctgtatg gtgtatggcg acaccctgga
     6061 tgtcatgcac ggcgacctgg aactgtctag tgccgttgtt ggaccaattc cgctggaccg
     6121 tgagtggggt atcgacaaac cgtggatcgg agcaggattc ggtctggaac gcctgctgaa
     6181 agtgaaacac gacttcaaaa acatcaaacg tgccgcccgt tctgaatcgt attataacgg
     6241 gatttctacc aacctgtaag tcgacgcggc cgccaccgcg gtggagctcc aattcgccct
     6301 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     6481 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     6541 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     6601 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     6661 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     6721 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     6781 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     6841 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     6901 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7021 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7081 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7141 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7201 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7261 aatgatgagc acttttgacc tgcagggggg ggggggaaag ccacgttgtg tctcaaaatc
     7321 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     7381 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
     7441 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     7501 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     7561 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     7621 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     7681 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
     7741 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     7801 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     7861 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     7921 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     7981 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     8041 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     8101 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     8161 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     8221 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     8281 gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc
     8341 cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta
     8401 caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca
     8461 ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgcc cccccccccc
     8521 tgcaggtcaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccat
     8701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8761 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgccc gttccataca
     9301 gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat
     9361 ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc
     9421 agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac
     9481 gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt
     9541 cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     9601 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     9661 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     9721 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     9781 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     9841 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     9901 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     9961 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
    10021 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
    10081 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
    10141 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
    10201 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
    10261 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
    10321 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
    10381 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
    10441 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
    10501 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
    10561 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
    10621 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
    10681 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
    10741 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
    10801 acacaaattg ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc
    10861 gaagtaatcg caacatccgc attaaaatct agcgagggct ttactaagct gatccggtgg
    10921 atgacctttt gaatgacctt taatagatta tattactaat taattgggga ccctagaggt
    10981 cccctttttt attttaaaaa ttttttcaca aaacggttta caagcataaa gcttgctcaa
    11041 tcaatcaccg gatctgcaag tcaacagccg cgatgagttc aacccttggt cgctggaggt
