LOCUS       sequence-194940-        2538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mini-transposon system suitable for sequencing, shuttle mutagenesis 
            and gene fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Kahrs AF, Odenbreit S, Schmitt W, Heuermann D, Meyer TF, Haas R
  TITLE     An improved TnMax mini-transposon system suitable for sequencing, 
            shuttle mutagenesis and gene fusions.
  JOURNAL   Gene. 1995 Dec 29;167(1-2):53-7.
  PUBMED    8566811
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1995-12-29"; volume: "167(1-2)"; pages: "53-7"
COMMENT     Sequence Label: pMin1
FEATURES             Location/Qualifiers
     source          1..2538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             186..1376
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(538..557)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            1448..1557
                     /note="incP origin of transfer"
     rep_origin      1783..2371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2272..2291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aattcagatc tcgaggtacc cgggattctc tagatcgatg tcgacaagat tgatgtcgat
       61 aagtttagcc cgcctaatga gcgggctttt tttttgatct aattcatgtt tgacattgcc
      121 atatagatga gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac
      181 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg
      241 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg
      301 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat
      361 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt
      421 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc
      481 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      541 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      601 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      661 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      721 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
      781 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
      841 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
      901 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
      961 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     1021 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     1081 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     1141 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     1201 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt
     1261 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     1321 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     1381 aagccggcgg cctgcagctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
     1441 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     1501 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     1561 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     1621 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     1681 gcagcggaaa agcgctgctt ccctgctgtt ttgtggaata tctaccgact ggaaacaggc
     1741 aaatgcagga aattactgat ctgcagagat caaaggatct tcttgagatc ctttttttct
     1801 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1861 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1921 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1981 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2041 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2101 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2161 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2221 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2281 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2341 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2401 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2461 ggataaccgt attaccgcct ttgagtgagc tgatacgaat tagatcatta aaggctcctt
     2521 ttggagcctt tttttttg
//
