LOCUS       sequence-19494-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pc3XB-ZF11
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(222..239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    228..452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(585..604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
       61 gtttaaacgg gccctctaga cccggggaga agccgcatat ttgccacatc caaggctgtg
      121 ggaaggtgta cggccgtagc gatcacctgg cacgtcactt gcgctggcat accggtggat
      181 cctctgcaca gtagtaagct taagtttaaa ccgctgatca gcctcgactg tgccttctag
      241 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      301 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      361 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      421 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
      481 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      541 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      601 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctct
//