    11101 tgcgatcgac gcacggctga gcggcagcta ctactaccta gttgcacgcg gcaacctacc
    11161 tctggagctg ggacgcctga cgcccgcgcc tcagctaact agcgaggtgc agttcgagac
    11221 gggcttttac cgcgccaagg ctgagcacag cttcggctgc tgcgtagtcg atcaccgtcc
    11281 gatcgttcgg atctcgacag cgtccggcgt ctaagtctca tcacagccag gggcgatcgc
    11341 gcctctggct tgccgatcaa ttcgaggcag ccttgccacc tgccgtattc attccagcta
    11401 tagttatcgg caatcaaagc aaaacccccc ccaggccagg tcggcaaact ttgcccaggg
    11461 gaggtcagga aaaataactc aacagaagta tggcatttat cgccaatcat ctgcaaaggt
    11521 cgcagatccc gacccgctgc gaattaattg atcaacttga ccggctgccc ccaaactggc
    11581 gcttctgcgt cgttaacagt cgcaaggcac catttgaccc tggctggcaa aataacccgc
    11641 ttgaccatgc tgctgttgct gaacgtatcc gcagcgatcg gcgcgttacc ggaatcggtc
    11701 tgcttactgg ccccgcaagc ggtgggttaa tcgcagttga cttcgatggc ccaacggctc
    11761 atgaggcact gccagaaggg ctgacgctga cagaattacc gcccacagtt gcatatacat
    11821 ctggcaagcc aggacgccac caacggctct atcaggtacc ccaagagcga tgggctgcga
    11881 tcgccacgca gaagcttcac agcccagacg gtgatctgtt agagctgcgt tggaacaagc
    11941 tgcaatcagt catcgttggt caacaccctg agaccggcgc atatcgctgg gtggaaggct
    12001 gcgcaccttg ggagatcgag gttgcagagg ccccgcccga attgctggat gcaatggagc
    12061 ggcaagaccg gcagaccgcg ccgaaatcct ataagccgat cgtcagtgct ccagcttctg
    12121 atgatgaggt cgcgatcgcc cgcaccatgc tggcccatgt acctgccagc tacgcagacg
    12181 actacgagag ctgggtggct gtcgggatgg ccctgcagtc agtcagcgat gccctgttag
    12241 atgactggat tgcatggtca gcgcaatcgt ccaagttcga cggcaaccgc aagctagagc
    12301 ggaagtgggc cagcttcaag ggcagcggca tcacgatcgc cacactggcc aagctggcca
    12361 aagaaggcgg ctaccgcccg ccaaagctga ctcgacaaga gcagcgggaa tatcagcgcg
    12421 aggtcgcgaa gcagtggcag cggctgaccc gtagccctaa ccgcttacac accggcgggt
    12481 acatcaacgg cgactacatc ccgcagccgg aacagcatcg ccttgtctgc ctgaatgctg
    12541 cgatggggac gggcaagaca gaggcgatcg cggggcatct ccagccgctc attggtaccg
    12601 gcattcctgt cgtgttgatc acgcatcgcc gtaccttggg cgcggccttg ggcaagcgct
    12661 taggggtgcc gtgggctgaa gaatgcgttc ccggttccga ccttcggatg atcggccttg
    12721 gcggttgcct agactccatg caccccgcca gcaagctgcg cttcagcgcc cacggatggc
    12781 aacgggcgat cgtcgtcatc gatgaggtgg aactgtgggc tgagcacctg ctgagcggca
    12841 agaccgagat cgctaaacat cgccccgaag tcatggctga aattgctgga ctactcgcag
    12901 gggggcggca ggttatcgcc gctgacgcga tgctgtcgga tgtgtcgatc gccctgttgg
    12961 aagcactgac aggatcaacc gcatatctgg tcagcagtac cgtccaaccc ttcagcggtg
    13021 ctccggtcta tcaggttggc agcgatcgcg aactcatggc gatggcggtc gaaatggttc
    13081 aggcgggcaa gcgcttagca gtcttctgcg accgccagaa gctgagccag tcgcggccgt
    13141 ccctgtattc cgcagcgacg atcgcccaac agctctccga agcctgcggt cagccggttc
    13201 cggtcatcga cagcgaagcc aataagcggg gggctttgga aacgctgaag ctcaacccgc
    13261 agatgttcat caactgttgc ccccaccttg tcttcacgag cgtgatcgat gctggcttgt
    13321 cgatcaccca aggggtaggc gatcgcccat ttgatgccgt gctgatcttc gccggagctg
    13381 ggcacatcgc cccaggtgcg atcgcgcaga ttgcaggacg ggtgcggagc aacgtccccc
    13441 gattcatcac ctgccccgac acgtccaatt tgatgcgagc tggcaacggt agcatcgacc
    13501 caaccgaggt tgccgacggc ctgaagcggc accaacaagt gatcttggct cagctgtcac
    13561 aggcggcgat caagatcggc gagccggtcg catcgcaagc ctacttgact gcatgggcag
    13621 aattggccgc ccgtcgcaac cgtgacgggt tgacctatgc cgccacggtg gagcagcttc
    13681 tacggctcaa cgggttcact tggcaagact gggacggcgc gatcgatccc tttcttcaag
    13741 aggtcgttga cgaactgcaa cgggagactg ctgaagcgct ggctgatgcc tcagccgagg
    13801 cgatcctgag ttcacctgat ctgaccccga aggaagctga ggcgatcgcg tctgcggact
    13861 tcgctaacgc ggaacagttg gctgcccttg ctcggcaccg ctgccagcaa cgctggggag
    13921 actgcacccg tgaactacta gccgctgatg acgacggtct atacgccccg ctaagactgc
    13981 tgttctggct cagccttggc aaggatgccg ctgccgctcg cgatgccgcc aagctcgaca
    14041 aacggcgcga tcgcccgatg ttcggacccg atcttgtggg cagctgtatc gcgccccaag
    14101 tgcggtacct gcatgggctg aaggtggaag agttgctgca acgccgcgac tggttcagcg
    14161 ttgatgatcc gctgattcaa gagatcgtca cgaccgccac aagccaccgg gctagtgccg
    14221 cacagatcct caagattcag atcaccgaca agaccagagc cagcaccatc gtgcgggcgc
    14281 tgctgcggat gatcggcttc aagctcaagg cacggcagaa gcgcatcgcg ggcaagtcca
    14341 cttggatcta ccaagtcgtg ccgaatgagg cgattgctga actgccgctt gaggcgatcg
    14401 aagctcattg gcttgaaaac gcccgatcta gtcagggact ggggagctgt cacgaaatcc
    14461 ctaatagaag atcaaaacca atcactgcgc attttcgtga caccgcgtca ggtcaggtca
    14521 gcactacatc tacggagacc gcagcatgac acaagccgaa ctcgatcagc tttggacaac
    14581 tgcgatcgcc gcgatcgaga acgaaggctt gcgatggttt gcggctaacc gctgccgcgc
    14641 ctacagcttc gatagtggaa cgctggccat cgcagcaccc aagcacatgt tctttcctgc
    14701 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    14761 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaag
//
